ID   CFA45_BOVIN             Reviewed;         549 AA.
AC   Q32LN4;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 2.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Cilia- and flagella-associated protein 45;
GN   Name=CFAP45 {ECO:0000312|VGNC:VGNC:27244};
GN   Synonyms=CCDC19 {ECO:0000312|EMBL:AAI09499.2};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913 {ECO:0000312|EMBL:AAI09499.2};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Hereford;
RX   PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA   Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA   Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA   Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT   "A whole-genome assembly of the domestic cow, Bos taurus.";
RL   Genome Biol. 10:R42.01-R42.10(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0007744|PDB:7RRO}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), FUNCTION, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA   Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA   Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA   Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT   "De novo identification of mammalian ciliary motility proteins using cryo-
RT   EM.";
RL   Cell 184:5791-5806.e19(2021).
CC   -!- FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated
CC       doublet microtubules (DMTs) in cilia axoneme, which is required for
CC       motile cilia beating (PubMed:34715025). It is an AMP-binding protein
CC       that may facilitate dynein ATPase-dependent ciliary and flagellar
CC       beating via adenine nucleotide homeostasis. May function as a donor of
CC       AMP to AK8 and hence promote ADP production (By similarity).
CC       {ECO:0000250|UniProtKB:Q9UL16, ECO:0000269|PubMed:34715025}.
CC   -!- SUBUNIT: Interacts with AK8; dimerization with AK8 may create a cavity
CC       at the interface of the dimer that can accommodate AMP. Interacts with
CC       CFAP52. Interacts with ENKUR. Directly interacts with DNALI1. Interacts
CC       with DNAH11. Interacts with DNAI1. {ECO:0000250|UniProtKB:Q9UL16}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC       {ECO:0000269|PubMed:34715025}. Cytoplasm, cytoskeleton, flagellum
CC       axoneme {ECO:0000250|UniProtKB:Q9UL16}. Note=Located in the proximal
CC       region of respiratory cilia. {ECO:0000250|UniProtKB:Q9UL16}.
CC   -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC       {ECO:0000269|PubMed:34715025}.
CC   -!- SIMILARITY: Belongs to the CFAP45 family. {ECO:0000305}.
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DR   EMBL; BC109498; AAI09499.2; -; mRNA.
DR   RefSeq; NP_001033308.2; NM_001038219.2.
DR   PDB; 7RRO; EM; 3.40 A; a/b/c/d=1-549.
DR   PDBsum; 7RRO; -.
DR   STRING; 9913.ENSBTAP00000024603; -.
DR   PRIDE; Q32LN4; -.
DR   Ensembl; ENSBTAT00000079630.1; ENSBTAP00000072198.1; ENSBTAG00000018487.4.
DR   GeneID; 617350; -.
DR   KEGG; bta:617350; -.
DR   CTD; 25790; -.
DR   VEuPathDB; HostDB:ENSBTAG00000018487; -.
DR   VGNC; VGNC:27244; CFAP45.
DR   eggNOG; ENOG502QPRZ; Eukaryota.
DR   GeneTree; ENSGT00730000111174; -.
DR   HOGENOM; CLU_026959_1_1_1; -.
DR   OrthoDB; 1428717at2759; -.
DR   TreeFam; TF327685; -.
DR   Proteomes; UP000009136; Chromosome 3.
DR   Bgee; ENSBTAG00000018487; Expressed in oviduct epithelium and 60 other tissues.
DR   ExpressionAtlas; Q32LN4; baseline and differential.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   InterPro; IPR033253; CFAP45.
DR   InterPro; IPR043597; TPH_dom.
DR   PANTHER; PTHR15504; PTHR15504; 1.
DR   Pfam; PF13868; TPH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW   Cytoskeleton; Flagellum; Reference proteome.
FT   CHAIN           1..549
FT                   /note="Cilia- and flagella-associated protein 45"
FT                   /id="PRO_0000456163"
FT   COILED          119..232
FT                   /evidence="ECO:0000255"
FT   COILED          259..393
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   549 AA;  65666 MW;  0B6F4E08088D85CD CRC64;
     MPLSTAGVLS SASTASNRSR NRPRYRTKAL SSEVDESLFG AIKPLTRSDS PIVLLRDKHA
     IRKTLTALGL DHKPETIQLI TRDMVRELII PTKDPSGQSL IMSPEEFERI KWASHVLTKE
     ELEAREQAFK KEKEAIVDTV TTRKKIMKQK EMVWRNNRKL SDLEEVAKER AQNLLQRANQ
     LRMEQEEELK DMKKIILNAK CHAIRDAQIL EKQLIQKELD AEEKRLDQMM EVERQKSVQR
     QEELDRKRRE ERIRGRRHIV EQMEKNQEER SLLAEQREQE KEQMLEYMEK LQEEDLRDLE
     QRHQQKLKMQ AEIKRINDEN QRQKAELLAQ EKLADQMVME FTKKKMAREA EFEAEQERIR
     REKEKEIARL RAMQEKAQDY QAEQDALRAK RNQEVADREW RRKEKENAQK KMETEAKLRK
     SRLEQVAFKE HTLAVQVQRD RDEFERILRA QREQIEKERL EEEKKATGRL QHANELRRQV
     RENQQKQVQA RIATFDEGRR LKEEAQKRRE RIEDIKRKKL EELRATGLPE KYCIEAERKA
     NIPVNTSVN