ID   Y3134_MYCTO             Reviewed;         268 AA.
AC   P9WFD2; L0TEM6; P95192; Q7D624;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Universal stress protein MT3220;
GN   OrderedLocusNames=MT3220;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, DORMANCY REGULON, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
CC   -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC       reduced oxygen tension (hypoxia) and low levels of nitric oxide (NO).
CC       {ECO:0000269|PubMed:12953092}.
CC   -!- DISRUPTION PHENOTYPE: A strain lacking this gene does not induce most
CC       genes of the dormancy regulon, due to polar effects on the downstream
CC       devR (dosR) gene. Restoration of induction only occurs when both this
CC       gene and devR (dosR) are transformed into the deleted strain.
CC       {ECO:0000269|PubMed:12953092}.
CC   -!- SIMILARITY: Belongs to the universal stress protein A family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK47558.1; -; Genomic_DNA.
DR   PIR; G70645; G70645.
DR   RefSeq; WP_003416373.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WFD2; -.
DR   SMR; P9WFD2; -.
DR   EnsemblBacteria; AAK47558; AAK47558; MT3220.
DR   GeneID; 45427120; -.
DR   KEGG; mtc:MT3220; -.
DR   PATRIC; fig|83331.31.peg.3470; -.
DR   HOGENOM; CLU_049301_2_3_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.620; -; 2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR006015; Universal_stress_UspA.
DR   InterPro; IPR006016; UspA.
DR   Pfam; PF00582; Usp; 1.
DR   PRINTS; PR01438; UNVRSLSTRESS.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding.
FT   CHAIN           1..268
FT                   /note="Universal stress protein MT3220"
FT                   /id="PRO_0000428559"
FT   BINDING         13
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P9WFD7"
FT   BINDING         107..113
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P9WFD7"
FT   BINDING         117
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P9WFD7"
FT   BINDING         120..121
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P9WFD7"
SQ   SEQUENCE   268 AA;  28008 MW;  EE2CB4F9C9D602AE CRC64;
     MSDPRPARAV VVGIDGSRAA THAALWAVDE AVNRDIPLRL VYVIDPSQLS AAGEGGGQSA
     ARAALHDASR KVEATGQPVK IETEVLCGRP LTKLMQESRS AAMLCVGSVG LDHVRGRRGS
     VAATLAGSAL CPVAVIHPSP AEPATTSQVS AVVAEVDNGV VLRHAFEEAR LRGVPLRAVA
     VHAAETPDDV EQGSRLAHVH LSRRLAHWTR LYPEVRVDRA IAGGSACRHL AANAKPGQLF
     VADSHSAHEL CGAYQPGCAV LTVRSANL