ID   VPA_BPP2                Reviewed;         761 AA.
AC   Q06419;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   07-OCT-2020, entry version 81.
DE   RecName: Full=Replication gene A protein;
DE            EC=3.1.-.-;
DE   AltName: Full=GpA;
GN   Name=A;
OS   Escherichia phage P2 (Bacteriophage P2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Peduovirinae; Peduovirus.
OX   NCBI_TaxID=10679;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8510152; DOI=10.1006/jmbi.1993.1288;
RA   Liu Y., Saha S., Haggaard-Ljungquist E.;
RT   "Studies of bacteriophage P2 DNA replication. The DNA sequence of the cis-
RT   acting gene A and ori region and construction of a P2 mini-chromosome.";
RL   J. Mol. Biol. 231:361-374(1993).
RN   [2]
RP   SEQUENCE REVISION TO 34 AND 234.
RA   Christie G.E., Haggard-Ljungquist E., Calendar R.;
RT   "The complete genome of bacteriophage P2.";
RL   Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease which induces a single-strand cut at or near
CC       ori. May act by forming a covalent link to the 5'side of the nick.
CC   -!- DEVELOPMENTAL STAGE: Required continuously throughout the infection
CC       cycle.
CC   -!- SIMILARITY: Belongs to the phage GPA family. {ECO:0000305}.
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DR   EMBL; AF063097; AAD03306.1; -; Genomic_DNA.
DR   PIR; S33835; S33835.
DR   RefSeq; NP_046795.1; NC_001895.1.
DR   GeneID; 1261517; -.
DR   KEGG; vg:1261517; -.
DR   Proteomes; UP000009092; Genome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   InterPro; IPR008766; Replication_gene_A.
DR   Pfam; PF05840; Phage_GPA; 1.
PE   2: Evidence at transcript level;
KW   Covalent protein-DNA linkage; DNA replication; Early protein; Endonuclease;
KW   Hydrolase; Nuclease; Reference proteome; Viral DNA replication.
FT   CHAIN           1..761
FT                   /note="Replication gene A protein"
FT                   /id="PRO_0000165247"
FT   ACT_SITE        450
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        454
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   761 AA;  86523 MW;  02D2D322C833FDB4 CRC64;
     MAVKASGRFV PPSAFAAGTG KMFTGAYAWN APREAVGRER PLTRDEMRQM QGVLSTINRL
     PYFLRSLFTS RYDYIRRNKS PVHGFYFLTS TFQRRLWPRI ERVNQRHEMN TDASLLFLAE
     RDHYARLPGM NDKELKKFAA RISSQLFMMY EELSDAWVDA HGEKESLFTD EAQAHLYGHV
     AGAARAFNIS PLYWKKYRKG QMTTRQAYSA IARLFNDEWW THQLKGQRMR WHETLLIAVG
     EVNKDRSPYA SKHAIRDVRA RRQANLEFLK SCDLENRETG ERIDLISKVM GSISNPEIRR
     MELMNTIAGI ERYAAAEGDV GMFITLTAPS KYHPTRQVGK GESKTVQLNH GWNDEAFNPK
     DAQRYLCHIW SLMRTAFKDN DLQVYGLRVV EPHHDGTPHW HMMLFCNPRQ RNQIIEIMRR
     YALKEDGDER GAARNRFQAK HLNQGGAAGY IAKYISKNID GYALDGQLDN DTGRPLKDTA
     AAVTAWASTW RIPQFKTVGL PTMGAYRELR KLPRGVSIAD EFDERVEAAR AAADSGDFAL
     YISAQGGANV PRDCQTVRVA RSPSDEVNEY EEEVERVVGI YAPHLGARHI HITRTTDWRI
     VPKVPVVEPL TLKSGIAAPR SPVNNCGKLT GGDTSLPAPT PSEHAAAVLN LVDDGVIEWN
     EPEVVRALRG ALKYDMRTPN RQQRNGSPLK PHEIAPSARL TRSERLQITR IRVDLAQNGI
     RPQRWELEAL ARGATVNYDG KKFTYPVADE WPGFSTVMEW T