WDFY4_MOUSE
ID WDFY4_MOUSE Reviewed; 3183 AA.
AC E9Q2M9;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2018, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=WD repeat- and FYVE domain-containing protein 4 {ECO:0000303|PubMed:30409884};
GN Name=Wdfy4 {ECO:0000303|PubMed:30409884};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0007744|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=30257884; DOI=10.4049/jimmunol.1800399;
RA Yuan Q., Li Y., Li J., Bian X., Long F., Duan R., Ma X., Gao F., Gao S.,
RA Wei S., Li X., Sun W., Liu Q.;
RT "WDFY4 is involved in symptoms of Systemic Lupus Erythematosus by
RT modulating B cell fate via noncanonical autophagy.";
RL J. Immunol. 201:2570-2578(2018).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH HSP90AB1, AND SUBCELLULAR
RP LOCATION.
RX PubMed=30409884; DOI=10.1126/science.aat5030;
RA Theisen D.J., Davidson J.T. IV, Briseno C.G., Gargaro M., Lauron E.J.,
RA Wang Q., Desai P., Durai V., Bagadia P., Brickner J.R., Beatty W.L.,
RA Virgin H.W., Gillanders W.E., Mosammaparast N., Diamond M.S., Sibley L.D.,
RA Yokoyama W., Schreiber R.D., Murphy T.L., Murphy K.M.;
RT "WDFY4 is required for cross-presentation in response to viral and tumor
RT antigens.";
RL Science 362:694-699(2018).
CC -!- FUNCTION: Plays a critical role in the regulation of cDC1-mediated
CC cross-presentation of viral and tumor antigens in dendritic cells
CC (PubMed:30409884). Mechanistically, acts near the plasma membrane and
CC interacts with endosomal membranes to promote endosomal-to-cytosol
CC antigen trafficking (PubMed:30409884). Also plays a role in B-cell
CC survival through regulation of autophagy (PubMed:30257884).
CC {ECO:0000269|PubMed:30257884, ECO:0000269|PubMed:30409884}.
CC -!- SUBUNIT: Interacts with HSP90AB1. {ECO:0000269|PubMed:30409884}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000269|PubMed:30409884}.
CC Endoplasmic reticulum {ECO:0000269|PubMed:30409884}.
CC -!- TISSUE SPECIFICITY: Highly expressed in immune tissues, especially B
CC lymphocytes. {ECO:0000269|PubMed:30257884}.
CC -!- DISRUPTION PHENOTYPE: Mice are unable to cross-present in vivo
CC (PubMed:30409884). In addition, affects the development of B-cells with
CC a block in the transition between the pro- and pre-B cell stages in
CC bone marrow (PubMed:30257884). {ECO:0000269|PubMed:30257884,
CC ECO:0000269|PubMed:30409884}.
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DR EMBL; AC133585; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154206; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC154239; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC165262; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_006519340.1; XM_006519277.3.
DR RefSeq; XP_006519341.1; XM_006519278.3.
DR RefSeq; XP_006519342.1; XM_006519279.3.
DR RefSeq; XP_011243420.1; XM_011245118.2.
DR SMR; E9Q2M9; -.
DR IntAct; E9Q2M9; 1.
DR MINT; E9Q2M9; -.
DR STRING; 10090.ENSMUSP00000117068; -.
DR iPTMnet; E9Q2M9; -.
DR PhosphoSitePlus; E9Q2M9; -.
DR EPD; E9Q2M9; -.
DR PeptideAtlas; E9Q2M9; -.
DR PRIDE; E9Q2M9; -.
DR ProteomicsDB; 312223; -.
DR Antibodypedia; 44930; 70 antibodies from 17 providers.
DR Ensembl; ENSMUST00000130509; ENSMUSP00000117068; ENSMUSG00000051506.
DR GeneID; 545030; -.
DR CTD; 57705; -.
DR MGI; MGI:3584510; Wdfy4.
DR VEuPathDB; HostDB:ENSMUSG00000051506; -.
DR eggNOG; KOG1786; Eukaryota.
DR eggNOG; KOG1788; Eukaryota.
DR GeneTree; ENSGT00940000155684; -.
DR HOGENOM; CLU_000175_5_0_1; -.
DR InParanoid; E9Q2M9; -.
DR OMA; CKSEGFV; -.
DR OrthoDB; 101142at2759; -.
DR PhylomeDB; E9Q2M9; -.
DR TreeFam; TF313658; -.
DR ChiTaRS; Wdfy4; mouse.
DR PRO; PR:E9Q2M9; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; E9Q2M9; protein.
DR Bgee; ENSMUSG00000051506; Expressed in mesenteric lymph node and 83 other tissues.
DR ExpressionAtlas; E9Q2M9; baseline and differential.
DR GO; GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0019882; P:antigen processing and presentation; IMP:MGI.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0036037; P:CD8-positive, alpha-beta T cell activation; IMP:MGI.
DR GO; GO:0098586; P:cellular response to virus; IMP:MGI.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF48371; SSF48371; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Autophagy; Endoplasmic reticulum; Endosome; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..3183
FT /note="WD repeat- and FYVE domain-containing protein 4"
FT /id="PRO_0000448268"
FT DOMAIN 2383..2508
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 2525..2819
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 2930..2969
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 2979..3018
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 3021..3060
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 3070..3108
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 3150..3183
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 790..811
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 938..977
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1828..1852
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2812..2836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 959..977
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1828..1845
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2812..2828
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3183 AA; 354331 MW; 080063B6471C052F CRC64;
MEAEDLSKTE DRPEDPGFQN EGQSPAVKPS FSLEGQSPGP SVLWDMLEQK FLDYQQLMPR
NPEERRKNLL SLLPLFLKAW EHSVGIICFR SLQRLAEDVS DQLAQEIQQA LAGKPAEQAR
AAAGQLLQWK SDADQDGNLL LKSVYVLTGT DSETLGRVVD SGLPALLLQC LYLFFAFPVE
KDDLLESDVQ GQRMFVQMLL NICSESQGLE GLLSGSELQS LLIATTCLRE HSCLFWKQPT
FCVLRAISKA QSPSVIQYLR TADCVRLSVQ NLSKLADTLP APEVSEAVSL ILNFVRDSYP
ISSALLLEFE NGEGYPLLLK VLLRYNGLTQ GVVEPHLEEL IELVMWLTTC GRSELKVFDS
VTYPQLEGFK FHQEASGVTV KNLQAFQVLQ NLFHRASDSV LCIQVLLAIK TMWAWNPRNF
FLLEWTLQPI SQFAEIIPLK PTPVQEHFFQ LLETLVFKLL YVPHEVLAKV QRLIKESSEL
SCTLVALRSI LRITASDRLF TDIFRDSGLL GLLLAQLRKQ AKIMRKSGNK ECSPDVQDPE
RELTYVMLST VVTLLQGSVR NAVVLKDHGM VPFIKIFLDD ECYRGPSLSI LEQLSVINAE
EYMSIIVGAL CSSTQGELQL KLDLLKSLLR ILETPKGHAA FRVSSGFNGL LSLLSDLEGS
LQVPEVTTCG AVSPSQTLEL VLHTLCVVSA ALHLDPVNEH FFRSNGLFEK LAEDLCLLGC
FGTPEEERTR WDSSSDMKAR PFMDLLSCAF SSSCQFPPRL QSCLQILSFL ESMASGTLHL
RGDLMEPARA GQEPSVDAQK AEAGGRQGKF KQWPDMEDRM DEGDVMIMHP GIICIMVRLL
PRLYLEDHPQ LSEEIQCSVA RHLLSLVKSE KNRQVMCEAG MLRTLMTFCP RTLSTGGSDL
HSILIRIFEK LGSQAIEPDV LRQFLGLGIR PPRSAAVKSL HLPPGHEDNP GCSGSCAATA
EKPTDSSPRP GGSQALRPSW ASQYSATALQ TTLSLISMTS PRNLQPQRAA LTPSFVEFDM
SSEGYGCLFT PTLSTVMGTS TEHSISGGTG SGAPRPFPPP GGLTFSCWFL ISRQANVMEG
HPLRFLTLVR HLARTEQPFV CFSISLCMDD LSLVVSTEEK EFQPLDAMEP EDEAEPSAGR
QLQVRCSQLL TCGQWYHLAV VVSKEMKRNC SVTTYLDGQA IGSAKMLYIQ ALPGSFFSMD
PSSFVDVYGY IGTPRVWKQK SSLTWRLGPA YLFEEDISAD TLALIIKLGP RYCGNFQAVH
LQGEDPDGEA TPLIAEERVS FGLYVPSSSI TSIMNIRNTY NEVDSRLIAK EMNISSRDNA
TPVFLLRNCA GHLSGPLRTL GAVAVGQLGV RVFHSSPAAS SLDYIGGPAI LLGLISLATD
DHTMYAAMKV LHSVLTSNAM CDYLMQHICG YQILAFLLRK KTSFLNHRIF QLILSVAGTA
ELGFRPSAVT NMCIFQHVLC NFELWTNTAD NLELTLFSHL LEILQSPREG PRNAEVAHQA
QLLPKLLFLF NEPSLALSKV STIIAILGCQ LKGHFNIQDL LRVGLFVIYT LKPSSVNERQ
ICLDGAQDPS RPAGSQTSGK AIWLRNQLLE MLFGVISSSQ LHLTSELKEQ VFLSLGPDWF
LLLLQGHLHP STTTLALKLL LYFLSSPPLR GRFRDGLSAG CWVENCMDGV DIVMDNLKSR
PAVPDQSPCL LPGFRVLNDF LAYHVLIPEV YLIVSSFFLQ TPLTELTNGP RENLDLMLQW
LLQKHHQQEV LQAGLCIEGA LLLLGMLKAI MNQPPAGSGD GAWEQTLPSS VLQFLRLVHR
SYPQDSAWRT PDFLQTVAII TFPLETQKET TSESSRNTSS PGASAEASHA AEGFQASFQP
HPALRQLREF MQVLLRELLL GGSNPKQWLP LEVILEASPD GATSQQKRDF QTEVLLSTMD
IFQVPSGDGM PTLRGSKEPL PNAEAGAVPS LASVSYFTQK LVEKIYSGVF SADPRHILLF
ITEHIIAVIE NPSSQKDTVM SALYSSLNKV ILHCLSKPQQ SLSECLGLLT ILDFLQEHWD
IIFATYNSNV SFLLCLMHCL LLLNARSYPE GFGLEPKPRI TPYHQVFLSP NEEVKDKKEE
GLPSLGDVQH SIQKSVRALW QQLVAQRRQT LEDAFKIDLS VKAGEIEVKI EEITPLWEET
MLRAWQHYLA SEKKSLASRS SVMHHSKVTS WSGSLSSAMR LMPGRQAKDP ECRAEDFVSC
IENYRRKGQE LYASIYKDYV QRRKSGSIKA ATAWARMREQ LFGELGLWGQ MTESTRCSRW
ELDGREGPAR MRKRIRHLLA WEPLNLGYKE SQEGKGDVSQ TNTGNQVFMT ADELTTEEAE
SRPDEVGVDC TQLTFFPALH ESLHSEDFLE LCRERQVILQ ELLDGEKVSQ KVPMVIVQGH
LVSEGILLFG QHHFYICENF TLSPTGDVYC THHCLSNISD PFIFNMCSKD RSSDHYSCQR
HAYSDLRELR QARFLLQDIA LEIFFQNGYS KLLVFYNSDR SKALKSFSTF QPSLKGKGTT
EDPFNLRKHP GFDRTMLQRW QKREISNFEY LMYLNTLAGR TYNDYMQYPV FPWVLADYTS
EMLNLTNPKT FRDLSKPMGA QTKERKLKFT QRFKDVEKIE GDMTVQCHYY THYSSAIIVA
SYLVRMPPFT QAFCSLQGGS FDVADRMFHS VKSTWESASK ENMSDVRELT PEFFYLPEFL
TNCNAVEFGC MQDGTTLGDV QLPPWADGDP RKFISLHRQA LESDFVSSNL HHWIDLIFGY
KQQGPAAVEA VNTFHPYFYG DRIDLGSITD PLIKSTILGF ISNFGQVPKQ IFTKPHPSRN
TTGKNPGPGK DASTPVGLPG HSQSFLHSLP ALRPSQVTVK DMYLFSLGSE SPKGAIGHIV
PTEKSILAVE KNKLLMPPLW NRTFSWGFDD FSCCLGSYGS DKILMTFENL AAWGPCLCAV
CPSPTMIVTS GASAVVCIWE LSLVKGRPRG LKLRQALYGH TQAVTCLTAS VTFSLLVSGS
QDRTCILWDL DHLSRVACLP VHREGISAIA ISDVSGTIVS CAGAHLSLWN VNGQPLASIT
TAWGPEGTIT CCCIVEGPAW DASHVIITGS KDGMVRIWKT EDVKMPVPRQ AVMEEPSTEP
LSPRGHKWAK NLALSRELDV SVALSGKPSK ASPAVTALAI TRNQSKLLVG DEKGRIFCWS
ADG
