CENPP_HUMAN
ID CENPP_HUMAN Reviewed; 288 AA.
AC Q6IPU0; B3KRA5; B3KS17; Q5T9F8; Q5T9F9;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Centromere protein P;
DE Short=CENP-P;
GN Name=CENPP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Prostate, and Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH
RP CENPH; CENPI; CENPK; CENPN; CENPO; CENPQ; CENPR AND CENPU, FUNCTION, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16622420; DOI=10.1038/ncb1396;
RA Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III,
RA Desai A., Fukagawa T.;
RT "The CENP-H-I complex is required for the efficient incorporation of newly
RT synthesized CENP-A into centromeres.";
RL Nat. Cell Biol. 8:446-457(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE CENPA-CAD
RP COMPLEX WITH CENPI; CENPK; CENPL; CENPO; CENPQ; CENPR AND CENPS.
RX PubMed=16622419; DOI=10.1038/ncb1397;
RA Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III,
RA Cleveland D.W.;
RT "The human CENP-A centromeric nucleosome-associated complex.";
RL Nat. Cell Biol. 8:458-469(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a
CC complex recruited to centromeres which is involved in assembly of
CC kinetochore proteins, mitotic progression and chromosome segregation.
CC May be involved in incorporation of newly synthesized CENPA into
CC centromeres via its interaction with the CENPA-NAC complex.
CC {ECO:0000269|PubMed:16622420}.
CC -!- SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK,
CC CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex
CC interacts with the CENPA-NAC complex, at least composed of CENPA,
CC CENPC, CENPH, CENPM, CENPN, CENPT and CENPU.
CC {ECO:0000269|PubMed:16622419, ECO:0000269|PubMed:16622420}.
CC -!- INTERACTION:
CC Q6IPU0; Q8N8Y2: ATP6V0D2; NbExp=3; IntAct=EBI-10250303, EBI-3923949;
CC Q6IPU0; Q9BYV9: BACH2; NbExp=3; IntAct=EBI-10250303, EBI-1642333;
CC Q6IPU0; Q9BSF0: C2orf88; NbExp=3; IntAct=EBI-10250303, EBI-744298;
CC Q6IPU0; Q15834: CCDC85B; NbExp=3; IntAct=EBI-10250303, EBI-739674;
CC Q6IPU0; Q9BU64: CENPO; NbExp=11; IntAct=EBI-10250303, EBI-745954;
CC Q6IPU0; Q9C0F1: CEP44; NbExp=5; IntAct=EBI-10250303, EBI-744115;
CC Q6IPU0; Q08379: GOLGA2; NbExp=3; IntAct=EBI-10250303, EBI-618309;
CC Q6IPU0; Q96CS2: HAUS1; NbExp=5; IntAct=EBI-10250303, EBI-2514791;
CC Q6IPU0; Q86T90: KIAA1328; NbExp=3; IntAct=EBI-10250303, EBI-3437878;
CC Q6IPU0; Q6P597: KLC3; NbExp=3; IntAct=EBI-10250303, EBI-1643885;
CC Q6IPU0; O95983-2: MBD3; NbExp=3; IntAct=EBI-10250303, EBI-11978579;
CC Q6IPU0; Q02575: NHLH1; NbExp=3; IntAct=EBI-10250303, EBI-3930567;
CC Q6IPU0; Q5T203: NHLH1; NbExp=3; IntAct=EBI-10250303, EBI-10197511;
CC Q6IPU0; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-10250303, EBI-741158;
CC Q6IPU0; Q96KN3: PKNOX2; NbExp=3; IntAct=EBI-10250303, EBI-2692890;
CC Q6IPU0; Q9Y2Y1: POLR3K; NbExp=3; IntAct=EBI-10250303, EBI-11023785;
CC Q6IPU0; P49247: RPIA; NbExp=8; IntAct=EBI-10250303, EBI-744831;
CC Q6IPU0; Q9UJW9: SERTAD3; NbExp=3; IntAct=EBI-10250303, EBI-748621;
CC Q6IPU0; P61764: STXBP1; NbExp=3; IntAct=EBI-10250303, EBI-960169;
CC Q6IPU0; Q8IYF3: TEX11; NbExp=3; IntAct=EBI-10250303, EBI-742397;
CC Q6IPU0; Q96NM4-3: TOX2; NbExp=3; IntAct=EBI-10250303, EBI-12815137;
CC Q6IPU0; Q8N6Y0: USHBP1; NbExp=6; IntAct=EBI-10250303, EBI-739895;
CC Q6IPU0; Q9BYN7: ZNF341; NbExp=3; IntAct=EBI-10250303, EBI-9089622;
CC Q6IPU0; Q7L2R6-2: ZNF765; NbExp=3; IntAct=EBI-10250303, EBI-12834294;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16622420}.
CC Chromosome, centromere {ECO:0000269|PubMed:16622420}. Note=Localizes
CC exclusively in the centromeres. The CENPA-CAD complex is probably
CC recruited on centromeres by the CENPA-NAC complex.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6IPU0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6IPU0-2; Sequence=VSP_055368, VSP_055369;
CC -!- SIMILARITY: Belongs to the CENP-P/CTF19 family. {ECO:0000305}.
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DR EMBL; AK091247; BAG52317.1; -; mRNA.
DR EMBL; AK092603; BAG52579.1; -; mRNA.
DR EMBL; AL136097; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL137848; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL157827; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC071726; AAH71726.1; -; mRNA.
DR CCDS; CCDS35063.1; -. [Q6IPU0-1]
DR CCDS; CCDS69618.1; -. [Q6IPU0-2]
DR RefSeq; NP_001012267.1; NM_001012267.2. [Q6IPU0-1]
DR RefSeq; NP_001273900.1; NM_001286971.1. [Q6IPU0-2]
DR AlphaFoldDB; Q6IPU0; -.
DR BioGRID; 135138; 48.
DR ComplexPortal; CPX-5646; Kinetochore CCAN complex.
DR CORUM; Q6IPU0; -.
DR IntAct; Q6IPU0; 33.
DR MINT; Q6IPU0; -.
DR STRING; 9606.ENSP00000364737; -.
DR iPTMnet; Q6IPU0; -.
DR MetOSite; Q6IPU0; -.
DR PhosphoSitePlus; Q6IPU0; -.
DR BioMuta; CENPP; -.
DR DMDM; 74748817; -.
DR EPD; Q6IPU0; -.
DR jPOST; Q6IPU0; -.
DR MassIVE; Q6IPU0; -.
DR MaxQB; Q6IPU0; -.
DR PaxDb; Q6IPU0; -.
DR PeptideAtlas; Q6IPU0; -.
DR PRIDE; Q6IPU0; -.
DR ProteomicsDB; 3591; -.
DR ProteomicsDB; 66462; -. [Q6IPU0-1]
DR Antibodypedia; 13721; 141 antibodies from 28 providers.
DR DNASU; 401541; -.
DR Ensembl; ENST00000375579.7; ENSP00000364729.3; ENSG00000188312.14. [Q6IPU0-2]
DR Ensembl; ENST00000375587.8; ENSP00000364737.3; ENSG00000188312.14. [Q6IPU0-1]
DR GeneID; 401541; -.
DR KEGG; hsa:401541; -.
DR MANE-Select; ENST00000375587.8; ENSP00000364737.3; NM_001012267.3; NP_001012267.1.
DR UCSC; uc004arz.5; human. [Q6IPU0-1]
DR CTD; 401541; -.
DR DisGeNET; 401541; -.
DR GeneCards; CENPP; -.
DR HGNC; HGNC:32933; CENPP.
DR HPA; ENSG00000188312; Tissue enhanced (skin).
DR MIM; 611505; gene.
DR neXtProt; NX_Q6IPU0; -.
DR OpenTargets; ENSG00000188312; -.
DR PharmGKB; PA145149143; -.
DR VEuPathDB; HostDB:ENSG00000188312; -.
DR eggNOG; ENOG502S17P; Eukaryota.
DR GeneTree; ENSGT00390000011897; -.
DR HOGENOM; CLU_084497_0_0_1; -.
DR InParanoid; Q6IPU0; -.
DR OMA; TYAEWYE; -.
DR OrthoDB; 1095804at2759; -.
DR PhylomeDB; Q6IPU0; -.
DR TreeFam; TF333784; -.
DR PathwayCommons; Q6IPU0; -.
DR Reactome; R-HSA-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR Reactome; R-HSA-2467813; Separation of Sister Chromatids.
DR Reactome; R-HSA-2500257; Resolution of Sister Chromatid Cohesion.
DR Reactome; R-HSA-5663220; RHO GTPases Activate Formins.
DR Reactome; R-HSA-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR Reactome; R-HSA-68877; Mitotic Prometaphase.
DR Reactome; R-HSA-9648025; EML4 and NUDC in mitotic spindle formation.
DR SignaLink; Q6IPU0; -.
DR SIGNOR; Q6IPU0; -.
DR BioGRID-ORCS; 401541; 209 hits in 1084 CRISPR screens.
DR ChiTaRS; CENPP; human.
DR GenomeRNAi; 401541; -.
DR Pharos; Q6IPU0; Tbio.
DR PRO; PR:Q6IPU0; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q6IPU0; protein.
DR Bgee; ENSG00000188312; Expressed in calcaneal tendon and 97 other tissues.
DR ExpressionAtlas; Q6IPU0; baseline and differential.
DR Genevisible; Q6IPU0; HS.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0034080; P:CENP-A containing chromatin assembly; IEA:InterPro.
DR InterPro; IPR027801; CENP-P.
DR PANTHER; PTHR28577; PTHR28577; 1.
DR Pfam; PF13096; CENP-P; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Centromere; Chromosome; Coiled coil; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..288
FT /note="Centromere protein P"
FT /id="PRO_0000249506"
FT COILED 1..71
FT /evidence="ECO:0000255"
FT MOD_RES 38
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..15
FT /note="MDAELAEVRALQAEI -> MEAPSWSVLQRSGDE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055368"
FT VAR_SEQ 16..188
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055369"
SQ SEQUENCE 288 AA; 33165 MW; 19D32EF404E9EA7D CRC64;
MDAELAEVRA LQAEIAALRR ACEDPPAPWE EKSRVQKSFQ AIHQFNLEGW KSSKDLKNQL
GHLESELSFL STLTGINIRN HSKQTEDLTS TEMTEKSIRK VLQRHRLSGN CHMVTFQLEF
QILEIQNKER LSSAVTDLNI IMEPTECSEL SEFVSRAEER KDLFMFFRSL HFFVEWFEYR
KRTFKHLKEK YPDAVYLSEG PSSCSMGIRS ASRPGFELVI VWRIQIDEDG KVFPKLDLLT
KVPQRALELD KNRAIETAPL SFRTLVGLLG IEAALESLIK SLCAEENN
