CXA5_MOUSE
ID CXA5_MOUSE Reviewed; 358 AA.
AC Q01231;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Gap junction alpha-5 protein;
DE AltName: Full=Connexin-40;
DE Short=Cx40;
GN Name=Gja5; Synonyms=Cxn-40;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=1318884; DOI=10.1083/jcb.117.6.1299;
RA Hennemann J., Suchyna T., Lichtenberg-Frate H., Jungbluth S., Dahl E.,
RA Schwarz J., Nicholson B.J., Willecke K.;
RT "Molecular cloning and functional expression of mouse connexin40, a second
RT gap junction gene preferentially expressed in lung.";
RL J. Cell Biol. 117:1299-1310(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: One gap junction consists of a cluster of closely packed
CC pairs of transmembrane channels, the connexons, through which materials
CC of low MW diffuse from one cell to a neighboring cell.
CC -!- SUBUNIT: A connexon is composed of a hexamer of connexins.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell
CC junction, gap junction.
CC -!- TISSUE SPECIFICITY: Kidney, heart and skin, but most abundant in lung.
CC -!- SIMILARITY: Belongs to the connexin family. Alpha-type (group II)
CC subfamily. {ECO:0000305}.
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DR EMBL; X61675; CAA43850.1; -; Genomic_DNA.
DR EMBL; BC053054; AAH53054.1; -; mRNA.
DR CCDS; CCDS17652.1; -.
DR PIR; S23111; S23111.
DR RefSeq; NP_001258557.1; NM_001271628.1.
DR RefSeq; NP_032147.1; NM_008121.3.
DR AlphaFoldDB; Q01231; -.
DR SMR; Q01231; -.
DR STRING; 10090.ENSMUSP00000088264; -.
DR iPTMnet; Q01231; -.
DR PhosphoSitePlus; Q01231; -.
DR MaxQB; Q01231; -.
DR PaxDb; Q01231; -.
DR PRIDE; Q01231; -.
DR ProteomicsDB; 284072; -.
DR Antibodypedia; 72405; 299 antibodies from 31 providers.
DR DNASU; 14613; -.
DR Ensembl; ENSMUST00000072600; ENSMUSP00000088264; ENSMUSG00000057123.
DR GeneID; 14613; -.
DR KEGG; mmu:14613; -.
DR UCSC; uc008qoo.2; mouse.
DR CTD; 2702; -.
DR MGI; MGI:95716; Gja5.
DR VEuPathDB; HostDB:ENSMUSG00000057123; -.
DR eggNOG; ENOG502QW11; Eukaryota.
DR GeneTree; ENSGT01050000244864; -.
DR HOGENOM; CLU_037388_0_0_1; -.
DR InParanoid; Q01231; -.
DR OMA; MASHPFN; -.
DR OrthoDB; 992551at2759; -.
DR PhylomeDB; Q01231; -.
DR TreeFam; TF329606; -.
DR Reactome; R-MMU-190861; Gap junction assembly.
DR BioGRID-ORCS; 14613; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Gja5; mouse.
DR PRO; PR:Q01231; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q01231; protein.
DR Bgee; ENSMUSG00000057123; Expressed in intersomitic artery and 98 other tissues.
DR ExpressionAtlas; Q01231; baseline and differential.
DR Genevisible; Q01231; MM.
DR GO; GO:0042995; C:cell projection; ISO:MGI.
DR GO; GO:0005922; C:connexin complex; ISO:MGI.
DR GO; GO:0005921; C:gap junction; IDA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:MGI.
DR GO; GO:0014704; C:intercalated disc; ISO:MGI.
DR GO; GO:0071253; F:connexin binding; ISO:MGI.
DR GO; GO:0097718; F:disordered domain specific binding; ISO:MGI.
DR GO; GO:0005243; F:gap junction channel activity; ISO:MGI.
DR GO; GO:0086076; F:gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling; ISO:MGI.
DR GO; GO:0086077; F:gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling; ISO:MGI.
DR GO; GO:0086078; F:gap junction channel activity involved in bundle of His cell-Purkinje myocyte electrical coupling; IMP:BHF-UCL.
DR GO; GO:0086075; F:gap junction channel activity involved in cardiac conduction electrical coupling; ISO:MGI.
DR GO; GO:0055077; F:gap junction hemi-channel activity; ISO:MGI.
DR GO; GO:0048844; P:artery morphogenesis; IMP:BHF-UCL.
DR GO; GO:0086044; P:atrial cardiac muscle cell to AV node cell communication by electrical coupling; IMP:BHF-UCL.
DR GO; GO:0003283; P:atrial septum development; ISO:MGI.
DR GO; GO:0060413; P:atrial septum morphogenesis; IMP:MGI.
DR GO; GO:0003294; P:atrial ventricular junction remodeling; IMP:MGI.
DR GO; GO:0086067; P:AV node cell to bundle of His cell communication; IMP:MGI.
DR GO; GO:0086053; P:AV node cell to bundle of His cell communication by electrical coupling; IMP:BHF-UCL.
DR GO; GO:0001568; P:blood vessel development; IMP:MGI.
DR GO; GO:0097746; P:blood vessel diameter maintenance; IMP:MGI.
DR GO; GO:0086054; P:bundle of His cell to Purkinje myocyte communication by electrical coupling; IMP:BHF-UCL.
DR GO; GO:0061337; P:cardiac conduction; IMP:MGI.
DR GO; GO:0003161; P:cardiac conduction system development; IMP:MGI.
DR GO; GO:0010643; P:cell communication by chemical coupling; ISO:MGI.
DR GO; GO:0010644; P:cell communication by electrical coupling; ISO:MGI.
DR GO; GO:0086064; P:cell communication by electrical coupling involved in cardiac conduction; IMP:MGI.
DR GO; GO:0086065; P:cell communication involved in cardiac conduction; IMP:MGI.
DR GO; GO:0007267; P:cell-cell signaling; IBA:GO_Central.
DR GO; GO:0035050; P:embryonic heart tube development; IMP:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:MGI.
DR GO; GO:0035922; P:foramen ovale closure; IMP:MGI.
DR GO; GO:0016264; P:gap junction assembly; ISO:MGI.
DR GO; GO:0003174; P:mitral valve development; ISO:MGI.
DR GO; GO:0045776; P:negative regulation of blood pressure; ISO:MGI.
DR GO; GO:0003105; P:negative regulation of glomerular filtration; ISO:MGI.
DR GO; GO:0003151; P:outflow tract morphogenesis; ISO:MGI.
DR GO; GO:0010652; P:positive regulation of cell communication by chemical coupling; ISO:MGI.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; ISO:MGI.
DR GO; GO:0006813; P:potassium ion transport; ISO:MGI.
DR GO; GO:0003193; P:pulmonary valve formation; ISO:MGI.
DR GO; GO:0098910; P:regulation of atrial cardiac muscle cell action potential; ISO:MGI.
DR GO; GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; IMP:MGI.
DR GO; GO:0098904; P:regulation of AV node cell action potential; IMP:BHF-UCL.
DR GO; GO:0098905; P:regulation of bundle of His cell action potential; IMP:BHF-UCL.
DR GO; GO:0055117; P:regulation of cardiac muscle contraction; ISO:MGI.
DR GO; GO:0010649; P:regulation of cell communication by electrical coupling; ISO:MGI.
DR GO; GO:0086091; P:regulation of heart rate by cardiac conduction; IMP:MGI.
DR GO; GO:1900825; P:regulation of membrane depolarization during cardiac muscle cell action potential; IMP:MGI.
DR GO; GO:0098906; P:regulation of Purkinje myocyte action potential; ISO:MGI.
DR GO; GO:1900133; P:regulation of renin secretion into blood stream; IMP:MGI.
DR GO; GO:0003073; P:regulation of systemic arterial blood pressure; IMP:MGI.
DR GO; GO:0060373; P:regulation of ventricular cardiac muscle cell membrane depolarization; IMP:BHF-UCL.
DR GO; GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; IMP:BHF-UCL.
DR GO; GO:0003071; P:renal system process involved in regulation of systemic arterial blood pressure; IMP:MGI.
DR GO; GO:0086015; P:SA node cell action potential; IMP:MGI.
DR GO; GO:0003284; P:septum primum development; IMP:MGI.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR GO; GO:0042311; P:vasodilation; ISO:MGI.
DR GO; GO:1990029; P:vasomotion; ISO:MGI.
DR GO; GO:0086005; P:ventricular cardiac muscle cell action potential; IMP:MGI.
DR GO; GO:0003281; P:ventricular septum development; ISO:MGI.
DR GO; GO:0060412; P:ventricular septum morphogenesis; IMP:MGI.
DR DisProt; DP00646; -.
DR Gene3D; 1.20.1440.80; -; 1.
DR InterPro; IPR000500; Connexin.
DR InterPro; IPR002264; Connexin40.
DR InterPro; IPR034634; Connexin_C.
DR InterPro; IPR019570; Connexin_CCC.
DR InterPro; IPR017990; Connexin_CS.
DR InterPro; IPR013092; Connexin_N.
DR InterPro; IPR038359; Connexin_N_sf.
DR InterPro; IPR031862; Cx40_C.
DR PANTHER; PTHR11984; PTHR11984; 1.
DR PANTHER; PTHR11984:SF13; PTHR11984:SF13; 1.
DR Pfam; PF00029; Connexin; 1.
DR Pfam; PF16791; Connexin40_C; 1.
DR PRINTS; PR00206; CONNEXIN.
DR PRINTS; PR01135; CONNEXINA5.
DR SMART; SM00037; CNX; 1.
DR SMART; SM01089; Connexin_CCC; 1.
DR SUPFAM; SSF118220; SSF118220; 1.
DR PROSITE; PS00407; CONNEXINS_1; 1.
DR PROSITE; PS00408; CONNEXINS_2; 1.
PE 2: Evidence at transcript level;
KW Cell junction; Cell membrane; Gap junction; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..358
FT /note="Gap junction alpha-5 protein"
FT /id="PRO_0000057820"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..76
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 186..205
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..358
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 242..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 318..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 353
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P28234"
FT MOD_RES 357
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P28234"
SQ SEQUENCE 358 AA; 40413 MW; C5418F657142896A CRC64;
MGDWSFLGEF LEEVHKHSTV IGKVWLTVLF IFRMLVLGTA AESSWGDEQA DFRCDTIQPG
CQNVCYDQAF PISHIRYWVL QIIFVSTPSL VYMGHAMHTV RMQEKQKLRD AEKAKEAHRT
GAYEYPVAEK AELSCWKEVD GKIVLQGTLL NTYVCTILIR TTMEVAFIVG QYLLYGIFLD
TLHVCRRSPC PHPVNCYVSR PTEKNVFIVF MMAVAGLSLF LSLAELYHLG WKKIRQRFGK
SRQGVDKHQL PGPPTSLVQS LTPPPDFNQC LKNSSGEKFF SDFSNNMGSR KNPDALATGE
VPNQEQIPGE GFIHMHYSQK PEYASGASAG HRLPQGYHSD KRRLSKASSK ARSDDLSV
