ID   COPB_SHIFL              Reviewed;          86 AA.
AC   P62537; P03855;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Replication regulatory protein repA2;
DE   AltName: Full=Protein CopB;
GN   Name=repA2; Synonyms=copB, repB; OrderedLocusNames=CP0258;
OS   Shigella flexneri.
OG   Plasmid pWR100, Plasmid pCP301, and Plasmid pINV_F6_M1382.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX   PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA   Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA   Kunst F., Sansonetti P.J., Parsot C.;
RT   "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT   the type III secretion apparatus of Shigella flexneri.";
RL   Mol. Microbiol. 38:760-771(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX   PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA   Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA   Blattner F.R.;
RT   "Complete DNA sequence and analysis of the large virulence plasmid of
RT   Shigella flexneri.";
RL   Infect. Immun. 69:3271-3285(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M1382 / Serotype 6; PLASMID=pINV_F6_M1382;
RX   PubMed=14573649; DOI=10.1128/iai.71.11.6298-6306.2003;
RA   Lan R., Stevenson G., Reeves P.R.;
RT   "Comparison of two major forms of the Shigella virulence plasmid pINV:
RT   positive selection is a major force driving the divergence.";
RL   Infect. Immun. 71:6298-6306(2003).
CC   -!- FUNCTION: This protein is involved in the determination of copy number
CC       in gene replication. It binds to the repA promoter thus inhibiting the
CC       synthesis of the mRNA for the initiator protein repA (By similarity).
CC       {ECO:0000250}.
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DR   EMBL; AL391753; CAC05867.1; -; Genomic_DNA.
DR   EMBL; AF348706; AAK18589.1; -; Genomic_DNA.
DR   EMBL; AF386526; AAL72557.1; -; Genomic_DNA.
DR   EMBL; AY206448; AAP79039.1; -; Genomic_DNA.
DR   RefSeq; NP_085433.1; NC_002698.1.
DR   RefSeq; NP_858391.1; NC_004851.1.
DR   RefSeq; WP_000083819.1; NZ_WPGW01000206.1.
DR   RefSeq; YP_006960282.1; NC_019197.1.
DR   RefSeq; YP_009062549.1; NC_024996.1.
DR   AlphaFoldDB; P62537; -.
DR   SMR; P62537; -.
DR   STRING; 198214.CP0258; -.
DR   EnsemblBacteria; AAL72557; AAL72557; SF_p0258.
DR   GeneID; 1238260; -.
DR   KEGG; sfl:CP0258; -.
DR   PATRIC; fig|198214.7.peg.5520; -.
DR   HOGENOM; CLU_180887_0_0_6; -.
DR   OMA; RERKMRS; -.
DR   PRO; PR:P62537; -.
DR   Proteomes; UP000001006; Plasmid pCP301.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR   InterPro; IPR019661; RepA2.
DR   Pfam; PF10723; RepB-RCR_reg; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Plasmid; Plasmid copy control; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..86
FT                   /note="Replication regulatory protein repA2"
FT                   /id="PRO_0000068294"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   86 AA;  10038 MW;  F7AD85B2C211CBF8 CRC64;
     MSQTENAVTS SLSQKRFVRR GKPMTDSEKQ MAAVARKRLT HKEIKVFVKN PLKDLMVEYC
     EREGITQAQF VEKIIKDELQ RLDILK