ID   Y209_MYCGE              Reviewed;         308 AA.
AC   P47451;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase MG209;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=MG209;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 125-243.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; L43967; AAC71427.1; -; Genomic_DNA.
DR   EMBL; U02214; AAD12509.1; -; Genomic_DNA.
DR   PIR; A64223; A64223.
DR   RefSeq; WP_009885746.1; NZ_AAGX01000004.1.
DR   AlphaFoldDB; P47451; -.
DR   SMR; P47451; -.
DR   STRING; 243273.MG_209; -.
DR   EnsemblBacteria; AAC71427; AAC71427; MG_209.
DR   KEGG; mge:MG_209; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_4_4_14; -.
DR   OMA; AHPSPGW; -.
DR   OrthoDB; 1714699at2; -.
DR   BioCyc; MGEN243273:G1GJ2-242-MON; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..308
FT                   /note="Uncharacterized RNA pseudouridine synthase MG209"
FT                   /id="PRO_0000162739"
FT   DOMAIN          11..87
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        131
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   308 AA;  34996 MW;  F2850BAD06A125A2 CRC64;
     MKQCFVVTTT KRLDSLLASL LNLSRVKVVK LIMNGQIKVN EKLTFKNSLI VAKDDVIKVE
     IHDETTSDFI TSVEPYNLKL EVLFEDKDLM VINKPSGLLT HPTTFNEKAS LLAACIFHNN
     KNPVYLVHRL DRDTSGAIVV CKNQASLLNL QNQLQNRTLK RYYVALVHFP FNALTGSINA
     PLARVNNNKV MFKIAQTAKA KQAITKFKVI NQNEKAALIS LELLTGRTHQ IRVHLKFIQH
     PVYNDPLYGI KSEKKDSYGQ FLHANRICFI HPTLNKPMDF HAPLEPKFST KLKSLNLSLT
     DPLHVLFK