ID   CLPA_BRANA              Reviewed;         874 AA.
AC   P46523;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=ATP-dependent Clp protease ATP-binding subunit ClpA homolog, chloroplastic;
DE   Flags: Precursor; Fragment;
GN   Name=CLPA;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Topas; TISSUE=Seed;
RX   PubMed=8165250; DOI=10.1104/pp.104.3.1087;
RA   Ko K., Duong C., Ko Z.W.;
RT   "Nucleotide sequence of a Brassica napus Clp homolog.";
RL   Plant Physiol. 104:1087-1089(1994).
CC   -!- FUNCTION: May interact with a ClpP-like protease involved in
CC       degradation of denatured proteins in the chloroplast.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the ClpA/ClpB family. {ECO:0000305}.
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DR   EMBL; X75328; CAA53077.1; -; mRNA.
DR   PIR; S37557; S37557.
DR   AlphaFoldDB; P46523; -.
DR   SMR; P46523; -.
DR   MEROPS; X20.001; -.
DR   PRIDE; P46523; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   Gene3D; 1.10.1780.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR019489; Clp_ATPase_C.
DR   InterPro; IPR036628; Clp_N_dom_sf.
DR   InterPro; IPR004176; Clp_R_dom.
DR   InterPro; IPR001270; ClpA/B.
DR   InterPro; IPR018368; ClpA/B_CS1.
DR   InterPro; IPR028299; ClpA/B_CS2.
DR   InterPro; IPR041546; ClpA/ClpB_AAA_lid.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF07724; AAA_2; 1.
DR   Pfam; PF17871; AAA_lid_9; 1.
DR   Pfam; PF02861; Clp_N; 2.
DR   Pfam; PF10431; ClpB_D2-small; 1.
DR   PRINTS; PR00300; CLPPROTEASEA.
DR   SMART; SM00382; AAA; 2.
DR   SMART; SM01086; ClpB_D2-small; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF81923; SSF81923; 1.
DR   PROSITE; PS51903; CLP_R; 1.
DR   PROSITE; PS00870; CLPAB_1; 1.
DR   PROSITE; PS00871; CLPAB_2; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chaperone; Chloroplast; Nucleotide-binding; Plastid; Repeat;
KW   Transit peptide.
FT   TRANSIT         <1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..874
FT                   /note="ATP-dependent Clp protease ATP-binding subunit ClpA
FT                   homolog, chloroplastic"
FT                   /id="PRO_0000005501"
FT   DOMAIN          42..184
FT                   /note="Clp R"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   DOMAIN          458..492
FT                   /note="UVR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00217"
FT   REGION          45..110
FT                   /note="Repeat 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          120..184
FT                   /note="Repeat 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          204..451
FT                   /note="I"
FT   REGION          517..708
FT                   /note="II"
FT   REGION          852..874
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        852..868
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         249..256
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         591..598
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   874 AA;  97319 MW;  2CC673F7620C7760 CRC64;
     VAVGNNVLDT LGRSRQSFGG KVRQAMNVPK GKGSRGVVKA MFERFTEKAI KVIMLAQEEA
     RRLGHNFVGT EQILLGLIGE GTGIAAKVLK SMGINLKDAR VEVEKIIGRG SGFVAVEIPF
     TPRAKRVLEL SLEEARQLGH NYIGSEHLLL GLLREGEGVA ARVLENLGAD PSNIRTQVIR
     MVGENNEVTA NVGGGSGTNK MPTLEEYGTN LTKLAEEGKL DPVVGRHPQI ERVVQILGRR
     TKNNPCLIGE PGVGKTAIAE GLAQRIASGV VRETSEGKKV ITLDMGLLAA GTKYRGEFEE
     RVKKLMEEIK QSDEIILFID EVHTLIGAGA AEGAIDAANI LKPALARGEL QCIGATTLDE
     YRKHIEKDPA LERRFQPVKV PEPTVDETIQ ILKGLRERYE IHHKLRYTDE SLVAAAQLSY
     QYISDRFLPD RAIDLMDEAG SRVRLRHAQV PEEARELEKE LRQITKENEA VRGQDFEKAG
     TLRDREIELR AEVSAIQAKG KEMSKAESET GDEGPMVTES DIQHIVSSWT GILVEKVSTD
     ESDLLLKMEE TLHKRVIGQD EAVKAISRAI RRARVGLKNP NRPIASFIFF GPTGVGKSEL
     AKALAAYYFG CEEAMIRLDM SEFMERHTVS KLIGSPPGYV GYTEPPQLTE AVRRRPYTVV
     LFDEIEKAHP DVFNMMLQIL EDGRLTNSKG RTVDFKNTLL IMTSNVGSSV IEKGGRRIGF
     DLDYEKDSSY NRIKSLVTQE LKQYFRPEFL NRLDEMILFR QLTKLEVKEI ADILLQELFE
     RLKKKEVELQ VTERFKERVV DEGYNPSYGA RPLRRAIMRL LEDSMEEKML AREIKEGDSV
     IVDVDSEGKV TVLNGGSGTP TTSLEEQEDS LPVA