ID   ACNR_CORDI              Reviewed;         190 AA.
AC   Q6NH62;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=HTH-type transcriptional repressor AcnR {ECO:0000250|UniProtKB:Q8NQ97};
GN   Name=acnR {ECO:0000250|UniProtKB:Q8NQ97}; OrderedLocusNames=DIP1284;
OS   Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype
OS   gravis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium.
OX   NCBI_TaxID=257309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700971 / NCTC 13129 / Biotype gravis;
RX   PubMed=14602910; DOI=10.1093/nar/gkg874;
RA   Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G.,
RA   Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D.,
RA   De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N.,
RA   Holroyd S., Jagels K., Moule S., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G.,
RA   Parkhill J.;
RT   "The complete genome sequence and analysis of Corynebacterium diphtheriae
RT   NCTC13129.";
RL   Nucleic Acids Res. 31:6516-6523(2003).
CC   -!- FUNCTION: AcnR negatively controls the expression of the aconitase gene
CC       acn. {ECO:0000250|UniProtKB:Q8NQ97}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q8NQ97}.
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DR   EMBL; BX248357; CAE49811.1; -; Genomic_DNA.
DR   RefSeq; WP_010934950.1; NC_002935.2.
DR   AlphaFoldDB; Q6NH62; -.
DR   SMR; Q6NH62; -.
DR   STRING; 257309.DIP1284; -.
DR   EnsemblBacteria; CAE49811; CAE49811; DIP1284.
DR   GeneID; 29422804; -.
DR   KEGG; cdi:DIP1284; -.
DR   HOGENOM; CLU_069356_15_12_11; -.
DR   OMA; PEFSRGW; -.
DR   OrthoDB; 2047542at2; -.
DR   Proteomes; UP000002198; Chromosome.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Magnesium; Metal-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..190
FT                   /note="HTH-type transcriptional repressor AcnR"
FT                   /id="PRO_0000070569"
FT   DOMAIN          10..70
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        33..52
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   BINDING         79..80
FT                   /ligand="citrate"
FT                   /ligand_id="ChEBI:CHEBI:16947"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NQ97"
FT   BINDING         130
FT                   /ligand="citrate"
FT                   /ligand_id="ChEBI:CHEBI:16947"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NQ97"
FT   BINDING         134
FT                   /ligand="citrate"
FT                   /ligand_id="ChEBI:CHEBI:16947"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NQ97"
FT   BINDING         181
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NQ97"
FT   BINDING         185
FT                   /ligand="citrate"
FT                   /ligand_id="ChEBI:CHEBI:16947"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NQ97"
SQ   SEQUENCE   190 AA;  21831 MW;  849460E6FAB4E6CE CRC64;
     MPVVSNTELS MRRQEILEGA RRCFAEHGYE GATVRRLEET VGKSRGAIFH HFSDKENLFL
     ALAREDAARM AEVVAENGLV EVMRDMLAHP ERHDWLATRL EITKMLRTDP SFRNRWIEHQ
     RVLDDAVLER LQRNAHLGRM RDDVPIDVLH TYLETVLDGF ISRLASGGDT DNLERVLDLV
     EESVRSSQPS