CYBH_ECOLI
ID CYBH_ECOLI Reviewed; 235 AA.
AC P0AAM1; P19929;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit;
GN Name=hyaC; OrderedLocusNames=b0974, JW0956;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2180913; DOI=10.1128/jb.172.4.1969-1977.1990;
RA Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S.,
RA Przybyla A.E.;
RT "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1
RT operon containing six open reading frames.";
RL J. Bacteriol. 172:1969-1977(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Probable b-type cytochrome.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the HupC/HyaC/HydC family. {ECO:0000305}.
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DR EMBL; M34825; AAA23999.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74059.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35739.1; -; Genomic_DNA.
DR PIR; JV0074; BVECYC.
DR RefSeq; NP_415493.1; NC_000913.3.
DR RefSeq; WP_001186424.1; NZ_STEB01000006.1.
DR PDB; 4GD3; X-ray; 3.30 A; A/B=1-235.
DR PDBsum; 4GD3; -.
DR AlphaFoldDB; P0AAM1; -.
DR SMR; P0AAM1; -.
DR BioGRID; 4260044; 42.
DR ComplexPortal; CPX-281; Hydrogenase-1 complex.
DR DIP; DIP-35859N; -.
DR IntAct; P0AAM1; 3.
DR STRING; 511145.b0974; -.
DR jPOST; P0AAM1; -.
DR PaxDb; P0AAM1; -.
DR PRIDE; P0AAM1; -.
DR EnsemblBacteria; AAC74059; AAC74059; b0974.
DR EnsemblBacteria; BAA35739; BAA35739; BAA35739.
DR GeneID; 66670750; -.
DR GeneID; 945581; -.
DR KEGG; ecj:JW0956; -.
DR KEGG; eco:b0974; -.
DR PATRIC; fig|1411691.4.peg.1300; -.
DR EchoBASE; EB0465; -.
DR eggNOG; COG1969; Bacteria.
DR HOGENOM; CLU_075520_0_0_6; -.
DR InParanoid; P0AAM1; -.
DR OMA; FYIAYPF; -.
DR PhylomeDB; P0AAM1; -.
DR BioCyc; EcoCyc:HYAC-MON; -.
DR BioCyc; MetaCyc:HYAC-MON; -.
DR PRO; PR:P0AAM1; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0044569; C:[Ni-Fe] hydrogenase complex; IDA:EcoCyc.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0098567; C:periplasmic side of plasma membrane; IDA:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IDA:EcoCyc.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0019645; P:anaerobic electron transport chain; IDA:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IDA:ComplexPortal.
DR GO; GO:0009267; P:cellular response to starvation; IDA:ComplexPortal.
DR GO; GO:0006113; P:fermentation; IDA:ComplexPortal.
DR InterPro; IPR011577; Cyt_b561_bac/Ni-Hgenase.
DR InterPro; IPR016174; Di-haem_cyt_TM.
DR InterPro; IPR000516; Ni-dep_Hydgase_cyt-B.
DR Pfam; PF01292; Ni_hydr_CYTB; 1.
DR PRINTS; PR00161; NIHGNASECYTB.
DR SUPFAM; SSF81342; SSF81342; 1.
DR TIGRFAMs; TIGR02125; CytB-hydogenase; 1.
DR PROSITE; PS00882; NI_HGENASE_CYTB_1; 1.
DR PROSITE; PS00883; NI_HGENASE_CYTB_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Electron transport; Heme;
KW Iron; Membrane; Metal-binding; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..235
FT /note="Probable Ni/Fe-hydrogenase 1 B-type cytochrome
FT subunit"
FT /id="PRO_0000201384"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..63
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..185
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT HELIX 20..40
FT /evidence="ECO:0007829|PDB:4GD3"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:4GD3"
FT HELIX 55..81
FT /evidence="ECO:0007829|PDB:4GD3"
FT TURN 82..85
FT /evidence="ECO:0007829|PDB:4GD3"
FT TURN 106..108
FT /evidence="ECO:0007829|PDB:4GD3"
FT STRAND 109..113
FT /evidence="ECO:0007829|PDB:4GD3"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:4GD3"
FT HELIX 133..151
FT /evidence="ECO:0007829|PDB:4GD3"
FT TURN 152..155
FT /evidence="ECO:0007829|PDB:4GD3"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:4GD3"
FT HELIX 164..173
FT /evidence="ECO:0007829|PDB:4GD3"
FT HELIX 178..207
FT /evidence="ECO:0007829|PDB:4GD3"
SQ SEQUENCE 235 AA; 27597 MW; 98048DC800AB02C7 CRC64;
MQQKSDNVVS HYVFEAPVRI WHWLTVLCMA VLMVTGYFIG KPLPSVSGEA TYLFYMGYIR
LIHFSAGMVF TVVLLMRIYW AFVGNRYSRE LFIVPVWRKS WWQGVWYEIR WYLFLAKRPS
ADIGHNPIAQ AAMFGYFLMS VFMIITGFAL YSEHSQYAIF APFRYVVEFF YWTGGNSMDI
HSWHRLGMWL IGAFVIGHVY MALREDIMSD DTVISTMVNG YRSHKFGKIS NKERS
