VITH2_ARATH
ID VITH2_ARATH Reviewed; 196 AA.
AC Q9SRD3;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Vacuolar iron transporter homolog 2 {ECO:0000305};
DE AltName: Full=Protein NODULIN-LIKE 2 {ECO:0000303|PubMed:21411332};
DE AltName: Full=Vacuolar iron transporter-like 2 {ECO:0000303|PubMed:25360591};
DE Short=AtVTL2 {ECO:0000303|PubMed:25360591};
GN Name=VTL2 {ECO:0000303|PubMed:25360591};
GN OrderedLocusNames=At1g76800 {ECO:0000312|Araport:AT1G76800};
GN ORFNames=F28O16.17 {ECO:0000312|EMBL:AAF04441.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=17028341; DOI=10.1534/genetics.106.061044;
RA Rampey R.A., Woodward A.W., Hobbs B.N., Tierney M.P., Lahner B., Salt D.E.,
RA Bartel B.;
RT "An Arabidopsis basic helix-loop-helix leucine zipper protein modulates
RT metal homeostasis and auxin conjugate responsiveness.";
RL Genetics 174:1841-1857(2006).
RN [6]
RP INDUCTION.
RX PubMed=21411332; DOI=10.1016/j.plaphy.2011.02.011;
RA Gollhofer J., Schlaewicke C., Jungnick N., Schmidt W., Buckhout T.J.;
RT "Members of a small family of nodulin-like genes are regulated under iron
RT deficiency in roots of Arabidopsis thaliana.";
RL Plant Physiol. Biochem. 49:557-564(2011).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=25360591; DOI=10.1371/journal.pone.0110468;
RA Gollhofer J., Timofeev R., Lan P., Schmidt W., Buckhout T.J.;
RT "Vacuolar-Iron-Transporter1-Like proteins mediate iron homeostasis in
RT Arabidopsis.";
RL PLoS ONE 9:E110468-E110468(2014).
CC -!- FUNCTION: Probable vacuolar iron transporter involved in the transfer
CC of iron ions from the cytosol to the vacuole for intracellular iron
CC storage (PubMed:25360591). Involved in regulation of cellular iron
CC homeostasis (PubMed:25360591). Vacuolar iron storage is required for
CC seed embryo and seedling development (PubMed:25360591).
CC {ECO:0000269|PubMed:25360591}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:25360591};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and inflorescences.
CC {ECO:0000269|PubMed:17028341}.
CC -!- INDUCTION: Down-regulated under iron deficiency (PubMed:21411332,
CC PubMed:25360591). Induced by iron supply (PubMed:25360591).
CC {ECO:0000269|PubMed:21411332, ECO:0000269|PubMed:25360591}.
CC -!- MISCELLANEOUS: Can mediate sequestration of iron ions into vacuoles
CC when expressed in the yeast ccc1 mutant. {ECO:0000269|PubMed:25360591}.
CC -!- SIMILARITY: Belongs to the CCC1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC010718; AAF04441.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35890.1; -; Genomic_DNA.
DR EMBL; BT014942; AAT47793.1; -; mRNA.
DR EMBL; BT015634; AAU15133.1; -; mRNA.
DR PIR; F96796; F96796.
DR RefSeq; NP_177806.1; NM_106330.4.
DR AlphaFoldDB; Q9SRD3; -.
DR SMR; Q9SRD3; -.
DR STRING; 3702.AT1G76800.1; -.
DR TCDB; 2.A.89.3.4; the vacuolar iron transporter (vit) family.
DR PaxDb; Q9SRD3; -.
DR PRIDE; Q9SRD3; -.
DR ProteomicsDB; 228609; -.
DR EnsemblPlants; AT1G76800.1; AT1G76800.1; AT1G76800.
DR GeneID; 844014; -.
DR Gramene; AT1G76800.1; AT1G76800.1; AT1G76800.
DR KEGG; ath:AT1G76800; -.
DR Araport; AT1G76800; -.
DR TAIR; locus:2030091; AT1G76800.
DR eggNOG; KOG4473; Eukaryota.
DR HOGENOM; CLU_038957_5_1_1; -.
DR InParanoid; Q9SRD3; -.
DR OMA; NCEIERE; -.
DR OrthoDB; 1353644at2759; -.
DR PhylomeDB; Q9SRD3; -.
DR PRO; PR:Q9SRD3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SRD3; baseline and differential.
DR Genevisible; Q9SRD3; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IDA:UniProtKB.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IDA:UniProtKB.
DR GO; GO:0030026; P:cellular manganese ion homeostasis; IBA:GO_Central.
DR GO; GO:0006880; P:intracellular sequestering of iron ion; IDA:UniProtKB.
DR GO; GO:0010039; P:response to iron ion; IEP:TAIR.
DR InterPro; IPR008217; Ccc1_fam.
DR PANTHER; PTHR31851; PTHR31851; 1.
DR Pfam; PF01988; VIT1; 2.
PE 2: Evidence at transcript level;
KW Ion transport; Iron; Iron transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..196
FT /note="Vacuolar iron transporter homolog 2"
FT /id="PRO_0000411008"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..59
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..112
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..139
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..196
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
SQ SEQUENCE 196 AA; 20585 MW; D5AA74A841358B72 CRC64;
MDQSGSNTNM DIEKESTTFD YSKRSQWLRA AVLGANDGLV STASLMMGVG AVKHDVKAMI
LSGFAGMVAG ACSMAIGEFV SVYSQYDIEV AQMERDSVEI EKEKLPSPMQ AAAASALAFS
AGAIVPLLAA AFVKEYKMRI ISVVVAVTVA LMVFGWLGAA LGKAPAVRSS ARVLFGGWLA
MAVTFGLTKL IGLYGL
