ACM4_MOUSE
ID ACM4_MOUSE Reviewed; 479 AA.
AC P32211; Q64056;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Muscarinic acetylcholine receptor M4;
DE AltName: Full=Mm4 mAChR;
GN Name=Chrm4; Synonyms=Chrm-4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7916637; DOI=10.1016/0167-4781(93)90135-z;
RA van Koppen C.J., Lenz W., Nathanson N.M.;
RT "Isolation, sequence and functional expression of the mouse m4 muscarinic
RT acetylcholine receptor gene.";
RL Biochim. Biophys. Acta 1173:342-344(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 235-355.
RC TISSUE=Brain;
RX PubMed=7874308; DOI=10.1111/j.1460-9568.1994.tb00561.x;
RA Andre C., Dos Santos G., Koulakoff A.;
RT "Cultured neurons from mouse brain reproduce the muscarinic receptor
RT profile of their tissue of origin.";
RL Eur. J. Neurosci. 6:1691-1701(1994).
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is
CC inhibition of adenylate cyclase.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Postsynaptic cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; X63473; CAA45071.1; -; Genomic_DNA.
DR EMBL; S74916; AAB33577.1; -; mRNA.
DR CCDS; CCDS16439.1; -.
DR PIR; S33776; S33776.
DR RefSeq; NP_031725.1; NM_007699.2.
DR AlphaFoldDB; P32211; -.
DR SMR; P32211; -.
DR IntAct; P32211; 1.
DR MINT; P32211; -.
DR STRING; 10090.ENSMUSP00000040808; -.
DR BindingDB; P32211; -.
DR ChEMBL; CHEMBL4572; -.
DR DrugCentral; P32211; -.
DR GlyGen; P32211; 2 sites.
DR iPTMnet; P32211; -.
DR PhosphoSitePlus; P32211; -.
DR SwissPalm; P32211; -.
DR PaxDb; P32211; -.
DR PRIDE; P32211; -.
DR ProteomicsDB; 285645; -.
DR Antibodypedia; 26327; 208 antibodies from 30 providers.
DR DNASU; 12672; -.
DR Ensembl; ENSMUST00000045537; ENSMUSP00000040808; ENSMUSG00000040495.
DR GeneID; 12672; -.
DR KEGG; mmu:12672; -.
DR UCSC; uc008kwv.2; mouse.
DR CTD; 1132; -.
DR MGI; MGI:88399; Chrm4.
DR VEuPathDB; HostDB:ENSMUSG00000040495; -.
DR eggNOG; KOG4220; Eukaryota.
DR GeneTree; ENSGT00940000160394; -.
DR HOGENOM; CLU_009579_11_2_1; -.
DR InParanoid; P32211; -.
DR OMA; YKTVEMV; -.
DR OrthoDB; 1245472at2759; -.
DR PhylomeDB; P32211; -.
DR TreeFam; TF320495; -.
DR Reactome; R-MMU-390648; Muscarinic acetylcholine receptors.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR BioGRID-ORCS; 12672; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Chrm4; mouse.
DR PRO; PR:P32211; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P32211; protein.
DR Bgee; ENSMUSG00000040495; Expressed in superior frontal gyrus and 59 other tissues.
DR ExpressionAtlas; P32211; baseline and differential.
DR Genevisible; P32211; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0032279; C:asymmetric synapse; ISO:MGI.
DR GO; GO:0043679; C:axon terminus; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; ISO:MGI.
DR GO; GO:0099056; C:integral component of presynaptic membrane; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0014069; C:postsynaptic density; ISO:MGI.
DR GO; GO:0042383; C:sarcolemma; ISO:MGI.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; IDA:MGI.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISO:MGI.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; IDA:MGI.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0040012; P:regulation of locomotion; IEA:InterPro.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001432; Musac_Ach_M4_rcpt.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR PANTHER; PTHR24248:SF142; PTHR24248:SF142; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PRINTS; PR00541; MUSCRINICM4R.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Synapse; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..479
FT /note="Muscarinic acetylcholine receptor M4"
FT /id="PRO_0000069038"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 31..53
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 54..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 89..105
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 106..127
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 128..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 148..170
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 171..192
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 193..215
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 216..401
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 402..422
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 423..436
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 437..456
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 457..479
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 271..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..334
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 459
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 463
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 477
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 104..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 322..323
FT /note="AL -> V (in Ref. 2; AAB33577)"
FT /evidence="ECO:0000305"
FT CONFLICT 351
FT /note="S -> N (in Ref. 2; AAB33577)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 479 AA; 52973 MW; 04F301E78814CD70 CRC64;
MANFTPVNGS SANQSVRLVT TAHNHLETVE MVFIATVTGS LSLVTVVGNI LVMLSIKVNR
QLQTVNNYFL FSLACADLII GAFSMNLYTL YIIKGYWPLG AVVCDLWLAL DYVVSNASVM
NLLIISFDRY FCVTKPLTYP ARRTTKMAGL MIAAAWVLSF VLWAPAILFW QFVVGKRTVP
DNQCFIQFLS NPAVTFGTAI AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT
LAFLKSPLMK PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVADKD TSNESSSGSA
TQNTKERPPT ELSTTEAATT PALPAPTLQP RTLNPASKWS KIQIVTKQTG SECVTAIEIV
PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR TIFAILLAFI LTWTPYNVMV
LVNTFCQSCI PERVWSIGYW LCYVNSTINP ACYALCNATF KKTFRHLLLC QYRNIGTAR
