ID   CLAPI_STYCL             Reviewed;          80 AA.
AC   O97395;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Clavaspirin;
DE   Flags: Precursor;
OS   Styela clava (Sea squirt).
OC   Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Stolidobranchia;
OC   Styelidae; Styela.
OX   NCBI_TaxID=7725;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SYNTHESIS OF 30-52, FUNCTION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Pharynx;
RX   PubMed=12005415; DOI=10.1034/j.1399-3011.2001.10974.x;
RA   Lee I.-H., Zhao C., Nguyen T., Menzel L., Waring A.J., Sherman M.A.,
RA   Lehrer R.I.;
RT   "Clavaspirin, an antibacterial and haemolytic peptide from Styela clava.";
RL   J. Pept. Res. 58:445-456(2001).
CC   -!- FUNCTION: Exhibits broad-spectrum antimicrobial activity against both
CC       Gram-positive and Gram-negative bacteria. Has potent hemolytic
CC       activity. {ECO:0000269|PubMed:12005415}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Pharyngeal tissues and hemocytes.
CC       {ECO:0000269|PubMed:12005415}.
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DR   EMBL; Y17680; CAA76812.1; -; mRNA.
DR   AlphaFoldDB; O97395; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR008453; Clavanin.
DR   Pfam; PF05452; Clavanin; 1.
PE   2: Evidence at transcript level;
KW   Amidation; Antibiotic; Antimicrobial; Cytolysis; Hemolysis; Secreted;
KW   Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   PROPEP          18..29
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000020933"
FT   PEPTIDE         30..52
FT                   /note="Clavaspirin"
FT                   /id="PRO_0000020934"
FT   PROPEP          53..80
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000020935"
FT   MOD_RES         52
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   80 AA;  8929 MW;  50E5B3C5FD77EF51 CRC64;
     MKTIILILLI LGLGIDAKSL EESKADEEKF LRFIGSVIHG IGHLVHHIGV ALGDDQQDNG
     KFYGYYAEDN GKHWYDTGDQ