ID   CUH1C_TENMO             Reviewed;         211 AA.
AC   P80686; O18509;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Larval/pupal cuticle protein H1C;
DE   AltName: Full=TM-PCP H1C;
DE            Short=TM-H1C;
DE   AltName: Full=TMLPCP-22;
DE   Flags: Precursor;
GN   Name=LPCP-22;
OS   Tenebrio molitor (Yellow mealworm beetle).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia;
OC   Tenebrionidae; Tenebrio.
OX   NCBI_TaxID=7067;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Epidermis;
RX   PubMed=8631320; DOI=10.1111/j.1432-1033.1996.00138.x;
RA   Rondot I., Quennedey B., Courrent A., Lemoine A., Delachambre J.;
RT   "Cloning and sequencing of a cDNA encoding a larval-pupal-specific
RT   cuticular protein in Tenebrio molitor (Insecta, Coleoptera). Developmental
RT   expression and effect of a juvenile hormone analogue.";
RL   Eur. J. Biochem. 235:138-143(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9660184; DOI=10.1046/j.1432-1327.1998.2540304.x;
RA   Rondot I., Quennedey B., Delachambre J.;
RT   "Structure, organization and expression of two clustered cuticle protein
RT   genes during the metamorphosis of an insect, Tenebrio molitor.";
RL   Eur. J. Biochem. 254:304-312(1998).
RN   [3]
RP   PROTEIN SEQUENCE OF 17-211, AND MASS SPECTROMETRY.
RC   TISSUE=Cuticle;
RX   PubMed=9066122; DOI=10.1016/s0965-1748(96)00076-8;
RA   Andersen S.O., Rafn K., Roepstorff P.;
RT   "Sequence studies of proteins from larval and pupal cuticle of the yellow
RT   meal worm, Tenebrio molitor.";
RL   Insect Biochem. Mol. Biol. 27:121-131(1997).
CC   -!- FUNCTION: Component of the hard and soft cuticles of the larva and pupa
CC       of Tenebrio molitor.
CC   -!- TISSUE SPECIFICITY: Restricted to sternal, tergal, legs and wing
CC       anlagen epidermis, and soft cuticle secreting epidermis, such as
CC       intersegmental membranes and pleura. No expression in muscles or fat
CC       body.
CC   -!- DEVELOPMENTAL STAGE: Expressed in epidermal cells during the secretion
CC       of preecdysial larval and pupal cuticles. Restricted to a short part of
CC       the intersegmental regions after pupal ecdysis.
CC   -!- INDUCTION: By juvenile hormone. {ECO:0000305}.
CC   -!- DOMAIN: The tetrapeptide (A-A-P-[AV]) repeats found throughout the
CC       protein are also present in many proteins constituting the protective
CC       envelope of other species.
CC   -!- MASS SPECTROMETRY: Mass=19182.7; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9066122};
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DR   EMBL; X87983; CAA61238.1; -; mRNA.
DR   EMBL; Y09124; CAA70342.1; -; Genomic_DNA.
DR   PIR; S62598; S62598.
DR   PIR; T12013; T12013.
DR   AlphaFoldDB; P80686; -.
DR   GO; GO:0042302; F:structural constituent of cuticle; IEA:UniProtKB-KW.
DR   InterPro; IPR022727; Cuticle_C1.
DR   PANTHER; PTHR39068; PTHR39068; 1.
DR   Pfam; PF11018; Cuticle_3; 2.
PE   1: Evidence at protein level;
KW   Cuticle; Direct protein sequencing; Repeat; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000269|PubMed:9066122"
FT   CHAIN           17..211
FT                   /note="Larval/pupal cuticle protein H1C"
FT                   /id="PRO_0000006417"
FT   REPEAT          29..32
FT                   /note="1-1"
FT   REPEAT          41..44
FT                   /note="1-2"
FT   REPEAT          128..137
FT                   /note="2-1"
FT   REPEAT          131..134
FT                   /note="1-3"
FT   REPEAT          138..147
FT                   /note="2-2"
FT   REPEAT          141..144
FT                   /note="1-4"
FT   REPEAT          148..156
FT                   /note="3-1"
FT   REPEAT          157..165
FT                   /note="3-2"
FT   REPEAT          166..174
FT                   /note="3-3"
FT   REPEAT          175..183
FT                   /note="3-4"
FT   REGION          128..147
FT                   /note="2 X 10 AA tandem repeat of Y-A-H-A-A-P-V-V-S-[ST]"
FT   REGION          148..183
FT                   /note="4 X 9 AA tandem repeat of Y-A-A-H-A-P-V-[VA]-[AT]"
FT   CONFLICT        156
FT                   /note="A -> T (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        181
FT                   /note="V -> VATYAAHAPV (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   211 AA;  20913 MW;  2E21FAE609F4FE37 CRC64;
     MYKFVVFAAA LAYANAGLIG APAVAAYSAA PAVSSAYIHQ AAPVAVAHAA PLAVAHAPVA
     VAHAAPYAIH APAVGASHQS VVRSLGGNQA VSHYSKAVDS AFSSVRKFDT RVTNDALAVA
     HAPVVSTYAH AAPVVSTYAH AAPVVSSYAA HAPVAAYAAH APVATYAAHA PVATYAAHAP
     VVATRTSAVA YSPAAVVSHA SFSGLGASYA W