ID   COPB2_DICDI             Reviewed;        1005 AA.
AC   Q54YD8;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Coatomer subunit beta';
DE   AltName: Full=Beta'-coat protein;
DE            Short=Beta'-COP;
GN   Name=copb2; ORFNames=DDB_G0278285;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC       dilysine motifs and reversibly associates with Golgi non-clathrin-
CC       coated vesicles, which further mediate biosynthetic protein transport
CC       from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC       complex is required for budding from Golgi membranes, and is essential
CC       for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC       beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Cytoplasmic vesicle, COPI-coated vesicle membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=The coatomer is cytoplasmic or polymerized on
CC       the cytoplasmic side of the Golgi, as well as on the vesicles/buds
CC       originating from it. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat COPB2 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000023; EAL68315.1; -; Genomic_DNA.
DR   RefSeq; XP_642264.1; XM_637172.1.
DR   AlphaFoldDB; Q54YD8; -.
DR   SMR; Q54YD8; -.
DR   STRING; 44689.DDB0233798; -.
DR   PaxDb; Q54YD8; -.
DR   EnsemblProtists; EAL68315; EAL68315; DDB_G0278285.
DR   GeneID; 8621473; -.
DR   KEGG; ddi:DDB_G0278285; -.
DR   dictyBase; DDB_G0278285; copB2.
DR   eggNOG; KOG0276; Eukaryota.
DR   HOGENOM; CLU_005507_1_0_1; -.
DR   InParanoid; Q54YD8; -.
DR   OMA; NVFWNES; -.
DR   PhylomeDB; Q54YD8; -.
DR   Reactome; R-DDI-6807878; COPI-mediated anterograde transport.
DR   Reactome; R-DDI-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR   PRO; PR:Q54YD8; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0030126; C:COPI vesicle coat; ISS:dictyBase.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR   InterPro; IPR016453; COPB2.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF04053; Coatomer_WDAD; 1.
DR   Pfam; PF00400; WD40; 6.
DR   PIRSF; PIRSF005567; Coatomer_beta'_subunit; 1.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; SSF50978; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport;
KW   Golgi apparatus; Membrane; Protein transport; Reference proteome; Repeat;
KW   Transport; WD repeat.
FT   CHAIN           1..1005
FT                   /note="Coatomer subunit beta'"
FT                   /id="PRO_0000330864"
FT   REPEAT          13..54
FT                   /note="WD 1"
FT   REPEAT          56..95
FT                   /note="WD 2"
FT   REPEAT          98..137
FT                   /note="WD 3"
FT   REPEAT          141..181
FT                   /note="WD 4"
FT   REPEAT          184..225
FT                   /note="WD 5"
FT   REPEAT          228..267
FT                   /note="WD 6"
FT   REPEAT          349..389
FT                   /note="WD 7"
FT   REPEAT          391..426
FT                   /note="WD 8"
FT   REGION          908..948
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          973..1005
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          829..853
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        974..990
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1005 AA;  113222 MW;  0758B8E35000054F CRC64;
     MPLRLDIKKK LSTRSDRVKS VDIHPTEPWI LASLYDGNVY IWNYETQNMV KSFEVSPNNP
     VRTARFIAKK QWIVTGSDDT YIRVYNYNTM EKIKSFEAHA DYIRCIIVHP TLPYILSSSD
     DMFIKLWDYE KGWSNTQVFE GHSHYVMSIA WNPKDTNQFA TASLDKTVKV WSINSPHPHF
     TLEGHEKGIN SVEYFSGGEK PYLISGADDK LVKIWDYQSK TCVQTLEGHS NNVSVVCYHP
     ELPLILSGSE DGTVKLWHSS TYRLERTLNY GMGFVWSMNF LRGSNFIGLG YDDGTVVLKI
     GKNKPPVSMD QGGKVIYAKH NEIRISNISS TLEQEVQDGE KLSLQSKDLG NCEVFPQSLQ
     HNSNGRFVSV CGDGEFIIYT ALAWRNKSFG NALEFVWAED SGQYAVREST SRIKIFKNFK
     ETHSFKPSFS AEGIFGGSLL GVKSNDTLCF YSWDSADIIR RIEKTSPKNI FWSENGDYLA
     IVTDKSTFIL RYYKDTVQKY IESGQPIGEL GIENAFDVVH EIEDTIGTGL WVGDCFIYIN
     RSSKLNYCVG TEVVTISHLE KHMYLLKYLP QSGRLYLSDK NLNIVSYKLH ISVISYQTSI
     LRGDLEGAER ILPKIPQDQR NSIAHFLESQ GYKEKALEVS TDLDHRFELA IQLENLDVAH
     EIALKSDSET KFKHLGDLAL SIGEIKLAEN CLKKAEDLPG LLLLYTSTGD IEGMKMLAKL
     SEEKGQTNIS FICNLLLPNS LLNCLNTLCA SGDYSEAAFM ARTYLPSMVP DMVEKWKESL
     KSTSSKASDA LSNPIDFPNL FPQYDLSIKA EKYFESLRSQ PKSSFDYLKN ATAQSLSKKQ
     RNLLEEIESV SQQDYDKVLS QLQHFSLHSG ISDITTTTTN TNNNTNNTNN NNTASLLDDL
     DFTSTTTTTT TTTNTNTTQK VASPLSSPSP IRKNDSIASP QKQQQQQQDI NITAPPLIDP
     SPVDHDLVLE PTPIINTVSP TKTSPTADLS FLDNIPVDHD EEDEF