COPB2_DROME
ID COPB2_DROME Reviewed; 914 AA.
AC O62621; Q3KN33; Q8MRM3; Q9VJZ0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Coatomer subunit beta';
DE AltName: Full=Beta'-coat protein;
DE Short=Beta'-COP;
GN Name=beta'COP {ECO:0000312|FlyBase:FBgn0025724};
GN ORFNames=CG6699 {ECO:0000312|FlyBase:FBgn0025724};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC TISSUE=Embryo;
RA Merdes G., Heid H.W., Mechler B.M.;
RT "Cloning and characterization of the Drosophila coatomer subunit beta'.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 613-914.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC dilysine motifs and reversibly associates with Golgi non-clathrin-
CC coated vesicles, which further mediate biosynthetic protein transport
CC from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC complex is required for budding from Golgi membranes, and is essential
CC for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Cytoplasmic vesicle, COPI-coated vesicle membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=The coatomer is cytoplasmic or polymerized on
CC the cytoplasmic side of the Golgi, as well as on the vesicles/buds
CC originating from it. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat COPB2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM50181.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ006523; CAA07084.1; -; mRNA.
DR EMBL; AJ006524; CAA07085.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF53294.2; -; Genomic_DNA.
DR EMBL; BT023906; ABA81840.1; -; mRNA.
DR EMBL; AY119527; AAM50181.1; ALT_INIT; mRNA.
DR RefSeq; NP_524836.2; NM_080097.4.
DR AlphaFoldDB; O62621; -.
DR SMR; O62621; -.
DR BioGRID; 69859; 15.
DR DIP; DIP-22262N; -.
DR IntAct; O62621; 3.
DR STRING; 7227.FBpp0080048; -.
DR PaxDb; O62621; -.
DR PRIDE; O62621; -.
DR DNASU; 45757; -.
DR EnsemblMetazoa; FBtr0080469; FBpp0080048; FBgn0025724.
DR GeneID; 45757; -.
DR KEGG; dme:Dmel_CG6699; -.
DR CTD; 45757; -.
DR FlyBase; FBgn0025724; beta'COP.
DR VEuPathDB; VectorBase:FBgn0025724; -.
DR eggNOG; KOG0276; Eukaryota.
DR GeneTree; ENSGT00900000141083; -.
DR HOGENOM; CLU_005507_1_0_1; -.
DR InParanoid; O62621; -.
DR OMA; NVFWNES; -.
DR OrthoDB; 139008at2759; -.
DR PhylomeDB; O62621; -.
DR Reactome; R-DME-6807878; COPI-mediated anterograde transport.
DR Reactome; R-DME-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR SignaLink; O62621; -.
DR BioGRID-ORCS; 45757; 1 hit in 1 CRISPR screen.
DR ChiTaRS; beta'COP; fly.
DR GenomeRNAi; 45757; -.
DR PRO; PR:O62621; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0025724; Expressed in spermathecum and 25 other tissues.
DR Genevisible; O62621; DM.
DR GO; GO:0030126; C:COPI vesicle coat; IDA:FlyBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005795; C:Golgi stack; IDA:FlyBase.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0010883; P:regulation of lipid storage; IDA:FlyBase.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; ISS:FlyBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR InterPro; IPR016453; COPB2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF04053; Coatomer_WDAD; 1.
DR Pfam; PF00400; WD40; 4.
DR PIRSF; PIRSF005567; Coatomer_beta'_subunit; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus;
KW Membrane; Protein transport; Reference proteome; Repeat; Transport;
KW WD repeat.
FT CHAIN 1..914
FT /note="Coatomer subunit beta'"
FT /id="PRO_0000050916"
FT REPEAT 13..54
FT /note="WD 1"
FT REPEAT 55..94
FT /note="WD 2"
FT REPEAT 97..136
FT /note="WD 3"
FT REPEAT 140..180
FT /note="WD 4"
FT REPEAT 183..224
FT /note="WD 5"
FT REPEAT 227..266
FT /note="WD 6"
FT REPEAT 352..390
FT /note="WD 7"
FT CONFLICT 254
FT /note="R -> C (in Ref. 1; CAA07084/CAA07085)"
FT /evidence="ECO:0000305"
FT CONFLICT 343..345
FT /note="LPV -> CPF (in Ref. 1; CAA07084/CAA07085)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 914 AA; 102713 MW; 15E862E1944F5C4B CRC64;
MPLKLDIKRR LTSRSDRVKC VDLHPAEPWM LCALYNGHVH IMNYENQQMV KDFEVCDVPV
RSARFVARKN WILTGSDDMQ IRVFNYNTLE KVHSFEAHSD YLRCIAVHPT QPLVLTSSDD
MLIKLWNWEK MWACQRVFEG HTHYVMQIVF NPKDNNTFAS ASLDRTVKVW QLGSNFANFT
LEGHEKGVNC VDYYHGGDKP YLISGADDRL VKIWDYQNKT CVQTLEGHAQ NISAVCFHPE
LPIVLTGSED GTVRIWHSGT YRLETCLNYG FERVWTISSM RGTNNVALGY DEGSIIIKVG
REEPAMSMDV VGSKIIWAKH SEMQQVNLKT IADGTEIKDG ERLPVATKDM GACEIYPQTI
AHNPNGRFVV VCGDGEYIIY TSMALRNKAF GSAQEFVWAL ESNEYAIREN NGTVRLFRNF
KERKSFTPEY GAESIYGGYY FGVKTSSGLA FYDWETLQLV RRIEVQPKNV FWNESGSLVC
LATDDSYFVL GVDTAQVANA VETKEGLEDD GVESAFNVLG EVSECVKTGL WVGDCFIYTN
SVNRINYYVG GEIVTVSHLD RTMYLLGYVP KDNRIYLGDK ELNVISFCLQ LSVLEYQTAV
MRRDFERADV VLPTIPKEHR TRVAHFLEKQ GFKSQALQVS TDADHKFDLA LQIGDLEIAL
KLARESENSQ KWSQLADVAS SKNNMSLVKE CMQKANDLSG LLLLSTASGD AQLLEVVGAA
GSAQGHHNLA FLSAFLRSDV ERCLEILIET NRLPEAAFFA RTYLPSQMSR IVELWREKLG
KVNEKAGQSL ADPAQYTNLF PGLGDALRVE QHLQEERARK APARLAANLP LNSERHPLQE
LFAAEQGAAG QHLEEKVKPA YVPAQAVVSS SQVAEPTSAA DDDDDLDLEI DGITLDDNID
TTDVNLDDDF LSDD
