CRN2_PHYIN
ID CRN2_PHYIN Reviewed; 456 AA.
AC Q8H6Z4;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=Crinkler effector protein 2 {ECO:0000303|PubMed:12840044};
DE Flags: Precursor;
GN Name=CRN2 {ECO:0000303|PubMed:12840044};
OS Phytophthora infestans (Potato late blight agent) (Botrytis infestans).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=4787;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND FUNCTION.
RC STRAIN=DDR7602;
RX PubMed=12840044; DOI=10.1101/gr.910003;
RA Torto T.A., Li S., Styer A., Huitema E., Testa A., Gow N.A., van West P.,
RA Kamoun S.;
RT "EST mining and functional expression assays identify extracellular
RT effector proteins from the plant pathogen Phytophthora.";
RL Genome Res. 13:1675-1685(2003).
RN [2]
RP DOMAIN, AND FUNCTION.
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [3]
RP DOMAIN, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=20847293; DOI=10.1073/pnas.1008491107;
RA Schornack S., van Damme M., Bozkurt T.O., Cano L.M., Smoker M., Thines M.,
RA Gaulin E., Kamoun S., Huitema E.;
RT "Ancient class of translocated oomycete effectors targets the host
RT nucleus.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:17421-17426(2010).
CC -!- FUNCTION: Secreted effector that effector that induces cell death when
CC expressed in host plants (PubMed:19741609, PubMed:20847293). Induces
CC the expression of defense response genes in tomato (PubMed:12840044).
CC {ECO:0000269|PubMed:12840044, ECO:0000269|PubMed:19741609,
CC ECO:0000269|PubMed:20847293}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20847293}. Host
CC nucleus {ECO:0000269|PubMed:20847293}.
CC -!- INDUCTION: Expressed during colonization of the host plant tomato by
CC P.infestans. {ECO:0000269|PubMed:12840044}.
CC -!- DOMAIN: The CRN proteins have modular architectures that include a
CC signal peptide, a conserved N-terminus, and highly diverse C-terminal
CC domains. The conserved CRN N-terminus harbors a distinct LXLFLAK motif,
CC which is followed by the conserved DWL domain. A highly conserved
CC HVLVXXP motif marks the end of the CRN N-terminal domains and forms a
CC junction where diverse C-terminal domains are fused. The conserved CRN
CC N-terminus mediates the translocation into the plant host cells.
CC {ECO:0000269|PubMed:20847293}.
CC -!- SIMILARITY: Belongs to the Crinkler effector family. {ECO:0000305}.
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DR EMBL; AF424677; AAN31502.1; -; mRNA.
DR AlphaFoldDB; Q8H6Z4; -.
DR VEuPathDB; FungiDB:PITG_17199; -.
DR PHI-base; PHI:657; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0001907; P:killing by symbiont of host cells; IDA:PAMGO_VMD.
DR InterPro; IPR045379; Crinkler_N.
DR Pfam; PF20147; Crinkler; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Host nucleus; Secreted; Signal; Virulence.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..456
FT /note="Crinkler effector protein 2"
FT /id="PRO_0000447407"
FT REGION 18..54
FT /note="LQLFLAK domain"
FT /evidence="ECO:0000305|PubMed:20847293"
FT REGION 55..136
FT /note="DWL domain"
FT /evidence="ECO:0000305|PubMed:20847293"
FT MOTIF 137..143
FT /note="HVLVXXP motif"
FT /evidence="ECO:0000305|PubMed:20847293"
FT CARBOHYD 338
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 456 AA; 50215 MW; FC75416BAB8F7C3F CRC64;
MVKLVCAIVG VAGSAFPVDT DASQLVGDLK KAIKAENAMT FTGDAKDLQL FLAKQPVDDE
SGKEVVPVYR PSAEEMKEES FKWLPDEHRA ALKLVEGESD DYIHALTAGE PILGSKTLTT
WFYTKNNMEL PSSEQIHVLV VVPDGAGGSA SDTSRMDRLF DKVDKVYEHT VLSKRTRYVH
SEMNSAKGNI LLNDLKIRIS PVDTVKFAGG VPTPAKEFKW KSDRTEEQQK EPYREYVVAN
IGDVLTNNKL CVVGVEKGVN ILTVEVPGRD IVLAGRTDMI VLSDIAQKFP HYLPHLPGVR
MLIEVKKVVT TASEFQALSE LIALDIIATE SVMALLTNLT NHWQFFWVSR KSDDRVIIET
TTLIAPGEAF AVIRTLLDQS PSAGAEVSLP CFEKPVKRQK LSQLLPSISE ASGSSGIRES
IERYYDIASM LGPDLEMARA VASQVARSIP TLSYFS
