CST9_MOUSE
ID CST9_MOUSE Reviewed; 137 AA.
AC Q9Z0H6; Q545L7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cystatin-9;
DE AltName: Full=Testatin {ECO:0000303|PubMed:9826679};
DE Flags: Precursor;
GN Name=Cst9; Synonyms=Cresp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC TISSUE=Testis;
RX PubMed=9826679; DOI=10.1073/pnas.95.24.14208;
RA Toehoenen V., Oesterlund C., Nordqvist K.;
RT "Testatin: a cystatin-related gene expressed during early testis
RT development.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:14208-14213(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kanno Y., Tamura M., Nakatuji N.;
RT "Identification of Cresp, a novel cystatin-related gene.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=15923608; DOI=10.1128/mcb.25.12.4892-4902.2005;
RA Toehoenen V., Frygelius J., Mohammadieh M., Kvist U., Pelliniemi L.J.,
RA O'Brien K., Nordqvist K., Wedell A.;
RT "Normal sexual development and fertility in testatin knockout mice.";
RL Mol. Cell. Biol. 25:4892-4902(2005).
RN [7]
RP FUNCTION.
RX PubMed=23922243; DOI=10.2119/molmed.2013.00081;
RA Eaves-Pyles T., Patel J., Arigi E., Cong Y., Cao A., Garg N., Dhiman M.,
RA Pyles R.B., Arulanandam B., Miller A.L., Popov V.L., Soong L.,
RA Carlsen E.D., Coletta C., Szabo C., Almeida I.C.;
RT "Immunomodulatory and antibacterial effects of cystatin 9 against
RT Francisella tularensis.";
RL Mol. Med. 19:263-275(2013).
CC -!- FUNCTION: May be involved in testis development (PubMed:9826679). May
CC play a role in hematopoietic differentiation or inflammation (By
CC similarity). Has immunomodulatory and antimicrobial functions against
CC Francisella tularensis, a Gram-negative bacteria (PubMed:23922243).
CC {ECO:0000250|UniProtKB:Q5W186, ECO:0000269|PubMed:23922243,
CC ECO:0000305|PubMed:9826679}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expression is restricted to fetal gonads and adult
CC testis. {ECO:0000269|PubMed:9826679}.
CC -!- DEVELOPMENTAL STAGE: Expressed during testis cord formation in pre-
CC Sertoli cells, at a time immediately after the peak of SRY expression.
CC {ECO:0000269|PubMed:9826679}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype; probably due to redundancy
CC with other family members. {ECO:0000269|PubMed:15923608}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; Y18243; CAA77090.1; -; mRNA.
DR EMBL; AB017157; BAA37089.1; -; mRNA.
DR EMBL; AK006429; BAB24584.1; -; mRNA.
DR EMBL; AL844519; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC048486; AAH48486.1; -; mRNA.
DR CCDS; CCDS16850.1; -.
DR RefSeq; NP_034109.1; NM_009979.1.
DR AlphaFoldDB; Q9Z0H6; -.
DR SMR; Q9Z0H6; -.
DR STRING; 10090.ENSMUSP00000028935; -.
DR GlyGen; Q9Z0H6; 2 sites.
DR PhosphoSitePlus; Q9Z0H6; -.
DR PaxDb; Q9Z0H6; -.
DR PRIDE; Q9Z0H6; -.
DR ProteomicsDB; 283969; -.
DR DNASU; 13013; -.
DR Ensembl; ENSMUST00000028935; ENSMUSP00000028935; ENSMUSG00000027445.
DR GeneID; 13013; -.
DR KEGG; mmu:13013; -.
DR UCSC; uc008mtr.1; mouse.
DR CTD; 128822; -.
DR MGI; MGI:1340053; Cst9.
DR VEuPathDB; HostDB:ENSMUSG00000027445; -.
DR eggNOG; ENOG502TAJ0; Eukaryota.
DR GeneTree; ENSGT00940000161877; -.
DR HOGENOM; CLU_118168_3_1_1; -.
DR InParanoid; Q9Z0H6; -.
DR OMA; HIFNLQN; -.
DR OrthoDB; 1577727at2759; -.
DR PhylomeDB; Q9Z0H6; -.
DR BioGRID-ORCS; 13013; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q9Z0H6; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9Z0H6; protein.
DR Bgee; ENSMUSG00000027445; Expressed in seminiferous tubule of testis and 22 other tissues.
DR Genevisible; Q9Z0H6; MM.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0019730; P:antimicrobial humoral response; IDA:UniProtKB.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR043250; CST9-like.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR PANTHER; PTHR46945; PTHR46945; 1.
DR Pfam; PF00031; Cystatin; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Disulfide bond; Glycoprotein; Protease inhibitor;
KW Reference proteome; Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..137
FT /note="Cystatin-9"
FT /id="PRO_0000006657"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 92..102
FT /evidence="ECO:0000250"
FT DISULFID 116..136
FT /evidence="ECO:0000250"
SQ SEQUENCE 137 AA; 16094 MW; AC1CDE5834C36FB7 CRC64;
MSCPLRKKAL PLTMLLLLLS FHVLITPVSK ANKETNRSVH FIPTVEFAVN TFNQESQDEY
AYRMEHIMSS WREKVNFPTV YSMRLQLRRT ICKKFEESLD ICPFQESHGL NNTFTCLFTV
GTYPWITKFK LFRSVCS
