ID   COBQ_STRSV              Reviewed;         499 AA.
AC   A3CL74;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=SSA_0481;
OS   Streptococcus sanguinis (strain SK36).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=388919;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SK36;
RX   PubMed=17277061; DOI=10.1128/jb.01808-06;
RA   Xu P., Alves J.M., Kitten T., Brown A., Chen Z., Ozaki L.S., Manque P.,
RA   Ge X., Serrano M.G., Puiu D., Hendricks S., Wang Y., Chaplin M.D., Akan D.,
RA   Paik S., Peterson D.L., Macrina F.L., Buck G.A.;
RT   "Genome of the opportunistic pathogen Streptococcus sanguinis.";
RL   J. Bacteriol. 189:3166-3175(2007).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; CP000387; ABN43929.1; -; Genomic_DNA.
DR   RefSeq; WP_011836536.1; NC_009009.1.
DR   RefSeq; YP_001034479.1; NC_009009.1.
DR   AlphaFoldDB; A3CL74; -.
DR   SMR; A3CL74; -.
DR   STRING; 388919.SSA_0481; -.
DR   PRIDE; A3CL74; -.
DR   EnsemblBacteria; ABN43929; ABN43929; SSA_0481.
DR   KEGG; ssa:SSA_0481; -.
DR   PATRIC; fig|388919.9.peg.465; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_9; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002148; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..499
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_1000002379"
FT   DOMAIN          251..442
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        332
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        434
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   499 AA;  55586 MW;  401D5D847DD47AF0 CRC64;
     MVKALMVQGT ASDAGKSIIA AGLCRIFKQD GLEVVPFKSQ NMALNSFITK KGDEMGRAQV
     VQAEAAGKEP DVRMNPVLLK PTSDRKSQVV FLGRVLRDMD AVEYHEYKQQ LLPKIKEVYD
     ELGAENDIIV IEGAGSPAEI NLNDRDIVNM GMAKLVDAPV ILVADIDKGG VFASIYGTIE
     LMPPEDRKRI KGVIINKFRG DVALLQSGID MIEELTQVPV IGVVPYAQLD IDSEDSVALV
     QKSRRFDSRK SLDIAVVSLK RLSNFTDFHS LEIQPDVSVR YVQPGDAIGR PDLLILPGSK
     NTIEDMNYLC ESGLEAEILE CLEQGVRIFG ICGGYQLLGQ KISDPLHLES DLEETRGLGI
     LETETVLQPV KRTTQVRALH EGQELEGYEI HMGETQLADR LEPFSIIKEQ NGEATERPDG
     AVAYGGQVQG TYLHGVFDNL EWTRQYLNEL RLAKGLEPLE DQLVSIKDFK DREYDKLADV
     LRQSLDMEQI YQIINREEK