ID   MCA7_ARATH              Reviewed;         403 AA.
AC   Q6XPT5; O64520; Q56X59; Q7XJE4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Metacaspase-7;
DE            Short=AtMC7;
DE            EC=3.4.22.-;
DE   AltName: Full=Metacaspase 2a;
DE            Short=AtMCP2a;
DE   AltName: Full=Metacaspase-4;
GN   Name=AMC7; Synonyms=AMC4, MCP2A; OrderedLocusNames=At1g79310;
GN   ORFNames=YUP8H12R.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AUTOCATALYTIC CLEAVAGE, GENE FAMILY, AND
RP   NOMENCLATURE.
RX   PubMed=15326173; DOI=10.1074/jbc.m406329200;
RA   Vercammen D., van de Cotte B., De Jaeger G., Eeckhout D., Casteels P.,
RA   Vandepoele K., Vandenberghe I., van Beeumen J., Inze D., van Breusegem F.;
RT   "Type II metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana cleave
RT   substrates after arginine and lysine.";
RL   J. Biol. Chem. 279:45329-45336(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ikeda Y., Krishnamurthy N., Chua N.-H.;
RT   "Characterization of metacaspases.";
RL   Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY.
RX   PubMed=20565583; DOI=10.1111/j.1364-3703.2004.00206.x;
RA   Watanabe N., Lam E.;
RT   "Recent advance in the study of caspase-like proteases and Bax inhibitor-1
RT   in plants: their possible roles as regulator of programmed cell death.";
RL   Mol. Plant Pathol. 5:65-70(2004).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=21395887; DOI=10.1111/j.1365-313x.2011.04554.x;
RA   Watanabe N., Lam E.;
RT   "Arabidopsis metacaspase 2d is a positive mediator of cell death induced
RT   during biotic and abiotic stresses.";
RL   Plant J. 66:969-982(2011).
CC   -!- TISSUE SPECIFICITY: Expressed in roots, flowers and siliques.
CC       {ECO:0000269|PubMed:21395887}.
CC   -!- PTM: Proteolytically processed; by an autocatalytic mechanism.
CC   -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC17037.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAD94017.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR   EMBL; AY219832; AAP44520.1; -; mRNA.
DR   EMBL; AY322528; AAP84709.1; -; mRNA.
DR   EMBL; AC002986; AAC17037.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE36229.1; -; Genomic_DNA.
DR   EMBL; AK221818; BAD94017.1; ALT_SEQ; mRNA.
DR   PIR; T01024; T01024.
DR   RefSeq; NP_178049.2; NM_106579.5.
DR   AlphaFoldDB; Q6XPT5; -.
DR   SMR; Q6XPT5; -.
DR   STRING; 3702.AT1G79310.1; -.
DR   MEROPS; C14.A03; -.
DR   PaxDb; Q6XPT5; -.
DR   PRIDE; Q6XPT5; -.
DR   ProteomicsDB; 238889; -.
DR   EnsemblPlants; AT1G79310.1; AT1G79310.1; AT1G79310.
DR   GeneID; 844269; -.
DR   Gramene; AT1G79310.1; AT1G79310.1; AT1G79310.
DR   KEGG; ath:AT1G79310; -.
DR   Araport; AT1G79310; -.
DR   TAIR; locus:2207450; AT1G79310.
DR   eggNOG; KOG1546; Eukaryota.
DR   HOGENOM; CLU_029389_4_1_1; -.
DR   InParanoid; Q6XPT5; -.
DR   OMA; CNDRFVN; -.
DR   OrthoDB; 792090at2759; -.
DR   PhylomeDB; Q6XPT5; -.
DR   PRO; PR:Q6XPT5; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q6XPT5; baseline and differential.
DR   Genevisible; Q6XPT5; AT.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR029030; Caspase-like_dom_sf.
DR   SUPFAM; SSF52129; SSF52129; 1.
PE   1: Evidence at protein level;
KW   Autocatalytic cleavage; Hydrolase; Protease; Reference proteome;
KW   S-nitrosylation; Thiol protease.
FT   CHAIN           1..403
FT                   /note="Metacaspase-7"
FT                   /id="PRO_0000334605"
FT   ACT_SITE        86
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000250"
FT   MOD_RES         139
FT                   /note="S-nitrosocysteine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FYE1"
FT   CONFLICT        270
FT                   /note="D -> G (in Ref. 5; BAD94017)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        303
FT                   /note="Q -> H (in Ref. 2; AAP84709)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   403 AA;  45186 MW;  DF592AFBB42F49D2 CRC64;
     MAKRALLIGI NYPGTTEELQ GCVNDVHRMH KCLVDRFGFA EEDITVLIDT DESYTQPTGK
     NIRQALSELI KPAKSGDVLF VHYSGHGTRV PPETGEEDDT GFDECIVPSD LNPIPDDDFR
     DLVEQVPEGC QITIVSDSCH SGGLIDEAKE QIGESTTTKP NRESKVSSFE FEFKNCLHSI
     FVKLLAFCGI GSSHVETREI VEVGEGDEVV RSRYLPLERF IELLKQQTGQ DNIEIGKIRP
     TLFDVFGEDS SPKIKKFMKV ILTKLRKTND QSTLLGKIEE SARGYIEETL NDEHYMKPAM
     QAQVKSDREI YGGRSSNGLF PDRGILLSGC QTDETSADVK KKGEAFGAFS NAIQMVLSET
     DHKDKITNKE MVLRAREILK KQMFIQRPGL YCNDRFVNAP FIC