A synthetic peptide inhibitor of PKA
货号:ajcx23834
CAS:N/A
分子式:C94H148N32O31•XCF3COOH
分子量：2222.4
溶解度:DMSO: 1 mg/ml,PBS (pH 7.2): 2 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

PKI PKA Inhibitor (5-24) is a synthetic peptide inhibitor of PKA (cAMP-dependent protein kinase) (K_i= 2.3 nM) derived from the active site of the skeletal muscle inhibitor protein.¹It mimics the protein substrate by binding to the catalytic site through the arginine-cluster basic subsite.¹The prominent enzyme-substrate interaction site occurs where PKA catalytic subunit residues Tyr²³⁵and Phe²³⁹form a sandwich-like structure with residue Phe¹⁰of PKI (5-24).²

1.Cheng, H.C., Kemp, B.E., Pearson, R.B., et al.A potent synthetic peptide inhibitor of the cAMP-dependent protein kinaseThe Journal of Biological Chemisty261(3)989-992(1986) 2.Bossemeyer, D., Engh, R.A., Kinzel, V., et al.Phosphotransferase and substrate binding mechanism of the cAMP-dependent protein kinase catalytic subunit from porcine heart as deduced from the 2.0 Å structure of the complex with Mn2+ adenylyl imidodiphosphate and inhibitor peptide PKI(5-24)EMBO Journal12(3)849-859(1993)