CYBC1_MOUSE
ID CYBC1_MOUSE Reviewed; 187 AA.
AC Q3TYS2; A2AN24; A2AN25; Q8VD13;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Cytochrome b-245 chaperone 1 {ECO:0000305};
DE AltName: Full=Essential for reactive oxygen species protein {ECO:0000303|PubMed:28351984};
DE Short=Eros {ECO:0000303|PubMed:28351984};
GN Name=Cybc1 {ECO:0000312|MGI:MGI:2384959};
GN Synonyms=Eros {ECO:0000303|PubMed:28351984};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=BALB/cJ, C57BL/6J, and NOD; TISSUE=Inner ear, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=28351984; DOI=10.1084/jem.20161382;
RA Thomas D.C., Clare S., Sowerby J.M., Pardo M., Juss J.K., Goulding D.A.,
RA van der Weyden L., Storisteanu D., Prakash A., Espeli M., Flint S.,
RA Lee J.C., Hoenderdos K., Kane L., Harcourt K., Mukhopadhyay S., Umrania Y.,
RA Antrobus R., Nathan J.A., Adams D.J., Bateman A., Choudhary J.S.,
RA Lyons P.A., Condliffe A.M., Chilvers E.R., Dougan G., Smith K.G.;
RT "Eros is a novel transmembrane protein that controls the phagocyte
RT respiratory burst and is essential for innate immunity.";
RL J. Exp. Med. 214:1111-1128(2017).
CC -!- FUNCTION: Functions as a chaperone necessary for a stable expression of
CC the CYBA and CYBB subunits of the cytochrome b-245 heterodimer (By
CC similarity). Controls the phagocyte respiratory burst and is essential
CC for innate immunity (PubMed:28351984). {ECO:0000250|UniProtKB:Q9BQA9,
CC ECO:0000269|PubMed:28351984}.
CC -!- SUBUNIT: Interacts with CYBB; CYBC1 may act as a chaperone stabilizing
CC Cytochrome b-245 heterodimer. {ECO:0000250|UniProtKB:Q9BQA9}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000305|PubMed:28351984}; Single-pass membrane protein
CC {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Mutants are highly susceptible to Salmonella and
CC Listeria infection and are impaired in their ability to control
CC replication of either pathogen (PubMed:28351984). Mutant neutrophiles
CC have a highly impaired superoxide burst (PubMed:28351984). Mice are
CC very susceptible to Listeria monocytogenes and die within 5 days of
CC infection, fail to form neutrophil extracellular traps but are
CC resistant to melanoma metastasis (PubMed:28351984).
CC {ECO:0000269|PubMed:28351984}.
CC -!- SIMILARITY: Belongs to the CYBC1 family.
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DR EMBL; AK145884; BAE26724.1; -; mRNA.
DR EMBL; AK158402; BAE34490.1; -; mRNA.
DR EMBL; AK159453; BAE35096.1; -; mRNA.
DR EMBL; AK168104; BAE40075.1; -; mRNA.
DR EMBL; AK170767; BAE42017.1; -; mRNA.
DR EMBL; AL808021; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC017643; AAH17643.1; -; mRNA.
DR EMBL; BC026616; AAH26616.1; -; mRNA.
DR EMBL; BC027406; AAH27406.1; -; mRNA.
DR CCDS; CCDS25771.1; -.
DR RefSeq; NP_001239477.1; NM_001252548.1.
DR RefSeq; NP_001239478.1; NM_001252549.1.
DR RefSeq; NP_001239479.1; NM_001252550.1.
DR RefSeq; NP_001241664.1; NM_001254735.1.
DR RefSeq; NP_659081.1; NM_144832.2.
DR AlphaFoldDB; Q3TYS2; -.
DR BioGRID; 229904; 2.
DR IntAct; Q3TYS2; 1.
DR MINT; Q3TYS2; -.
DR STRING; 10090.ENSMUSP00000042277; -.
DR iPTMnet; Q3TYS2; -.
DR PhosphoSitePlus; Q3TYS2; -.
DR EPD; Q3TYS2; -.
DR MaxQB; Q3TYS2; -.
DR PaxDb; Q3TYS2; -.
DR PeptideAtlas; Q3TYS2; -.
DR PRIDE; Q3TYS2; -.
DR Antibodypedia; 53358; 136 antibodies from 13 providers.
DR DNASU; 217370; -.
DR Ensembl; ENSMUST00000038709; ENSMUSP00000042277; ENSMUSG00000039294.
DR Ensembl; ENSMUST00000106115; ENSMUSP00000101721; ENSMUSG00000039294.
DR GeneID; 217370; -.
DR KEGG; mmu:217370; -.
DR UCSC; uc007mvn.2; mouse.
DR CTD; 79415; -.
DR MGI; MGI:2384959; Cybc1.
DR VEuPathDB; HostDB:ENSMUSG00000039294; -.
DR eggNOG; ENOG502S06T; Eukaryota.
DR GeneTree; ENSGT00390000004691; -.
DR InParanoid; Q3TYS2; -.
DR OMA; IRSWSLC; -.
DR OrthoDB; 1293240at2759; -.
DR PhylomeDB; Q3TYS2; -.
DR TreeFam; TF332389; -.
DR BioGRID-ORCS; 217370; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Cybc1; mouse.
DR PRO; PR:Q3TYS2; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q3TYS2; protein.
DR Bgee; ENSMUSG00000039294; Expressed in granulocyte and 232 other tissues.
DR ExpressionAtlas; Q3TYS2; baseline and differential.
DR Genevisible; Q3TYS2; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IMP:UniProtKB.
DR GO; GO:0045728; P:respiratory burst after phagocytosis; IMP:UniProtKB.
DR InterPro; IPR027846; Cybc1.
DR PANTHER; PTHR31837; PTHR31837; 1.
DR Pfam; PF15169; Cybc1_Eros; 1.
PE 1: Evidence at protein level;
KW Chaperone; Endoplasmic reticulum; Immunity; Innate immunity; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..187
FT /note="Cytochrome b-245 chaperone 1"
FT /id="PRO_0000281419"
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 167..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 168
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AYA6"
FT MOD_RES 170
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AYA6"
FT CONFLICT 111
FT /note="N -> S (in Ref. 1; BAE34490)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 187 AA; 20921 MW; 2158A8FB1591C0E0 CRC64;
MYMQVETRTS TRLHLKRAPG IRSWSLLVGI LSTGLAAAYY SGDSLGWKLF YVTGCLFVAV
QNLEDWEEAI FNKNTGKVIL KTFSLYKKLL TLLRAGHDQV VVLLKDIQDV NVEEEKVRYF
GKGYMVVLRF ATGFSHPLTQ SAVMGRRSDV EAIAKLITSF LELHRLESPS ERSQSSDSEP
DGPGGQS
