CFC1_MOUSE
ID CFC1_MOUSE Reviewed; 202 AA.
AC P97766; Q496U5; Q9JIB7;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Cryptic protein;
DE Flags: Precursor;
GN Name=Cfc1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND GLYCOSYLATION.
RC STRAIN=129/Sv;
RX PubMed=9053319; DOI=10.1242/dev.124.2.429;
RA Shen M.M., Wang H., Leder P.;
RT "A differential display strategy identifies Cryptic, a novel EGF-related
RT gene expressed in the axial and lateral mesoderm during mouse
RT gastrulation.";
RL Development 124:429-442(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
RX PubMed=11024280; DOI=10.1016/s0378-1119(00)00337-1;
RA Colas J.-F., Schoenwolf G.C.;
RT "Subtractive hybridization identifies chick-cripto, a novel EGF-CFC
RT ortholog expressed during gastrulation, neurulation and early
RT cardiogenesis.";
RL Gene 255:205-217(2000).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10574770; DOI=10.1016/s0960-9822(00)80059-7;
RA Gaio U., Schweickert A., Fischer A., Garratt A.N., Mueller T., Oezcelik C.,
RA Lankes W., Strehle M., Britsch S., Blum M., Birchmeier C.;
RT "A role of the cryptic gene in the correct establishment of the left-right
RT axis.";
RL Curr. Biol. 9:1339-1342(1999).
CC -!- FUNCTION: Nodal coreceptor involved in the correct establishment of the
CC left-right axis. May play a role in mesoderm and/or neural patterning
CC during gastrulation. {ECO:0000269|PubMed:10574770,
CC ECO:0000269|PubMed:9053319}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-
CC anchor {ECO:0000250}. Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: No expressed in adult tissues.
CC {ECO:0000269|PubMed:9053319}.
CC -!- DEVELOPMENTAL STAGE: Expressed during gastrulation (from 6.5 dpc to 11
CC dpc) in two spatial domains that correspond to the axial and lateral
CC mesoderm. In the first domain expression is progressively localized to
CC the anterior primitive streak, the head process, and the node and
CC notochordal. In the second domain, expression is initially concentrated
CC in the lateral region of the egg cylinder, and is later found
CC circumferentially in the intermediate and lateral plate mesoderm.
CC Furthermore, the expression can also be detected at the early head-fold
CC stage in the midline neuroectoderm, and consequently is an early marker
CC for the prospective floor plate of the neural tube. Expression ceases
CC at the end of gastrulation, and has not been observed in later
CC embryonic stages. {ECO:0000269|PubMed:9053319}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:9053319}.
CC -!- DISRUPTION PHENOTYPE: Positional defects in internal organs. The lung
CC presents a right pulmonary isomerism. The stomach is located on either
CC the left or the right and the spleen is small and has an abnormal
CC shape. The apex of the heart pointed to the right or left. In addition
CC malpositioning of heart outflow tracts is observed, the aorta is
CC connected to the right ventricle and emerged from the heart in a
CC ventral position and to the right of the pulmonary artery. This one is
CC connected to either the left or the right ventricle.
CC {ECO:0000269|PubMed:10574770}.
CC -!- SIMILARITY: Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.
CC {ECO:0000305}.
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DR EMBL; U57720; AAC53042.1; -; mRNA.
DR EMBL; BC100705; AAI00706.1; -; mRNA.
DR EMBL; BC100706; AAI00707.1; -; mRNA.
DR EMBL; BC100708; AAI00709.1; -; mRNA.
DR EMBL; BC100711; AAI00712.1; -; mRNA.
DR EMBL; AF242430; AAF76323.1; -; Genomic_DNA.
DR CCDS; CCDS14869.1; -.
DR RefSeq; NP_031711.1; NM_007685.2.
DR AlphaFoldDB; P97766; -.
DR BioGRID; 198681; 3.
DR STRING; 10090.ENSMUSP00000027298; -.
DR GlyGen; P97766; 1 site.
DR PhosphoSitePlus; P97766; -.
DR PaxDb; P97766; -.
DR PRIDE; P97766; -.
DR DNASU; 12627; -.
DR Ensembl; ENSMUST00000027298; ENSMUSP00000027298; ENSMUSG00000026124.
DR GeneID; 12627; -.
DR KEGG; mmu:12627; -.
DR UCSC; uc007aow.1; mouse.
DR CTD; 55997; -.
DR MGI; MGI:109448; Cfc1.
DR VEuPathDB; HostDB:ENSMUSG00000026124; -.
DR eggNOG; KOG1217; Eukaryota.
DR GeneTree; ENSGT00940000162302; -.
DR HOGENOM; CLU_092661_0_0_1; -.
DR InParanoid; P97766; -.
DR OMA; SHCDLKS; -.
DR OrthoDB; 1387592at2759; -.
DR PhylomeDB; P97766; -.
DR TreeFam; TF333187; -.
DR BioGRID-ORCS; 12627; 2 hits in 70 CRISPR screens.
DR PRO; PR:P97766; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P97766; protein.
DR Bgee; ENSMUSG00000026124; Expressed in notochordal plate and 24 other tissues.
DR Genevisible; P97766; MM.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0070697; F:activin receptor binding; IPI:UniProtKB.
DR GO; GO:0038100; F:nodal binding; ISO:MGI.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0048856; P:anatomical structure development; IGI:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IGI:MGI.
DR GO; GO:0060413; P:atrial septum morphogenesis; IMP:MGI.
DR GO; GO:0001568; P:blood vessel development; IBA:GO_Central.
DR GO; GO:0060976; P:coronary vasculature development; IMP:MGI.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0048546; P:digestive tract morphogenesis; IMP:MGI.
DR GO; GO:0007492; P:endoderm development; IGI:MGI.
DR GO; GO:0007369; P:gastrulation; IEA:UniProtKB-KW.
DR GO; GO:0007507; P:heart development; IBA:GO_Central.
DR GO; GO:0001947; P:heart looping; IMP:MGI.
DR GO; GO:0003007; P:heart morphogenesis; IMP:MGI.
DR GO; GO:0060460; P:left lung morphogenesis; IMP:MGI.
DR GO; GO:0001889; P:liver development; IMP:MGI.
DR GO; GO:0038092; P:nodal signaling pathway; IMP:UniProtKB.
DR GO; GO:0009791; P:post-embryonic development; IMP:MGI.
DR GO; GO:0060541; P:respiratory system development; IMP:MGI.
DR GO; GO:0048536; P:spleen development; IMP:MGI.
DR InterPro; IPR019011; Cryptic/Cripto_CFC-dom.
DR InterPro; IPR000742; EGF-like_dom.
DR Pfam; PF09443; CFC; 1.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS50026; EGF_3; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Developmental protein; Disulfide bond; EGF-like domain;
KW Gastrulation; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..166
FT /note="Cryptic protein"
FT /id="PRO_0000044631"
FT PROPEP 167..202
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000395409"
FT DOMAIN 94..123
FT /note="EGF-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT LIPID 166
FT /note="GPI-anchor amidated aspartate"
FT /evidence="ECO:0000250"
FT CARBOHYD 65
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 98..105
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 99..111
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT DISULFID 113..122
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT CONFLICT 83
FT /note="P -> T (in Ref. 2; AAI00707)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 202 AA; 21792 MW; 57035AD339A16FD7 CRC64;
MRANSPTQGI SLKMHQARPL FLVTVALQLI GLGYSYQSEG DGAREVSNIL SPVIPGTTLD
RTLSNSSRKN DIPEGARLWD SLPDSSTLGE SAVPVSRCCH NGGTCVLGSF CVCPAYFTGR
YCEHDQRRRD CGALGHGAWT LHSCRLCRCI FSALYCLPHQ TFSHCDLKSF LSSGARGSRE
CSIPSLLLLV LCLLLQGVAG KG
