ID   CENPN_RAT               Reviewed;         340 AA.
AC   Q5U2W4;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Centromere protein N;
DE            Short=CENP-N;
GN   Name=Cenpn;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a
CC       complex that plays a central role in assembly of kinetochore proteins,
CC       mitotic progression and chromosome segregation. The CENPA-NAC complex
CC       recruits the CENPA-CAD (nucleosome distal) complex and may be involved
CC       in incorporation of newly synthesized CENPA into centromeres. CENPN is
CC       the first protein to bind specifically to CENPA nucleosomes and the
CC       direct binding of CENPA nucleosomes by CENPN is required for centromere
CC       assembly. Required for chromosome congression and efficiently align the
CC       chromosomes on a metaphase plate. {ECO:0000250|UniProtKB:Q96H22}.
CC   -!- SUBUNIT: Component of the CENPA-NAC complex, at least composed of
CC       CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC
CC       complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK,
CC       CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with
CC       CENPA. Identified in a centromere complex containing histones H2A, H2B
CC       and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H,
CC       SSRP1 and RSF1. {ECO:0000250|UniProtKB:Q96H22}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC       {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC       Note=Localizes exclusively in the kinetochore domain of centromeres.
CC       Kinetochore-bound levels decrease when cells enter mitosis and increase
CC       again when cells exit mitosis. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CENP-N/CHL4 family. {ECO:0000305}.
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DR   EMBL; BC085839; AAH85839.1; -; mRNA.
DR   RefSeq; NP_001008367.1; NM_001008366.1.
DR   RefSeq; XP_006255748.1; XM_006255686.3.
DR   RefSeq; XP_006255749.1; XM_006255687.3.
DR   RefSeq; XP_006255751.1; XM_006255689.3.
DR   RefSeq; XP_006255752.1; XM_006255690.3.
DR   AlphaFoldDB; Q5U2W4; -.
DR   SMR; Q5U2W4; -.
DR   Ensembl; ENSRNOT00000015158; ENSRNOP00000015158; ENSRNOG00000011296.
DR   GeneID; 361416; -.
DR   KEGG; rno:361416; -.
DR   UCSC; RGD:1310953; rat.
DR   CTD; 55839; -.
DR   RGD; 1310953; Cenpn.
DR   GeneTree; ENSGT00390000004738; -.
DR   InParanoid; Q5U2W4; -.
DR   OrthoDB; 1145159at2759; -.
DR   Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR   Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-RNO-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR   Reactome; R-RNO-68877; Mitotic Prometaphase.
DR   Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q5U2W4; -.
DR   Proteomes; UP000002494; Chromosome 19.
DR   Bgee; ENSRNOG00000011296; Expressed in testis and 19 other tissues.
DR   ExpressionAtlas; Q5U2W4; baseline and differential.
DR   Genevisible; Q5U2W4; RN.
DR   GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0007059; P:chromosome segregation; IEA:InterPro.
DR   GO; GO:0051382; P:kinetochore assembly; IEA:InterPro.
DR   InterPro; IPR007902; Chl4/mis15/CENP-N.
DR   Pfam; PF05238; CENP-N; 1.
PE   2: Evidence at transcript level;
KW   Centromere; Chromosome; Kinetochore; Nucleus; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..340
FT                   /note="Centromere protein N"
FT                   /id="PRO_0000249496"
FT   MOD_RES         227
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96H22"
FT   MOD_RES         236
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96H22"
SQ   SEQUENCE   340 AA;  39459 MW;  067EFCC2929E4CAB CRC64;
     MDENVAEFLR RIILKIPLCE MKTILEAWDF LSEDQLQSIN LKQRKEFLAQ EVILLCEDKC
     ASLDDMALLD LMYTQFHRHQ KLWNVFQMSK EPGGNDVDLF DMEQFQSSFK RILQRALKNV
     TVSFRVYEKN SVWIRVAWGT QYSQPNQYKP TFVVYYPQTP YVFISSCYLK STVPLLHQAL
     KVASKHHQMA QLDLRSRHLD SLTAIVFNEY DQTFENYNST ASWREGSLGL KTDLDSKIIH
     ENTEEKVRIH RATQEAFGAY PQPQLEFAQY KLETKFKSDV GGGILADRKE PLRCLVKFSS
     PHLLEALKSL APAGIADTPL SPLLTCIPSK KMNYFKIRDK