ACOB_CUPNH
ID ACOB_CUPNH Reviewed; 338 AA.
AC P27746; Q0K4X3;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit beta;
DE Short=Acetoin:DCPIP oxidoreductase-beta;
DE Short=Ao:DCPIP OR;
DE EC=1.1.1.-;
DE AltName: Full=TPP-dependent acetoin dehydrogenase E1 subunit beta;
GN Name=acoB; OrderedLocusNames=H16_B0145;
OS Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS / H16 / Stanier 337) (Ralstonia eutropha).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=381666;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-23.
RX PubMed=2061286; DOI=10.1128/jb.173.13.4056-4071.1991;
RA Priefert H., Hein S., Krueger N., Zeh K., Schmidt B., Steinbuechel A.;
RT "Identification and molecular characterization of the Alcaligenes eutrophus
RT H16 aco operon genes involved in acetoin catabolism.";
RL J. Bacteriol. 173:4056-4071(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX PubMed=16964242; DOI=10.1038/nbt1244;
RA Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT eutropha H16.";
RL Nat. Biotechnol. 24:1257-1262(2006).
CC -!- FUNCTION: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage
CC of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is
CC probably not the catalytic subunit of the enzyme.
CC -!- PATHWAY: Ketone degradation; acetoin degradation.
CC -!- SUBUNIT: Tetramer of 2 alpha and 2 beta subunits.
CC -!- INDUCTION: By growth on acetoin.
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DR EMBL; M66060; AAA21949.1; -; Genomic_DNA.
DR EMBL; AM260480; CAJ94951.1; -; Genomic_DNA.
DR PIR; C42462; C42462.
DR RefSeq; WP_010813529.1; NZ_CP039288.1.
DR AlphaFoldDB; P27746; -.
DR SMR; P27746; -.
DR STRING; 381666.H16_B0145; -.
DR EnsemblBacteria; CAJ94951; CAJ94951; H16_B0145.
DR GeneID; 57646024; -.
DR KEGG; reh:H16_B0145; -.
DR eggNOG; COG0022; Bacteria.
DR HOGENOM; CLU_012907_1_0_4; -.
DR OMA; HSQMLTP; -.
DR OrthoDB; 900125at2; -.
DR BRENDA; 2.3.1.190; 11493.
DR UniPathway; UPA00040; -.
DR Proteomes; UP000008210; Chromosome 2.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0045150; P:acetoin catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.920; -; 1.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR009014; Transketo_C/PFOR_II.
DR InterPro; IPR005475; Transketolase-like_Pyr-bd.
DR InterPro; IPR033248; Transketolase_C.
DR Pfam; PF02779; Transket_pyr; 1.
DR Pfam; PF02780; Transketolase_C; 1.
DR SMART; SM00861; Transket_pyr; 1.
DR SUPFAM; SSF52518; SSF52518; 1.
DR SUPFAM; SSF52922; SSF52922; 1.
PE 1: Evidence at protein level;
KW Acetoin catabolism; Direct protein sequencing; Oxidoreductase;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2061286"
FT CHAIN 2..338
FT /note="Acetoin:2,6-dichlorophenolindophenol oxidoreductase
FT subunit beta"
FT /id="PRO_0000162308"
SQ SEQUENCE 338 AA; 35960 MW; 023FFDA0E47BDEFB CRC64;
MARKLSIKLA INEAIDQEMT RDPSVIMLGE DIVGGAGADG EKDAWGGVLG VTKGLYAKHG
DRLLDTPLSE SAYVGAAIGA AACGMRPIAE LMFIDFMGVC FDQIFNQAAK FRYMFGGKAE
TPVVIRAMVG AGFRAAAQHS QMLTPLFTHI PGLKVVCPST PYDTKGLLIQ AIRDNDPVIF
CEHKNLYGLE GEVPEGAYAI PFGEANIVRD GKDVSIVTYG LMVHRALEAA ATLAKEGIEA
EIVDLRTLSP LDMDTVLESV ENTGRLVVVD EASPRCNIAT DISAQVAQQA FGALKAGIEM
VCPPHTPVPF SPTLEDLYIP SAAQIAAAAR KTMKGGKH
