CUPP_DROME
ID CUPP_DROME Reviewed; 184 AA.
AC P14484; A8WHK2; Q9VM52;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Pupal cuticle protein;
DE Flags: Precursor;
GN Name=Pcp; ORFNames=CG3440;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3079672; DOI=10.1016/0092-8674(86)90482-4;
RA Henikoff S., Keene M.A., Fechtel K., Fristrom J.W.;
RT "Gene within a gene: nested Drosophila genes encode unrelated proteins on
RT opposite DNA strands.";
RL Cell 44:33-42(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=3123310; DOI=10.1093/genetics/117.4.711;
RA Henikoff S., Eghtedarzadeh M.K.;
RT "Conserved arrangement of nested genes at the Drosophila Gart locus.";
RL Genetics 117:711-725(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the cuticle of the pupa of fruit fly.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout both the fourth and the fifth
CC larval instars.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABX00770.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; J02527; AAA28564.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF52475.1; -; Genomic_DNA.
DR EMBL; BT031148; ABX00770.1; ALT_INIT; mRNA.
DR PIR; B24787; UCFFPM.
DR RefSeq; NP_476673.1; NM_057325.3.
DR AlphaFoldDB; P14484; -.
DR STRING; 7227.FBpp0079016; -.
DR PaxDb; P14484; -.
DR DNASU; 33985; -.
DR EnsemblMetazoa; FBtr0079388; FBpp0079016; FBgn0003046.
DR GeneID; 33985; -.
DR KEGG; dme:Dmel_CG3440; -.
DR CTD; 18543; -.
DR FlyBase; FBgn0003046; Pcp.
DR VEuPathDB; VectorBase:FBgn0003046; -.
DR eggNOG; ENOG502T8G8; Eukaryota.
DR GeneTree; ENSGT00900000141325; -.
DR HOGENOM; CLU_065450_0_0_1; -.
DR InParanoid; P14484; -.
DR OMA; IRTHPYQ; -.
DR OrthoDB; 1459720at2759; -.
DR PhylomeDB; P14484; -.
DR BioGRID-ORCS; 33985; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33985; -.
DR PRO; PR:P14484; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0003046; Expressed in saliva-secreting gland and 11 other tissues.
DR Genevisible; P14484; DM.
DR GO; GO:0062129; C:chitin-based extracellular matrix; ISM:FlyBase.
DR GO; GO:0008010; F:structural constituent of chitin-based larval cuticle; ISM:FlyBase.
DR GO; GO:0008011; F:structural constituent of pupal chitin-based cuticle; ISS:FlyBase.
DR GO; GO:0040003; P:chitin-based cuticle development; ISM:FlyBase.
DR InterPro; IPR031311; CHIT_BIND_RR_consensus.
DR InterPro; IPR000618; Insect_cuticle.
DR Pfam; PF00379; Chitin_bind_4; 1.
DR PRINTS; PR00947; CUTICLE.
DR PROSITE; PS00233; CHIT_BIND_RR_1; 1.
DR PROSITE; PS51155; CHIT_BIND_RR_2; 1.
PE 2: Evidence at transcript level;
KW Cuticle; Reference proteome; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT CHAIN 16..184
FT /note="Pupal cuticle protein"
FT /id="PRO_0000006397"
FT DOMAIN 42..103
FT /note="Chitin-binding type R&R"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00497"
FT REGION 147..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..178
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 4
FT /note="L -> LQ (in Ref. 1; AAA28564)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 184 AA; 20633 MW; 4493B893F61329C2 CRC64;
MYLLVNFIVA LAVLQVQAGS SYIPDSDRNT RTLQNDLQVE RDGKYRYAYE TSNGISASQE
GLGGVAVQGG SSYTSPEGEV ISVNYVADEF GYHPVGAHIP QVPDYILRSL EYIRTHPYQI
KDYYTGELKT VEHDAAAFNV YTRNIQDHTI PQSRPSTTPK TIYLTHPPTT TSRPLRQRRA
LPTH
