ID   Y2128_LACLA             Reviewed;         428 AA.
AC   Q9CDT3;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Putative zinc metalloprotease LL2128;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=LL2128; ORFNames=L181494;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
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DR   EMBL; AE005176; AAK06226.1; -; Genomic_DNA.
DR   PIR; H86890; H86890.
DR   RefSeq; NP_268285.1; NC_002662.1.
DR   RefSeq; WP_010906325.1; NC_002662.1.
DR   AlphaFoldDB; Q9CDT3; -.
DR   SMR; Q9CDT3; -.
DR   STRING; 272623.L181494; -.
DR   PaxDb; Q9CDT3; -.
DR   DNASU; 1115805; -.
DR   EnsemblBacteria; AAK06226; AAK06226; L181494.
DR   KEGG; lla:L181494; -.
DR   PATRIC; fig|272623.7.peg.2287; -.
DR   eggNOG; COG0750; Bacteria.
DR   HOGENOM; CLU_025778_1_0_9; -.
DR   OMA; QYMVGFG; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF17820; PDZ_6; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Metal-binding; Metalloprotease;
KW   Protease; Reference proteome; Transmembrane; Transmembrane helix; Zinc.
FT   CHAIN           1..428
FT                   /note="Putative zinc metalloprotease LL2128"
FT                   /id="PRO_0000088445"
FT   TRANSMEM        188..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        401..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          188..282
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   ACT_SITE        20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         19
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         23
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ   SEQUENCE   428 AA;  46423 MW;  5BE5447331DE6AC8 CRC64;
     MIETLITFII IFGIIVAIHE YGHLWWAKRS GILVREYAVG MGPKIFAHQA KDGTLYTIRI
     LPLGGYVRLA GWGDDKTEIK KGQAASLVVS KSEVVNPEAE NSVSNIVRRI NLSEHVELEE
     AIPMLITEYD FEKELFIEGE VFGEIKRYSV DHDATIIEED GTEVRIAPLD VQYQSAGVFH
     KMLTNFGGPL NNFILGIIAF IVLTFVQGGV PSTTNAIGQV EKGTPAYNAG LKAGDKIEAV
     NGTKTADWNN VVTEISGSKG KELKLEVSRS GKSETLSVTP KKMDGSYRVG IMQSMKTGFF
     DKITGGFVQA GQSATAIFKA LGSLIARPSL DKLGGPVAIY QLSGQAARAG FPAIVYLLAM
     LSINLGIVNL FPIPVLDGGK IVLNIIEAIR GKALSQEKES IITMVGVVFM LVLFVAVTWN
     DILRAFVN