ZN148_DANRE
ID ZN148_DANRE Reviewed; 808 AA.
AC A0MS83; B0S5L9; B3DIY1;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Zinc finger protein 148;
DE AltName: Full=Transcription factor ZBP-89;
DE AltName: Full=Zinc finger DNA-binding protein 89;
GN Name=znf148; Synonyms=zbp89; ORFNames=si:dkey-101k6.6;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=16914492; DOI=10.1242/dev.02540;
RA Li X., Xiong J.-W., Shelley C.S., Park H., Arnaout M.A.;
RT "The transcription factor ZBP-89 controls generation of the hematopoietic
RT lineage in zebrafish and mouse embryonic stem cells.";
RL Development 133:3641-3650(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in transcriptional regulation. Represses the
CC transcription of a number of genes. Required for primitive and
CC definitive hematopoiesis during embryonic development.
CC {ECO:0000269|PubMed:16914492}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Detected in posterior lateral mesoderm, head
CC mesenchyme and the intermediate cell mass in embryos 24 hours after
CC fertilization. {ECO:0000269|PubMed:16914492}.
CC -!- MISCELLANEOUS: Knock-down of the znf148 mRNA disrupts both primitive
CC and definitive hematopoiesis, but does not affect primary blood vessel
CC formation. Most embryos die before four days after fertilization.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; EF032416; ABK40532.1; -; mRNA.
DR EMBL; BX255930; CAQ14829.1; -; Genomic_DNA.
DR EMBL; BC163289; AAI63289.1; -; mRNA.
DR RefSeq; NP_001073649.1; NM_001080180.1.
DR AlphaFoldDB; A0MS83; -.
DR SMR; A0MS83; -.
DR STRING; 7955.ENSDARP00000071856; -.
DR PaxDb; A0MS83; -.
DR Ensembl; ENSDART00000077389; ENSDARP00000071856; ENSDARG00000055106.
DR Ensembl; ENSDART00000184890; ENSDARP00000155940; ENSDARG00000055106.
DR GeneID; 560300; -.
DR KEGG; dre:560300; -.
DR CTD; 7707; -.
DR ZFIN; ZDB-GENE-030131-8769; znf148.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000157406; -.
DR HOGENOM; CLU_025987_1_0_1; -.
DR InParanoid; A0MS83; -.
DR OMA; FEQNFKS; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; A0MS83; -.
DR TreeFam; TF331779; -.
DR Reactome; R-DRE-212436; Generic Transcription Pathway.
DR PRO; PR:A0MS83; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 9.
DR Bgee; ENSDARG00000055106; Expressed in caudal fin and 24 other tissues.
DR ExpressionAtlas; A0MS83; baseline.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030097; P:hemopoiesis; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 4.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..808
FT /note="Zinc finger protein 148"
FT /id="PRO_0000354690"
FT ZN_FING 180..202
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 208..230
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 236..258
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 264..287
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 15..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 131..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 305..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 596..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 705..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..74
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 66
FT /note="E -> DD (in Ref. 3; AAI63289)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="K -> T (in Ref. 1; ABK40532)"
FT /evidence="ECO:0000305"
FT CONFLICT 429
FT /note="Q -> E (in Ref. 3; AAI63289)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 808 AA; 90507 MW; B87251E494294A77 CRC64;
MNIDDKLEGM LLKYSPVGLQ HPSRGLGPSR VDGRGRRGSL DMTLGERSLA NHPLLAEDDD
DEDEEEDDDD DLAGEGLTSH DLISQDDLMV HEETVKNDGQ DEAAFSHRIS NKLHYTLSMP
VNIKQEMKVP DSLILNKKEK KPGRDPSDCH KKKKRKQRSP AKILTINEDG SLGHQNPKSH
ICEHCNAAFR TNYHLQRHVF IHTGEKPFQC NQCDMRFIQK YLLQRHEKIH TGEKPFRCDE
CGMKFIQKYH MERHKRTHSG EKPYQCDYCH QFFSRTDRVL KHRRMCHENK DRKVQKTAVK
DSPLRTPENL GFSFPAKDCT LPKKKRQKTS DKSRASITNP AVDKVVEADM DEKTEDRLAK
SECLPLYAVA TKVKDEYVVT DYSVELPDSP PGSRHLAGEE SNDEIISPPK LVLKKIASRR
GFKQQALEQS QSLSPLSSFE ESKVTRYTFE IVDNKGLLDV ETNPDMESVE ALQVGQAKPT
GSSTNYDDAM QFVKKRRYLQ AATANTSRDY ALNVSSIVSQ SSVTQASVAT VIDETVPATI
LSETQTLNVE IKSSNEKNVL PDEVLQTLLD HYSNKANGQA EISFSVADTE VTSSISINSS
DESSPAEALV TSSQAPPTEK ASLLQEYSKF LQQALERTSQ NDTYLNNQSL TFVNDSASLA
GQPLFSTEKQ FTSPPRFRPT MNSPLRSTLE KPNFNLLVDT QHSFSFSGDE TNPSSVSPTE
DFLDQVTSPK KTDPQSISQT FQITTFDQNF RSQFPSSRSG ISPQFSIASG QVTLRGHGGA
DFPEFSLVNE TRTQLTSSPD ATSSQTFG
