COPB2_ECOLX
ID COPB2_ECOLX Reviewed; 84 AA.
AC P03847; P13947;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Replication regulatory protein repA2;
DE AltName: Full=Protein CopB;
GN Name=repA2; Synonyms=copB;
OS Escherichia coli.
OG Plasmid IncFII R100 (NR1), Plasmid R6-5, and Plasmid IncFVI pSU212.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=7003300; DOI=10.1007/bf00271742;
RA Rosen J., Ryder T., Inokuchi H., Ohtsubo H., Ohtsubo E.;
RT "Genes and sites involved in replication and incompatibility of an R100
RT plasmid derivative based on nucleotide sequence analysis.";
RL Mol. Gen. Genet. 179:527-537(1980).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=3019092; DOI=10.1016/0065-227x(86)90018-3;
RA Ohtsubo H., Ryder T.B., Maeda Y., Armstrong K., Ohtsubo E.;
RT "DNA replication of the resistance plasmid R100 and its control.";
RL Adv. Biophys. 21:115-133(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=R6-5;
RX PubMed=7029525; DOI=10.1073/pnas.78.8.4699;
RA Danbara H., Brady G., Timmis J.K., Timmis K.N.;
RT "Regulation of DNA replication: 'target' determinant of the replication
RT control elements of plasmid R6-5 lies within a control element gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 78:4699-4703(1981).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=2445727; DOI=10.1128/jb.169.12.5353-5363.1987;
RA Dong X.N., Womble D.D., Rownd R.H.;
RT "Transcriptional pausing in a region important for plasmid NR1 replication
RT control.";
RL J. Bacteriol. 169:5353-5363(1987).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=2580099; DOI=10.1016/0022-2836(85)90228-1;
RA Womble D.D., Sampathkumar P., Easton A.M., Luckow V.A., Rownd R.H.;
RT "Transcription of the replication control region of the IncFII R-plasmid
RT NR1 in vitro and in vivo.";
RL J. Mol. Biol. 181:395-410(1985).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFVI pSU212;
RX PubMed=1865183; DOI=10.1099/00221287-137-5-1093;
RA Lopez J., Delgado D., Andres I., Ortiz J.M., Rodriguez J.C.;
RT "Isolation and evolutionary analysis of a RepFVIB replicon of the plasmid
RT pSU212.";
RL J. Gen. Microbiol. 137:1093-1099(1991).
CC -!- FUNCTION: This protein is involved in the determination of copy number
CC in gene replication. It binds to the repA promoter thus inhibiting the
CC synthesis of the mRNA for the initiator protein RepA.
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DR EMBL; J01762; AAA92255.1; -; Genomic_DNA.
DR EMBL; M26840; AAA26065.1; -; Genomic_DNA.
DR EMBL; V00318; CAA23608.1; -; Genomic_DNA.
DR EMBL; M18273; AAA88332.1; -; Genomic_DNA.
DR EMBL; X02302; CAA26165.1; -; Genomic_DNA.
DR EMBL; X55895; CAA39380.1; -; Genomic_DNA.
DR PIR; A04476; QQECAR.
DR PIR; I51821; I51821.
DR RefSeq; NP_957641.1; NC_005327.1.
DR RefSeq; WP_000083830.1; NZ_WVVN01000083.1.
DR RefSeq; YP_001096509.1; NC_009133.1.
DR RefSeq; YP_002456233.1; NC_011812.1.
DR RefSeq; YP_003108214.1; NC_013121.1.
DR RefSeq; YP_003108323.1; NC_013122.1.
DR RefSeq; YP_004869996.1; NC_016039.1.
DR RefSeq; YP_006952182.1; NC_019057.1.
DR RefSeq; YP_006953265.1; NC_019071.1.
DR RefSeq; YP_006953363.1; NC_019072.1.
DR RefSeq; YP_006953800.1; NC_019089.1.
DR RefSeq; YP_006953885.1; NC_019090.1.
DR RefSeq; YP_006954159.1; NC_019094.1.
DR RefSeq; YP_006954221.1; NC_019095.1.
DR RefSeq; YP_006990697.1; NC_019424.1.
DR RefSeq; YP_007447499.1; NC_020278.2.
DR RefSeq; YP_009066513.1; NC_025106.1.
DR AlphaFoldDB; P03847; -.
DR SMR; P03847; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR InterPro; IPR019661; RepA2.
DR Pfam; PF10723; RepB-RCR_reg; 1.
PE 4: Predicted;
KW DNA-binding; Plasmid; Plasmid copy control; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..84
FT /note="Replication regulatory protein repA2"
FT /id="PRO_0000068291"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 3
FT /note="Q -> H (in Ref. 2; AAA26065)"
FT /evidence="ECO:0000305"
FT CONFLICT 77..84
FT /note="SEAQKRCM -> K (in Ref. 2; AAA26065)"
FT /evidence="ECO:0000305"
FT CONFLICT 81..84
FT /note="KRCM -> NDACDDGLTFLSVQKISARLLIV (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 84 AA; 9438 MW; ADC3442D128E2C81 CRC64;
MSQTENAVTS SSGAKRAYRK GNPLSDAEKQ RLSVARKRAS FKEVKVFLEP KYKAMLMQMC
HEDGLTQAEV LTALIKSEAQ KRCM
