ID   CCL12_HUMLU             Reviewed;         723 AA.
AC   M4IRL9;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=Probable CoA ligase CCL12 {ECO:0000305|PubMed:23300257};
DE            Short=HlCCL12 {ECO:0000303|PubMed:23300257};
DE            EC=6.2.1.- {ECO:0000305|PubMed:23300257};
GN   Name=CCL12 {ECO:0000303|PubMed:23300257};
OS   Humulus lupulus (European hop).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Humulus.
OX   NCBI_TaxID=3486;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Nugget;
RX   PubMed=23300257; DOI=10.1093/mp/sst004;
RA   Xu H., Zhang F., Liu B., Huhman D.V., Sumner L.W., Dixon R.A., Wang G.;
RT   "Characterization of the formation of branched short-chain fatty acid:CoAs
RT   for bitter acid biosynthesis in hop glandular trichomes.";
RL   Mol. Plant 6:1301-1317(2013).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:M4IRL4}.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are involved in
CC       the substrate recognition, and are sufficient to confer the substrate
CC       specificity. {ECO:0000250|UniProtKB:Q42524}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; JQ740214; AGA17929.1; -; mRNA.
DR   AlphaFoldDB; M4IRL9; -.
DR   SMR; M4IRL9; -.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016405; F:CoA-ligase activity; ISS:UniProtKB.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN           1..723
FT                   /note="Probable CoA ligase CCL12"
FT                   /id="PRO_0000452957"
FT   REGION          429..498
FT                   /note="SBD1"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   REGION          499..558
FT                   /note="SBD2"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   BINDING         360..368
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         498..503
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         591
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         603..606
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         703
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
SQ   SEQUENCE   723 AA;  79958 MW;  280FA28E9A207CE2 CRC64;
     MCEVGVEDLV KAGLSTEEAK YFQRVLEDIV NGAKGLDPSE VWREVVARRL LKPSHPHELH
     QLLYHSVYAN WDVSTRGPPL YWFPSLHQSK KTNLGRLMEK YGSEVLGPLY KDPITSFSLF
     RKFSVQQPEA YWSIVLKELS VSFQEAPKCI LDSADKSKRS GSLLPGSVLN IAECCLLPTS
     YPMKRDDSPA IVWRDEGYDD SEVNVLTLKE LREKVMLVAN ALDAMFSKGD AIAIDMPMTV
     NAVVIYLAII LSGFVVVSIA DSFAAKEIAT RLRVSNAKAI FTQDFILRGG RKFSLYSRVV
     DAAPHKAIVL PVNGSNLGLQ LREQDISWEK FISNVSNQAR SNHYTPFYQP VDSLINILFS
     SGTTGEPKAI PWTQLSPLRC AADTWAHIDA QVGDVFCWPT NLGWVMGPVL LFSSFLSGAT
     LALYHGSPLG HGFGKFVQDS GVTILGTVPS LVKAWKNTQC MKGLDWTKIK VFASTGEASN
     VDDDLWLSSQ AYYQPIIECC GGTELASSYI QGSLVQPQAF GAFSTAAMTV GLVILDENGI
     PYPDKQECTG EVGLFPLYLG ATDRLLNADN EKVYFKGMPL YNGMSLRRHG DILKRTAGGF
     FIVHGRADDT MNLGGIKTSS IEIERVCNRA DESVMETTAV SVAPVSGGPE QLVMFVVLKS
     GHNNEAERLK KKFSKAIQSN LNPLFKVSFV KIVEEFPRTA SNKLLRRVLR DQMKHELLVR
     SKI