ID   ZYS3_CHLRE              Reviewed;         371 AA.
AC   Q8GSP8; Q94F59; Q9SXM3; Q9SXM4;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Zygote-specific protein 3;
DE   Flags: Precursor;
GN   Name=ZYS3;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAA76762.1}
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=137c / CC-125 {ECO:0000269|PubMed:10069826};
RX   PubMed=10069826; DOI=10.1104/pp.119.3.873;
RA   Kuriyama H., Takano H., Suzuki H., Uchida H., Kawano S., Kuroiwa H.,
RA   Kuroiwa T.;
RT   "Characterization of Chlamydomonas reinhardtii zygote-specific cDNAs that
RT   encode novel proteins containing ankyrin repeats and WW domains.";
RL   Plant Physiol. 119:873-884(1999).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:BAC54284.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
RC   STRAIN=137c / CC-125;
RA   Uchida H., Suzuki K., Tanifuji G., Hara Y.;
RT   "Isolation and characterization of zys3 gene in Chlamydomonas
RT   reinhardtii.";
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305, ECO:0000312|EMBL:BAC54284.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-334 (ISOFORM 1).
RA   Woessner J.P.;
RL   Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May have a role in the remodeling of the endoplasmic
CC       reticulum upon zygote formation. {ECO:0000303|PubMed:10069826}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC       {ECO:0000269|PubMed:10069826}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1 {ECO:0000269|PubMed:10069826};
CC         IsoId=Q8GSP8-1; Sequence=Displayed;
CC       Name=2 {ECO:0000269|PubMed:10069826};
CC         IsoId=Q8GSP8-2; Sequence=VSP_014165;
CC   -!- DEVELOPMENTAL STAGE: Isoform 1 and isoform 2 are expressed 2-22 hours
CC       after zygote formation with highest level at 6 hours. Isoform 2 is
CC       expressed at low level. {ECO:0000269|PubMed:10069826}.
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DR   EMBL; AB004042; BAA76762.1; -; mRNA.
DR   EMBL; AB004043; BAA76863.1; -; mRNA.
DR   EMBL; AB091098; BAC54284.1; -; Genomic_DNA.
DR   EMBL; AF385612; AAK67153.1; -; Genomic_DNA.
DR   RefSeq; XP_001700598.1; XM_001700546.1. [Q8GSP8-1]
DR   AlphaFoldDB; Q8GSP8; -.
DR   SMR; Q8GSP8; -.
DR   STRING; 3055.EDO97927; -.
DR   EnsemblPlants; PNW72600; PNW72600; CHLRE_15g635400v5. [Q8GSP8-1]
DR   GeneID; 5726149; -.
DR   Gramene; PNW72600; PNW72600; CHLRE_15g635400v5. [Q8GSP8-1]
DR   KEGG; cre:CHLRE_15g635400v5; -.
DR   HOGENOM; CLU_886696_0_0_1; -.
DR   OMA; TVQGHPI; -.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IDA:UniProtKB.
DR   GO; GO:0048468; P:cell development; NAS:UniProtKB.
DR   CDD; cd00201; WW; 1.
DR   Gene3D; 1.25.40.20; -; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR001202; WW_dom.
DR   InterPro; IPR036020; WW_dom_sf.
DR   Pfam; PF12796; Ank_2; 1.
DR   Pfam; PF00397; WW; 1.
DR   SMART; SM00248; ANK; 2.
DR   SMART; SM00456; WW; 2.
DR   SUPFAM; SSF48403; SSF48403; 1.
DR   SUPFAM; SSF51045; SSF51045; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 1.
DR   PROSITE; PS01159; WW_DOMAIN_1; 2.
DR   PROSITE; PS50020; WW_DOMAIN_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ANK repeat; Endoplasmic reticulum; Glycoprotein;
KW   Repeat; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..371
FT                   /note="Zygote-specific protein 3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000001623"
FT   REPEAT          62..91
FT                   /note="ANK 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          94..123
FT                   /note="ANK 2"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          159..187
FT                   /note="WW 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT   DOMAIN          283..313
FT                   /note="WW 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00224"
FT   CARBOHYD        41
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         79..104
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10069826"
FT                   /id="VSP_014165"
FT   CONFLICT        261
FT                   /note="A -> S (in Ref. 1; BAA76762/BAA76863)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   371 AA;  39336 MW;  DCC0011986611035 CRC64;
     MLRSAGRVAA VALLALFALG CVSAAAKPST DRLVAAIKSK NLSAVVDALS VKGLDVNTPD
     STRRLPLVEA ARSRDARLVG ALLDQGALAR VSDGTTTPLH LSMQGGSAAI VKLLLAHGAD
     PNAKDKTGAS ARSTAAAVKE LADLLKQWDA RGAMAFEDEP GAWIREERDG QSYYWKPANG
     ESRWAVPPSC AWQRVTVQGH PIKYINSLTG QETTRVPPAL AWARVTAADG SALWLNWASR
     VASAAATAPA ELPAELAAEL AMHPNRRWYN TATREYVYTD PAYATPWREL VDEASGAPFF
     FNVETGDTTW ELPAALAWTE VIESSSGADG ESGSGSEAGP RYFHNTVSGE VAWSAPEGSR
     HVFVEASAAD L