CITH_KLEPN
ID CITH_KLEPN Reviewed; 444 AA.
AC P16482;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Citrate-proton symporter;
DE AltName: Full=Citrate carrier protein;
DE AltName: Full=Citrate transporter;
GN Name=citH; Synonyms=cit;
OS Klebsiella pneumoniae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13882 / NBRC 13541 / NCTC 8172;
RX PubMed=2186908; DOI=10.1111/j.1432-1033.1990.tb15502.x;
RA van der Rest M.E., Schwarz E., Oesterhelt D., Konings W.N.;
RT "DNA sequence of a citrate carrier of Klebsiella pneumoniae.";
RL Eur. J. Biochem. 189:401-407(1990).
CC -!- FUNCTION: Uptake of citrate across the boundary membrane with the
CC concomitant transport of protons into the cell (symport system).
CC {ECO:0000250|UniProtKB:P0A2G3}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- MISCELLANEOUS: Allows the utilization of citrate as a sole source of
CC carbon and energy. {ECO:0000250|UniProtKB:P0A2G3}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Metabolite:H+
CC Symporter (MHS) family (TC 2.A.1.6) family. {ECO:0000305}.
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DR EMBL; X51479; CAA35844.1; -; Genomic_DNA.
DR PIR; S09681; S09681.
DR AlphaFoldDB; P16482; -.
DR SMR; P16482; -.
DR TCDB; 2.A.1.6.1; the major facilitator superfamily (mfs).
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006101; P:citrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004736; MHS_symport.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00883; 2A0106; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 2.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Citrate utilization; Membrane; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..444
FT /note="Citrate-proton symporter"
FT /id="PRO_0000050301"
FT TOPO_DOM 1..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..72
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..137
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..177
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..251
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..289
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 311..318
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 340
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..379
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..411
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 444 AA; 48142 MW; D431F732EEDA3EC2 CRC64;
MPTARCSMRA SSTAPVRMMA TAGGARIGAI LRVTSGNFLE QFDFFLFGFY ATYIAHTFFP
ASSEFASLMM TFAVFGAGFL MRPIGAIVLG AYIDKVGRRK GLIVTLSIMA TGTFLIVLIP
SYQTIGLWAP LLVLIGRLLQ GFSAGAELGG VSVYLAEIAT PGRKGFYTSW QSGSQQVAIM
VAAAMGFALN AVLEPSAISD WGWRIPFLFG VLIVPFIFIL RRKLEETQEF TARRHHLAMR
QVFATLLANW QVVIAGMMMV AMTTTAFYLI TVYAPTFGKK VLMLSASDSL LVTLLVAISN
FFWLPVGGAL SDRFGRRSVL IAMTLLALAT AWPALTMLAN APSFLMMLSV LLWLSFIYGM
YNGAMIPALT EIMPAEVRVA GFSLAYSLAT AVFGGFTPVI STALIEYTGD KASPGYWMSF
AAICGLLATC YLYRRSAVAL QTAR
