VME1_CVH22
ID VME1_CVH22 Reviewed; 225 AA.
AC P15422; Q9DLM9;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 3.
DT 02-JUN-2021, entry version 97.
DE RecName: Full=Membrane protein {ECO:0000255|HAMAP-Rule:MF_04201};
DE Short=M protein {ECO:0000255|HAMAP-Rule:MF_04201};
DE AltName: Full=E1 glycoprotein {ECO:0000255|HAMAP-Rule:MF_04201};
DE AltName: Full=Matrix glycoprotein {ECO:0000255|HAMAP-Rule:MF_04201};
DE AltName: Full=Membrane glycoprotein {ECO:0000255|HAMAP-Rule:MF_04201};
GN Name=M {ECO:0000255|HAMAP-Rule:MF_04201}; ORFNames=6;
OS Human coronavirus 229E (HCoV-229E).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Alphacoronavirus; Duvinacovirus.
OX NCBI_TaxID=11137;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2818210; DOI=10.1007/bf01317928;
RA Raabe T., Siddell S.G.;
RT "Nucleotide sequence of the gene encoding the membrane protein of human
RT coronavirus 229 E.";
RL Arch. Virol. 107:323-328(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2305554; DOI=10.1016/0042-6822(90)90115-8;
RA Jouvenne P., Richardson C.D., Schreiber S.S., Lai M.M.C., Talbot P.J.;
RT "Sequence analysis of the membrane protein gene of human coronavirus
RT 229E.";
RL Virology 174:608-612(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11369870; DOI=10.1099/0022-1317-82-6-1273;
RA Thiel V., Herold J., Schelle B., Siddell S.G.;
RT "Infectious RNA transcribed in vitro from a cDNA copy of the human
RT coronavirus genome cloned in vaccinia virus.";
RL J. Gen. Virol. 82:1273-1281(2001).
CC -!- FUNCTION: Component of the viral envelope that plays a central role in
CC virus morphogenesis and assembly via its interactions with other viral
CC proteins. {ECO:0000255|HAMAP-Rule:MF_04201, ECO:0000255|PROSITE-
CC ProRule:PRU01275}.
CC -!- SUBUNIT: Homomultimer. Interacts with envelope E protein in the budding
CC compartment of the host cell, which is located between endoplasmic
CC reticulum and the Golgi complex. Forms a complex with HE and S
CC proteins. Interacts with nucleocapsid N protein. This interaction
CC probably participates in RNA packaging into the virus.
CC {ECO:0000255|HAMAP-Rule:MF_04201, ECO:0000255|PROSITE-
CC ProRule:PRU01275}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-
CC Rule:MF_04201}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_04201}. Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC Rule:MF_04201}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_04201}. Note=Largely embedded in the lipid bilayer.
CC {ECO:0000255|HAMAP-Rule:MF_04201}.
CC -!- SIMILARITY: Belongs to the alphacoronaviruses M protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04201}.
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DR EMBL; X15498; CAA33521.1; -; Genomic_RNA.
DR EMBL; M33560; AAA45461.1; -; Genomic_RNA.
DR EMBL; AF304460; AAG48596.1; -; Genomic_RNA.
DR PIR; A34127; MMIHHC.
DR RefSeq; NP_073555.1; NC_002645.1.
DR PRIDE; P15422; -.
DR DNASU; 918762; -.
DR GeneID; 918762; -.
DR KEGG; vg:918762; -.
DR Proteomes; UP000006716; Genome.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-UniRule.
DR CDD; cd21564; alphaCoV_M; 1.
DR HAMAP; MF_04201; ALPHA_CORONA_M; 1.
DR InterPro; IPR042551; ALPHA_CORONA_M.
DR InterPro; IPR002574; M_CoV.
DR Pfam; PF01635; CoV_M; 1.
DR PROSITE; PS51927; COV_M; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Host Golgi apparatus; Host membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix;
KW Viral envelope protein; Viral matrix protein; Virion.
FT CHAIN 1..225
FT /note="Membrane protein"
FT /id="PRO_0000106032"
FT TOPO_DOM 1..10
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT TOPO_DOM 32..40
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT TOPO_DOM 62..74
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT TOPO_DOM 96..225
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT REGION 199..215
FT /note="Interaction with N protein"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT CONFLICT 43
FT /note="F -> L (in Ref. 2; AAA45461)"
FT CONFLICT 81..82
FT /note="FF -> LL (in Ref. 2; AAA45461)"
FT CONFLICT 191
FT /note="S -> C (in Ref. 1; CAA33521)"
SQ SEQUENCE 225 AA; 25954 MW; CE60C6B5F23B0D27 CRC64;
MSNDNCTGDI VTHLKNWNFG WNVILTIFIV ILQFGHYKYS RLFYGLKMLV LWLLWPLVLA
LSIFDTWANW DSNWAFVAFS FFMAVSTLVM WVMYFANSFR LFRRARTFWA WNPEVNAITV
TTVLGQTYYQ PIQQAPTGIT VTLLSGVLYV DGHRLASGVQ VHNLPEYMTV AVPSTTIIYS
RVGRSVNSQN STGWVFYVRV KHGDFSAVSS PMSNMTENER LLHFF
