VP9D1_MOUSE
ID VP9D1_MOUSE Reviewed; 649 AA.
AC Q8C190; Q6PFD1;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=VPS9 domain-containing protein 1;
DE AltName: Full=5-day ovary-specific transcript 1 protein;
GN Name=Vps9d1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=ICR; TISSUE=Ovary;
RA Park C.E., Hwang K.C., Kim N.H., Lee K.A.;
RT "Identification of differentially expressed transcripts between 1 day ovary
RT and 5 day ovary.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Embryonic brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C190-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C190-2; Sequence=VSP_014703, VSP_014704;
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DR EMBL; AY751521; AAV30827.1; -; mRNA.
DR EMBL; AK028751; BAC26098.1; -; mRNA.
DR EMBL; BC057621; AAH57621.1; -; mRNA.
DR CCDS; CCDS52698.1; -. [Q8C190-1]
DR RefSeq; NP_001297590.1; NM_001310661.1.
DR RefSeq; NP_082476.1; NM_028200.2. [Q8C190-1]
DR AlphaFoldDB; Q8C190; -.
DR SMR; Q8C190; -.
DR BioGRID; 215310; 2.
DR STRING; 10090.ENSMUSP00000113575; -.
DR iPTMnet; Q8C190; -.
DR PhosphoSitePlus; Q8C190; -.
DR EPD; Q8C190; -.
DR MaxQB; Q8C190; -.
DR PaxDb; Q8C190; -.
DR PRIDE; Q8C190; -.
DR ProteomicsDB; 297929; -. [Q8C190-1]
DR ProteomicsDB; 297930; -. [Q8C190-2]
DR Antibodypedia; 1753; 35 antibodies from 8 providers.
DR Ensembl; ENSMUST00000117643; ENSMUSP00000113748; ENSMUSG00000001062. [Q8C190-2]
DR Ensembl; ENSMUST00000122363; ENSMUSP00000113575; ENSMUSG00000001062. [Q8C190-1]
DR GeneID; 72325; -.
DR KEGG; mmu:72325; -.
DR UCSC; uc009nuv.2; mouse. [Q8C190-1]
DR CTD; 9605; -.
DR MGI; MGI:1914143; Vps9d1.
DR VEuPathDB; HostDB:ENSMUSG00000001062; -.
DR eggNOG; KOG2319; Eukaryota.
DR GeneTree; ENSGT00390000015057; -.
DR HOGENOM; CLU_017849_0_0_1; -.
DR InParanoid; Q8C190; -.
DR OMA; RAICVCA; -.
DR OrthoDB; 1109212at2759; -.
DR PhylomeDB; Q8C190; -.
DR TreeFam; TF332037; -.
DR BioGRID-ORCS; 72325; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q8C190; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8C190; protein.
DR Bgee; ENSMUSG00000001062; Expressed in saccule of membranous labyrinth and 256 other tissues.
DR ExpressionAtlas; Q8C190; baseline and differential.
DR Genevisible; Q8C190; MM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0030139; C:endocytic vesicle; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0031267; F:small GTPase binding; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR Gene3D; 1.20.1050.80; -; 1.
DR InterPro; IPR036181; MIT_dom_sf.
DR InterPro; IPR003123; VPS9.
DR InterPro; IPR045046; Vps9-like.
DR InterPro; IPR037191; VPS9_dom_sf.
DR PANTHER; PTHR23101; PTHR23101; 1.
DR Pfam; PF02204; VPS9; 1.
DR SMART; SM00167; VPS9; 1.
DR SUPFAM; SSF109993; SSF109993; 1.
DR SUPFAM; SSF116846; SSF116846; 1.
DR PROSITE; PS51205; VPS9; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..649
FT /note="VPS9 domain-containing protein 1"
FT /id="PRO_0000079280"
FT DOMAIN 487..648
FT /note="VPS9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00550"
FT REGION 348..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..367
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 116
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 552..555
FT /note="VRTL -> GAGI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014703"
FT VAR_SEQ 556..649
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_014704"
SQ SEQUENCE 649 AA; 71284 MW; 34385AD36C050552 CRC64;
MAAAAGDGGA KPLQSAMKLA NKAIELDTGN RPREAYVEYL RSIHYISQVL LEDVENTTEA
GETVPPETSK MLKLAEQCLE RAQSTATKLG RICLKPAVPA APPTPLPTSR HRRVCSDEGG
KLSPFLPPEI FQKLQVAESQ NSKKELTPLE KASLQNQKLR ATYEARMARL DPSQAMQKTS
LTLSLQRQMM ENLVIAKARE ETLQRKMEER RLRLQEAANR RFCSQVALTP EEREQRALYA
AILEYEQDHD WPKHWRAKLK RSPGDLSLVT SLLSHLLSLP DHPISQLLKK LQCAVYSALY
PIVSRAATSA APAPGSCSLP PEANGLLAPG SRRLRSSQSL YCMLSPSEPS AAPRVLDGPP
PTPSVPTLHS GPLDREADGS PAGPSSPLVD ALSHLPDKDS SFEDLEQFLA MSEPQTPGAK
REPLLECLKS TVKDIHNAID RLLSLTLLAF ESLNTATSKD RCLACIEEPF FSPLWPLLLA
LYRSVHRTRE AAVSRSMELY RNAPPTALGI PTKLLPRVPE AQASTYPYCT AAQELGLLVL
ESCPQKKLEC IVRTLRVICI CAEDYCRAQE ARPEGESQPP AAAIGADDLL PILSFVVLRS
GLPQLVSECA ALEEFTHEGY LIGEEGYCLT SLQSALSYVE LLSRGRLDK
