WZXE_SHIFL
ID WZXE_SHIFL Reviewed; 416 AA.
AC P0AAA8; P27834;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Lipid III flippase {ECO:0000255|HAMAP-Rule:MF_02024};
GN Name=wzxE {ECO:0000255|HAMAP-Rule:MF_02024}; Synonyms=wzx;
GN OrderedLocusNames=SF3866, S3894;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Mediates the transbilayer movement of Und-PP-GlcNAc-ManNAcA-
CC Fuc4NAc (lipid III) from the inner to the outer leaflet of the
CC cytoplasmic membrane during the assembly of enterobacterial common
CC antigen (ECA). {ECO:0000255|HAMAP-Rule:MF_02024}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02024}.
CC -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC {ECO:0000255|HAMAP-Rule:MF_02024}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02024}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_02024}.
CC -!- SIMILARITY: Belongs to the polysaccharide transport (PST) (TC 2.A.66.2)
CC family. {ECO:0000255|HAMAP-Rule:MF_02024}.
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DR EMBL; AE005674; AAN45303.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP18895.1; -; Genomic_DNA.
DR RefSeq; NP_709596.1; NC_004337.2.
DR RefSeq; WP_000050265.1; NZ_WPGW01000028.1.
DR AlphaFoldDB; P0AAA8; -.
DR SMR; P0AAA8; -.
DR STRING; 198214.SF3866; -.
DR EnsemblBacteria; AAN45303; AAN45303; SF3866.
DR EnsemblBacteria; AAP18895; AAP18895; S3894.
DR GeneID; 1025971; -.
DR GeneID; 58389391; -.
DR KEGG; sfl:SF3866; -.
DR KEGG; sfx:S3894; -.
DR PATRIC; fig|198214.7.peg.4556; -.
DR HOGENOM; CLU_042154_0_0_6; -.
DR OMA; KFTIMAL; -.
DR OrthoDB; 1661399at2; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13125; MATE_like_10; 1.
DR HAMAP; MF_02024; WzxE; 1.
DR InterPro; IPR002797; Polysacc_synth.
DR InterPro; IPR044550; WzxE.
DR InterPro; IPR032896; WzxE_Proteobacteria.
DR Pfam; PF01943; Polysacc_synt; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..416
FT /note="Lipid III flippase"
FT /id="PRO_0000065990"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02024"
SQ SEQUENCE 416 AA; 44960 MW; 001690A4E86CADF5 CRC64;
MSLAKASLWT AASTLVKIGA GLLVGKLLAV SFGPAGLGLA ANFRQLITVL GVLAGAGIFN
GVTKYVAQYH DNPQQLRRVV GTSSAMVLGF STLMALVFVL AAAPISQGLF GNTDYQGLVR
LVALVQMGIA WGNLLLALMK GFRDAAGNAL SLIVGSLIGV LAYYVSYRLG GYEGALLGLA
LIPALVVIPA AIMLIKRGVI PLSYLKPSWD NGLAGQLSKF TLMALITSVT LPVAYIMMRK
LLAAQYSWDE VGIWQGVSSI SDAYLQFITA SFSVYLLPTL SRLTEKRDIT REVVKSLKFV
LPAVAAASFT VWLLRDFAIW LLLSNKFTAM RDLFAWQLVG DVLKVGAYVF GYLVIAKASL
RFYILAEVSQ FTLLMVFAHW LIPAHGALGA AQAYMATYIV YFSLCCGVFL LWRRRA
