ID   CLML_MESEU              Reviewed;          64 AA.
AC   P86399; E4VP58;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 2.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Neurotoxin lambda-MeuTx {ECO:0000303|Ref.2};
DE   AltName: Full=Calcium channel toxin-like peptide-1;
DE   Flags: Precursor;
OS   Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34648;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA   Zhu S., Gao B.;
RT   "Molecular characterization and genomic organization of a venom calcium
RT   channel toxin-like peptide (Mevcalcinelip-1) from Mesobuthus eupeus.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 28-64, FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Venom {ECO:0000269|Ref.2};
RA   Zhu S.Y., Gao B.;
RT   "Characterization of a Kv channel blocker with inhibitor cystine knot (ICK)
RT   structural motif from the Mesobuthus eupeus venom.";
RL   Submitted (NOV-2009) to UniProtKB.
CC   -!- FUNCTION: Voltage-gated potassium channel (Kv) inhibitor (Ref.2). In
CC       addition it may increase intracellular calcium release through the
CC       activation of nuclear inositol 1,4,5-trisphosphate receptors (ITPR) of
CC       cardiomyocytes, thereby causing an increase in the contraction
CC       frequency of these cells (By similarity).
CC       {ECO:0000250|UniProtKB:P0DM29, ECO:0000269|Ref.2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.2}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.2}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P59868}.
CC   -!- SIMILARITY: Belongs to the scorpion calcin-like family. {ECO:0000305}.
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DR   EMBL; EF445100; ABR21075.1; -; mRNA.
DR   EMBL; EF445101; ABR21076.1; -; Genomic_DNA.
DR   AlphaFoldDB; P86399; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin;
KW   Potassium channel impairing toxin;
KW   Ryanodine-sensitive calcium-release channel impairing toxin; Secreted;
KW   Signal; Toxin.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   PROPEP          19..27
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000408389"
FT   PEPTIDE         28..64
FT                   /note="Neurotoxin lambda-MeuTx"
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000401130"
FT   DISULFID        29..43
FT                   /evidence="ECO:0000250|UniProtKB:P59868"
FT   DISULFID        36..49
FT                   /evidence="ECO:0000250|UniProtKB:P59868"
FT   DISULFID        42..58
FT                   /evidence="ECO:0000250|UniProtKB:P59868"
FT   CONFLICT        32
FT                   /note="L -> K (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        53
FT                   /note="G -> K (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        62
FT                   /note="F -> R (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   64 AA;  7177 MW;  889343B1FE72069F CRC64;
     MSTFIVVFLL LTAILCHAEH AIDETARGCN RLNKKCNSDA DCCRYGERCI STGVNYYCRP
     DFGP