ID   CYBT1_CATRO             Reviewed;         512 AA.
AC   W8JCE8;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Cytochrome P450 71BT1 {ECO:0000303|PubMed:24710322};
DE            Short=CrCYP71BT1 {ECO:0000303|PubMed:24710322};
DE            EC=1.14.-.- {ECO:0000305};
DE   Flags: Precursor;
GN   Name=CYP71BT1 {ECO:0000303|PubMed:24710322};
GN   Synonyms=CYP71B34 {ECO:0000305}; ORFNames=Caros004638 {ECO:0000305};
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Little Bright Eyes;
RX   PubMed=24710322; DOI=10.1038/ncomms4606;
RA   Miettinen K., Dong L., Navrot N., Schneider T., Burlat V., Pollier J.,
RA   Woittiez L., van der Krol S., Lugan R., Ilc T., Verpoorte R.,
RA   Oksman-Caldentey K.M., Martinoia E., Bouwmeester H., Goossens A.,
RA   Memelink J., Werck-Reichhart D.;
RT   "The seco-iridoid pathway from Catharanthus roseus.";
RL   Nat. Commun. 5:3606-3606(2014).
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=ORCAE database;
CC       URL="https://bioinformatics.psb.ugent.be/orcae/overview/Catro";
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DR   EMBL; KF302073; AHK60840.1; -; mRNA.
DR   AlphaFoldDB; W8JCE8; -.
DR   SMR; W8JCE8; -.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..512
FT                   /note="Cytochrome P450 71BT1"
FT                   /id="PRO_0000446414"
FT   BINDING         447
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        111
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        168
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   512 AA;  58923 MW;  237A71DB3BF07ADA CRC64;
     MENLFIFLFA LLLFCFMLLK LSKKYSALKL PPGPRPLPII GNLHQIGGGL MHHILREMAR
     TYGPLFRIKF GQVSTIVVSS PEIAKEIFRT HDVVFSYRPQ NIAVFNVMTY NFSNIAFAPY
     GNYWRQMRKI CVMEILGASR VRSFGSIRQE EVMNLIRFVG SHKGKLVNVS EKIYGLTYSI
     TARAAFGKVS KYQQVFKEHM DKYDDLARGL DIADFYPSMK FLRLVTGMER KLKVMFKEVD
     EILQVIIDEH RDQRMKKIRS KVDDNEKEEE EGNEDIVDVL MNLQQSGQTE LPVTDDNIKA
     VILDIFGAGG DTTAATLEWA LAESLKNKQV LKRAQEELRT IFHSKRNVDE SGFNQLKYLP
     AIVKETLRLH PPAPLSLPRE CSEECNIYGY DIPAKTRVMV NIWAMGRDPK YWSEPEEFKP
     ERFIDSRIDY KGNDFEYIPF GAGRRICPGM SFAIPNVALP LAQLLFHFDW KVDDDAGKLE
     DLDMVEHPVL EARRKNELKL IPVIYEGSCL KN