XPO1B_ARATH
ID XPO1B_ARATH Reviewed; 1076 AA.
AC F4IZR5; Q0WV73; Q94IV0; Q94KD5; Q9M9N0;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Protein EXPORTIN 1B {ECO:0000303|PubMed:18791220};
GN Name=XPO1B {ECO:0000303|PubMed:18791220};
GN OrderedLocusNames=At3g03110 {ECO:0000312|Araport:AT3G03110};
GN ORFNames=T17B22.20 {ECO:0000312|EMBL:AAF26113.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Haasen D., Merkle T.;
RT "Arabidopsis thaliana contains two genes expressing functional Exportin 1
RT proteins.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 678-1076.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 678-1076.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=18791220; DOI=10.1534/genetics.108.094896;
RA Blanvillain R., Boavida L.C., McCormick S., Ow D.W.;
RT "Exportin1 genes are essential for development and function of the
RT gametophytes in Arabidopsis thaliana.";
RL Genetics 180:1493-1500(2008).
CC -!- FUNCTION: Receptor for the leucine-rich nuclear export signal (NES).
CC Binds cooperatively to the NES on its target protein and to the small
CC GTPase Ran in its active GTP-bound form (By similarity). Required for
CC the maternal-to-embryonic transition and during gametophyte development
CC (PubMed:18791220). {ECO:0000250|UniProtKB:Q9SMV6,
CC ECO:0000269|PubMed:18791220}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:Q9TVM2}. Nucleus membrane
CC {ECO:0000250|UniProtKB:Q9TVM2}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q9TVM2}; Nucleoplasmic side
CC {ECO:0000250|UniProtKB:Q9TVM2}.
CC -!- TISSUE SPECIFICITY: Present in mature pollen grains, unpollinated
CC pistils, and 2-week-old seedlings. {ECO:0000269|PubMed:18791220}.
CC -!- DISRUPTION PHENOTYPE: Gametophyte defective when both XPO1A and XPO1B
CC are disrupted. Abnormal pollen germination and tube growth, impaired
CC female gametophyte development and embryo lethal.
CC {ECO:0000269|PubMed:18791220}.
CC -!- SIMILARITY: Belongs to the exportin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26113.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAK56267.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE98975.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ277542; CAC39223.1; -; mRNA.
DR EMBL; AC012328; AAF26113.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73903.1; -; Genomic_DNA.
DR EMBL; AF367278; AAK56267.1; ALT_INIT; mRNA.
DR EMBL; BT004508; AAO42754.1; -; mRNA.
DR EMBL; AK226898; BAE98975.1; ALT_INIT; mRNA.
DR RefSeq; NP_566193.2; NM_111181.3.
DR AlphaFoldDB; F4IZR5; -.
DR SMR; F4IZR5; -.
DR STRING; 3702.AT3G03110.1; -.
DR PaxDb; F4IZR5; -.
DR PRIDE; F4IZR5; -.
DR ProMEX; F4IZR5; -.
DR ProteomicsDB; 242526; -.
DR EnsemblPlants; AT3G03110.1; AT3G03110.1; AT3G03110.
DR GeneID; 821074; -.
DR Gramene; AT3G03110.1; AT3G03110.1; AT3G03110.
DR KEGG; ath:AT3G03110; -.
DR Araport; AT3G03110; -.
DR TAIR; locus:2097750; AT3G03110.
DR eggNOG; KOG2020; Eukaryota.
DR HOGENOM; CLU_011906_0_0_1; -.
DR InParanoid; F4IZR5; -.
DR OMA; HKICIAS; -.
DR OrthoDB; 132850at2759; -.
DR PRO; PR:F4IZR5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4IZR5; baseline and differential.
DR Genevisible; F4IZR5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005049; F:nuclear export signal receptor activity; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0009553; P:embryo sac development; IGI:TAIR.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0009846; P:pollen germination; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR GO; GO:0006611; P:protein export from nucleus; IEA:InterPro.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IBA:GO_Central.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR041123; CRM1_repeat.
DR InterPro; IPR041235; Exp1_repeat_2.
DR InterPro; IPR013598; Exportin-1/Importin-b-like.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR045065; XPO1/5.
DR InterPro; IPR014877; XPO1_C_dom.
DR InterPro; IPR040485; XPO1_repeat_3.
DR PANTHER; PTHR11223; PTHR11223; 1.
DR Pfam; PF08767; CRM1_C; 1.
DR Pfam; PF18777; CRM1_repeat; 1.
DR Pfam; PF18784; CRM1_repeat_2; 1.
DR Pfam; PF18787; CRM1_repeat_3; 1.
DR Pfam; PF03810; IBN_N; 1.
DR Pfam; PF08389; Xpo1; 1.
DR SMART; SM01102; CRM1_C; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Membrane; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Repeat; Translocation;
KW Transport.
FT CHAIN 1..1076
FT /note="Protein EXPORTIN 1B"
FT /id="PRO_0000432146"
FT DOMAIN 37..103
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REPEAT 135..171
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 232..267
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 282..319
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 475..514
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 564..601
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 613..650
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 683..720
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 757..794
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 799..836
FT /note="HEAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 895..935
FT /note="HEAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 943..988
FT /note="HEAT 11"
FT /evidence="ECO:0000255"
FT CONFLICT 940
FT /note="P -> S (in Ref. 1; CAC39223)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1076 AA; 123193 MW; 7995F65D0F1E8944 CRC64;
MAAEKLRDLS QPIDVVLLDA TVEAFYSTGS KEERASADNI LRDLKANPDT WLQVVHILQN
TSSTHTKFFA LQVLEGVIKY RWNALPVEQR DGMKNYISDV IVQLSRDEAS FRTERLYVNK
LNIILVQIVK QEWPAKWKSF IPDLVIAAKT SETICENCMA ILKLLSEEVF DFSKGEMTQQ
KIKELKQSLN SEFQLIHELC LYVLSASQRQ ELIRATLSAL HAYLSWIPLG YIFESPLLEI
LLKFFPVPAY RNLTLQCLSE VASLNFGDFY DMQYVKMYSI FMNQLQAILP LNLNIPEAYS
TGSSEEQAFI QNLALFFTSF FKLHIKILES APENISLLLA GLGYLISISY VDDTEVFKVC
LDYWNSLVLE LFGTRHHACH PALTPSLFGL QMAFLPSTVD GVKSEVTERQ KLYSDPMSKL
RGLMISRTAK PEEVLIVEDE NGNIVRETMK DNDVLVQYKI MRETLIYLSH LDHEDTEKQM
LSKLSKQLSG EEWAWNNLNT LCWAIGSISG SMVVEQENRF LVMVIRDLLS LCEVVKGKDN
KAVIASNIMY VVGQYSRFLR AHWKFLKTVV HKLFEFMHET HPGVQDMACD TFLKIVQKCK
RKFVIVQVGE SEPFVSELLS GLATIVGDLQ PHQIHTFYES VGSMIQAESD PQKRGEYLQR
LMALPNQKWA EIIGQARQSA DILKEPDVIR TVLNILQTNT RVATSLGTFF LSQISLIFLD
MLNVYRMYSE LVSSSIANGG PYASRTSLVK LLRSVKREIL KLIETFLDKA ENQPHIGKQF
VPPMMDQVLG DYARNVPDAR ESEVLSLFAT IINKYKVVMR DEVPLIFEAV FQCTLEMITK
NFEDYPEHRL KFFSLLRAIA TFCFRALIQL SSEQLKLVMD SVIWAFRHTE RNIAETGLNL
LLEMLKNFQK SDFCNKFYQT YFLQIEQEVF AVLTDTFHKP GFKLHVLVLQ HLFSLVESGS
LAEPLWDAAT VPHPYSNNVA FVLEYTTKLL SSSFPNMTTT EVTQFVNGLY ESRNDVGRFK
DNIRDFLIQS KEFSAQDNKD LYAEEAAAQM ERERQRMLSI PGLIAPSEIQ DDMADS
