CAR4_DICDI
ID CAR4_DICDI Reviewed; 443 AA.
AC Q9TX43; Q54YI6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Cyclic AMP receptor 4;
DE Short=cAMP receptor 4;
GN Name=carD; Synonyms=car4; ORFNames=DDB_G0277831;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE, FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=NC-4;
RX PubMed=7958880; DOI=10.1101/gad.8.17.2086;
RA Louis J.M., Ginsburg G.T., Kimmel A.R.;
RT "The cAMP receptor CAR4 regulates axial patterning and cellular
RT differentiation during late development of Dictyostelium.";
RL Genes Dev. 8:2086-2096(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Receptor for cAMP. Regulates axial patterning and cellular
CC differentiation during late development. The activity of this receptor
CC is mediated by G proteins. {ECO:0000269|PubMed:7958880}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- DEVELOPMENTAL STAGE: Initially expressed during tip elongation and
CC continues to accumulate into culmination. {ECO:0000269|PubMed:7958880}.
CC -!- PTM: C-terminal Ser or Thr residues may be phosphorylated.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 5 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000023; EAL68088.1; -; Genomic_DNA.
DR PIR; A54813; A54813.
DR RefSeq; XP_642216.1; XM_637124.1.
DR AlphaFoldDB; Q9TX43; -.
DR SMR; Q9TX43; -.
DR STRING; 44689.DDB0216256; -.
DR PaxDb; Q9TX43; -.
DR EnsemblProtists; EAL68088; EAL68088; DDB_G0277831.
DR GeneID; 8621423; -.
DR KEGG; ddi:DDB_G0277831; -.
DR dictyBase; DDB_G0277831; carD.
DR HOGENOM; CLU_050319_0_0_1; -.
DR InParanoid; Q9TX43; -.
DR OMA; NDCALAI; -.
DR PhylomeDB; Q9TX43; -.
DR PRO; PR:Q9TX43; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030552; F:cAMP binding; IDA:dictyBase.
DR GO; GO:0001646; F:cAMP receptor activity; ISS:dictyBase.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:dictyBase.
DR GO; GO:0071901; P:negative regulation of protein serine/threonine kinase activity; IMP:dictyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:dictyBase.
DR GO; GO:1903615; P:positive regulation of protein tyrosine phosphatase activity; IMP:dictyBase.
DR GO; GO:0007165; P:signal transduction; TAS:dictyBase.
DR InterPro; IPR022343; GCR1-cAMP_receptor.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000848; GPCR_cAMP.
DR PRINTS; PR02001; GCR1CAMPR.
DR PRINTS; PR00247; GPCRCAMP.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..443
FT /note="Cyclic AMP receptor 4"
FT /id="PRO_0000195083"
FT TOPO_DOM 1..11
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..89
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..119
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..161
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..263
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 325..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..443
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..427
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..443
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 122
FT /note="F -> I (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 164..165
FT /note="LF -> YI (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 323
FT /note="H -> HH (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="N -> I (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 51467 MW; F6EDF7F32E180CB4 CRC64;
MKVLQEINLT YSILVIADFS SIFGCLLVLI AFKKLKLLRN HITRVIACFC VSSLLKDIIS
TGLTLSLGPQ NEAGSTSFQC YLYAITITYG SLACWLWTLC LAFSIYNLIV KREPEPEKYE
KFYHGVCWTI PLICVIVMLA KKTIEPVGNW CWISEKYVGY RFGLFYGPFF AIWIISAVLV
GLTSRYTYSV IRNSVSDNKD KHMTYQFKLI NYIIVFLLCW VFAIVNRILN GLGYYPTLPN
ILHTYFSVSH GFFASVTFIY NNPLMWRYWG SKIFLIFAKF GYFVELQRRL DRNKNNNNPS
PILNSYAATV YHSSTIESLS LQHNNDISND NQQQQQQQQT PQQPQQQFQQ QQSPTVIEMQ
NLKQDQNIEN NEQNENCYNT IDTNIEINTN KLNDNSFEIT QPSNDLNTIE NNNNYNNNNN
NNNNNSLVIE KEKDEREKKD NKF
