COX8B_RAT
ID COX8B_RAT Reviewed; 70 AA.
AC P16221;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Cytochrome c oxidase subunit 8B, mitochondrial;
DE AltName: Full=Cytochrome c oxidase polypeptide VIII-heart;
DE AltName: Full=Cytochrome c oxidase subunit 8-1;
DE AltName: Full=Cytochrome c oxidase subunit 8H;
DE Flags: Precursor;
GN Name=Cox8b; Synonyms=Cox8h;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Heart;
RX PubMed=1324738; DOI=10.1016/0167-4781(92)90059-9;
RA Scheja K., Kadenbach B.;
RT "Nucleotide sequence of cDNA encoding subunit VIII of cytochrome c oxidase
RT from rat heart.";
RL Biochim. Biophys. Acta 1132:91-93(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Fischer;
RX PubMed=8939982; DOI=10.1074/jbc.271.47.30281;
RA Lenka N., Basu A., Mullick J., Avadhani N.G.;
RT "The role of an E box binding basic helix loop helix protein in the cardiac
RT muscle-specific expression of the rat cytochrome oxidase subunit VIII
RT gene.";
RL J. Biol. Chem. 271:30281-30289(1996).
RN [3]
RP PROTEIN SEQUENCE OF 25-62.
RC TISSUE=Heart;
RX PubMed=2153407; DOI=10.1016/0005-2728(90)90042-3;
RA Kadenbach B., Stroh A., Becker A., Eckersorn C., Lottspeich F.;
RT "Tissue- and species-specific expression of cytochrome c oxidase isozymes
RT in vertebrates.";
RL Biochim. Biophys. Acta 1015:368-372(1990).
RN [4]
RP PROTEIN SEQUENCE OF 25-39.
RC TISSUE=Heart;
RX PubMed=7601105; DOI=10.1111/j.1432-1033.1995.tb20556.x;
RA Schaegger H., Noack H., Halangk W., Brandt U., von Jagow G.;
RT "Cytochrome-c oxidase in developing rat heart. Enzymic properties and
RT amino-terminal sequences suggest identity of the fetal heart and the adult
RT liver isoform.";
RL Eur. J. Biochem. 230:235-241(1995).
CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the
CC mitochondrial electron transport chain which drives oxidative
CC phosphorylation. The respiratory chain contains 3 multisubunit
CC complexes succinate dehydrogenase (complex II, CII), ubiquinol-
CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III,
CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to
CC transfer electrons derived from NADH and succinate to molecular oxygen,
CC creating an electrochemical gradient over the inner membrane that
CC drives transmembrane transport and the ATP synthase. Cytochrome c
CC oxidase is the component of the respiratory chain that catalyzes the
CC reduction of oxygen to water. Electrons originating from reduced
CC cytochrome c in the intermembrane space (IMS) are transferred via the
CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1
CC to the active site in subunit 1, a binuclear center (BNC) formed by
CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2
CC water molecules using 4 electrons from cytochrome c in the IMS and 4
CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P10175}.
CC -!- PATHWAY: Energy metabolism; oxidative phosphorylation.
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a
CC multisubunit enzyme composed of 14 subunits. The complex is composed of
CC a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in
CC the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A,
CC COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which
CC are encoded in the nuclear genome. The complex exists as a monomer or a
CC dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex
CC III, CIII), resulting in different assemblies (supercomplex
CC SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P10175}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P10175}.
CC -!- DEVELOPMENTAL STAGE: Increased levels in liver during prenatal
CC development.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase VIII family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X64827; CAA46039.1; -; mRNA.
DR EMBL; U40836; AAC52906.1; -; Genomic_DNA.
DR EMBL; U40835; AAC52906.1; JOINED; Genomic_DNA.
DR PIR; S24171; S24171.
DR RefSeq; NP_036918.2; NM_012786.3.
DR AlphaFoldDB; P16221; -.
DR SMR; P16221; -.
DR STRING; 10116.ENSRNOP00000019679; -.
DR PaxDb; P16221; -.
DR GeneID; 25250; -.
DR KEGG; rno:25250; -.
DR UCSC; RGD:2386; rat.
DR CTD; 12869; -.
DR RGD; 2386; Cox8b.
DR eggNOG; ENOG502SB3F; Eukaryota.
DR HOGENOM; CLU_203368_0_0_1; -.
DR InParanoid; P16221; -.
DR OMA; AGWVLYH; -.
DR OrthoDB; 1632471at2759; -.
DR PhylomeDB; P16221; -.
DR TreeFam; TF105070; -.
DR UniPathway; UPA00705; -.
DR PRO; PR:P16221; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000014656; Expressed in heart and 20 other tissues.
DR Genevisible; P16221; RN.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IEA:InterPro.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0045277; C:respiratory chain complex IV; IBA:GO_Central.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; TAS:RGD.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; TAS:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR Gene3D; 4.10.81.10; -; 1.
DR InterPro; IPR003205; Cyt_c_oxidase_su8.
DR InterPro; IPR036548; Cyt_c_oxidase_su8_sf.
DR PANTHER; PTHR16717; PTHR16717; 1.
DR Pfam; PF02285; COX8; 1.
DR SUPFAM; SSF81431; SSF81431; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..24
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:2153407,
FT ECO:0000269|PubMed:7601105"
FT CHAIN 25..70
FT /note="Cytochrome c oxidase subunit 8B, mitochondrial"
FT /id="PRO_0000006179"
FT TOPO_DOM 25..35
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT TRANSMEM 36..59
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT TOPO_DOM 60..70
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:P10175"
FT CONFLICT 47
FT /note="I -> V (in Ref. 1; CAA46039)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 70 AA; 7549 MW; 9DF87F37FB7D2D51 CRC64;
MPRLPPILRL LQAPEKYTVI PKARISSKPA KSPTSAMDQA VGMSVIIAGF MVPAGWVLSH
LESYKRSSAA
