ID   CFI_MAIZE               Reviewed;         231 AA.
AC   Q08704;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI; Synonyms=CHI1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX   PubMed=8277939; DOI=10.1007/bf00277341;
RA   Grotewold E., Peterson T.;
RT   "Isolation and characterization of a maize gene encoding chalcone flavonone
RT   isomerase.";
RL   Mol. Gen. Genet. 242:1-8(1994).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- TISSUE SPECIFICITY: Pericarp. {ECO:0000269|PubMed:8277939}.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; Z22760; CAA80441.1; -; Genomic_DNA.
DR   PIR; S41570; S41570.
DR   AlphaFoldDB; Q08704; -.
DR   SMR; Q08704; -.
DR   STRING; 4577.GRMZM2G155329_P01; -.
DR   PaxDb; Q08704; -.
DR   PRIDE; Q08704; -.
DR   MaizeGDB; 56296; -.
DR   eggNOG; ENOG502QR5P; Eukaryota.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; Q08704; baseline and differential.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase; Reference proteome.
FT   CHAIN           1..231
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000166433"
FT   BINDING         46
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         111
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         188
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            104
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   231 AA;  24250 MW;  AD64A8B04213F9F5 CRC64;
     MACRRWWSTA VVFPPVARPP GSAGSHFLGG AGVRGVEIGG NFIKFTAIGV YLEDAAVPAL
     AKKWGGKTAD ELASDAAFFR DVVTGDFEKF TRVTMILPLT GEQYAEKVTE NCVAFWKAAG
     LYTDAEGVAV EKFREVFKPE TFAPGRSILF THSPAGVLTV AFSKDSSVPA AGGVAIENKR
     LCEAVLESII GERGVSPAAK LSLAARVSEL LAKETAAAAD APQAEPVSIT A