ID   CLMP1_CRYNJ             Reviewed;         472 AA.
AC   P0CP18; B0BNN5; Q55XA9; Q5KMM4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Clampless protein 1;
DE            Short=CLP1;
GN   OrderedLocusNames=CNB01190;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
RN   [2]
RP   IDENTIFICATION, FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=17993575; DOI=10.1128/ec.00377-07;
RA   Ekena J.L., Stanton B.C., Schiebe-Owens J.A., Hull C.M.;
RT   "Sexual development in Cryptococcus neoformans requires CLP1, a target of
RT   the homeodomain transcription factors Sxi1alpha and Sxi2a.";
RL   Eukaryot. Cell 7:49-57(2008).
CC   -!- FUNCTION: Required for developmental progression after cells of
CC       opposite mating types fuse with one another, essential for processes
CC       common to both dikaryotic filament formation and monokaryotic fruiting.
CC       A direct target for transcription factors Sxi1-alpha and Sxi2-a.
CC       {ECO:0000269|PubMed:17993575}.
CC   -!- DEVELOPMENTAL STAGE: Expressed abundantly under sexual development
CC       conditions. {ECO:0000269|PubMed:17993575}.
CC   -!- DISRUPTION PHENOTYPE: Expression is not seen in haploid cells where
CC       both copies of the gene are deleted and there is no discernible
CC       phenotype with respect to haplotype growth in strains with complete
CC       deletions. However, there is a complete absence of sexual development
CC       and strains fail to form dikaryotic filaments/spores. Where only a
CC       single copy is present in a wild-type and mutant mating pair sexual
CC       development is indistinguishable from a wild-type mating pair.
CC       Expression is up-regulated during sexual development.
CC       {ECO:0000269|PubMed:17993575}.
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DR   EMBL; AE017342; AAW41485.1; -; Genomic_DNA.
DR   EMBL; BK006302; DAA06128.1; -; Genomic_DNA.
DR   RefSeq; XP_568792.1; XM_568792.1.
DR   AlphaFoldDB; P0CP18; -.
DR   PaxDb; P0CP18; -.
DR   PRIDE; P0CP18; -.
DR   EnsemblFungi; AAW41485; AAW41485; CNB01190.
DR   GeneID; 3255560; -.
DR   KEGG; cne:CNB01190; -.
DR   VEuPathDB; FungiDB:CNB01190; -.
DR   eggNOG; ENOG502SCZS; Eukaryota.
DR   HOGENOM; CLU_581412_0_0_1; -.
DR   InParanoid; P0CP18; -.
DR   OMA; MATYLAP; -.
DR   OrthoDB; 1150950at2759; -.
DR   Proteomes; UP000002149; Chromosome 2.
DR   GO; GO:0000905; P:sporocarp development involved in asexual reproduction; IMP:UniProtKB.
DR   GO; GO:0000909; P:sporocarp development involved in sexual reproduction; IMP:UniProtKB.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Reference proteome.
FT   CHAIN           1..472
FT                   /note="Clampless protein 1"
FT                   /id="PRO_0000394762"
FT   CARBOHYD        70
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        296
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   472 AA;  51822 MW;  FFB240EE388D7705 CRC64;
     MATYLAPPVS SNKENSPAPN IALSDIDAIS LSLRTSLSGV TLPAKKKVAA LGLGRAPKFT
     YRRHSNKPYN RSTSITRAKK AAVQTKSVRS KAAVKKSNTR PLPLKLVQRL DVESARLERL
     RKAVWNPPAP VPGQVRVPLK LPYPRFPSIE YIDNEYLKEI PVQYIFDRMV PLLPSIATIT
     LAYQPYASIP HPDSKILRDT TLAFAIPEVI DGRKPHWAAK ARGREPDLAL AVAYKSGEGS
     NGNTVVAVNS LAFATQCAYW PRLLTTSIPI PTPKRPTPSA SAVSTSLPAI VETEENVSDA
     SFSSSSSWSD SDSEVEFIDL PRLPRPVKDD KGFLHLPLVE LPIPSPSTFP IIHRHLHHPS
     RALLPDLLGL PEHYTTRSQV LDAISGLSVQ QLMDKLTTLQ GVWQNLCSLG IGRLGTWRQL
     GEAWACVVGV IAGQGLLIAG QEEAEVQRTG RKTAAEDVAW EWVRREKAKE QQ