CAR2_DICDI
ID CAR2_DICDI Reviewed; 375 AA.
AC P34907; Q54J91;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Cyclic AMP receptor 2;
DE Short=cAMP receptor 2;
GN Name=carB; Synonyms=car2; ORFNames=DDB_G0288179;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8436297; DOI=10.1101/gad.7.2.262;
RA Saxe C.L. III, Ginsburg G.T., Louis J.M., Johnson R.L., Devreotes P.N.,
RA Kimmel A.R.;
RT "CAR2, a prestalk cAMP receptor required for normal tip formation and late
RT development of Dictyostelium discoideum.";
RL Genes Dev. 7:262-272(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Receptor for cAMP. Coordinates the aggregation of individual
CC cells into a multicellular organism and regulates the expression of a
CC large number of developmentally regulated genes. The activity of this
CC receptor is mediated by G proteins. Plays a key role during tip
CC formation and late development; involved in cAMP-directed patterning of
CC pre stalk cells as they sort before and during tip formation.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- DEVELOPMENTAL STAGE: Expressed only after cells have aggregated and,
CC then, preferentially in prestalk cells.
CC -!- PTM: C-terminal Ser or Thr residues may be phosphorylated.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 5 family.
CC {ECO:0000305}.
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DR EMBL; S55234; AAB25436.2; -; Genomic_DNA.
DR EMBL; AAFI02000109; EAL63320.1; -; Genomic_DNA.
DR PIR; A46390; A46390.
DR RefSeq; XP_636845.1; XM_631753.1.
DR AlphaFoldDB; P34907; -.
DR SMR; P34907; -.
DR STRING; 44689.DDB0191393; -.
DR PaxDb; P34907; -.
DR EnsemblProtists; EAL63320; EAL63320; DDB_G0288179.
DR GeneID; 8626513; -.
DR KEGG; ddi:DDB_G0288179; -.
DR dictyBase; DDB_G0288179; carB.
DR eggNOG; ENOG502RYGP; Eukaryota.
DR HOGENOM; CLU_050319_0_0_1; -.
DR InParanoid; P34907; -.
DR OMA; GWCWIGD; -.
DR PhylomeDB; P34907; -.
DR PRO; PR:P34907; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030552; F:cAMP binding; IDA:dictyBase.
DR GO; GO:0001646; F:cAMP receptor activity; TAS:dictyBase.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IEP:dictyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IEP:dictyBase.
DR GO; GO:0007165; P:signal transduction; TAS:dictyBase.
DR InterPro; IPR022343; GCR1-cAMP_receptor.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000848; GPCR_cAMP.
DR PRINTS; PR02001; GCR1CAMPR.
DR PRINTS; PR00247; GPCRCAMP.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Membrane; Phosphoprotein; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..375
FT /note="Cyclic AMP receptor 2"
FT /id="PRO_0000195081"
FT TOPO_DOM 1..10
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..82
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..108
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..119
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..138
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..161
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..180
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..223
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..234
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..259
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 338..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..359
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..375
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 192
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MOD_RES 298
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MOD_RES 303
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT CONFLICT 92
FT /note="C -> L (in Ref. 1; AAB25436)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 43301 MW; 87E21F87B1693B41 CRC64;
MTIMSDIIAQ RTILLIADFS SIIGCSLVLI GFWRLKLLRN HITKIISLFC ATSLFKDVIS
TIITLLYKPD QTESGFPCYL HAIVITFGSL ACWLWTLMLS FSIYNLIVRR EPEPERFEKF
YFCLCYGLPL ISTIVMLSTH IIQPVGGWCW IGDNYDGYRF GLFYGPFFFI WGTSAILVGL
TSKYTYSVIR SSVSDNKDKH MTYQFKLINY IVVFLVCWVF AIVNRILNGL NQFPTVPNVL
HTYFSVSHGF YASITFIYNN PLMWRYFGAK FLLIFSKFGL FVQAQQRLEL NKNNNNPSPI
MRSKNALDNG ADSSVVELPC LSKADSLSLD AENNIETPKE NENQNHHHHH HHHHHHNHYN
NNNNNNNINN KNDMI
