ID   VIRB4_BRUSU             Reviewed;         831 AA.
AC   Q9RPY1; G0KER4; Q8FXK3;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Type IV secretion system protein virB4;
GN   Name=virB4; OrderedLocusNames=BRA0066, BS1330_II0066;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA   O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA   Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT   "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT   Ptl type IV secretion systems is essential for intracellular survival of
RT   Brucella suis.";
RL   Mol. Microbiol. 33:1210-1220(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC   STRAIN=1330;
RX   PubMed=11830669; DOI=10.1073/pnas.032514299;
RA   Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA   Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA   O'Callaghan D.;
RT   "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
CC   -!- FUNCTION: The VirB system could be required for the establishment of
CC       the replication niche in the host.
CC   -!- INDUCTION: Specifically induced within macrophages by phagosome
CC       acidification. Induced at 37 degrees Celsius in minimal medium,
CC       suggesting that nutritional stress is a regulating signal.
CC   -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC       exponential phase.
CC   -!- SIMILARITY: Belongs to the TrbE/VirB4 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD56614.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF141604; AAD56614.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE014292; AAN33278.1; -; Genomic_DNA.
DR   EMBL; CP002998; AEM19558.1; -; Genomic_DNA.
DR   RefSeq; WP_004688809.1; NZ_KN046805.1.
DR   AlphaFoldDB; Q9RPY1; -.
DR   DIP; DIP-61162N; -.
DR   IntAct; Q9RPY1; 1.
DR   EnsemblBacteria; AEM19558; AEM19558; BS1330_II0066.
DR   GeneID; 45053163; -.
DR   KEGG; bms:BRA0066; -.
DR   KEGG; bsi:BS1330_II0066; -.
DR   PATRIC; fig|204722.21.peg.2304; -.
DR   HOGENOM; CLU_008341_3_0_5; -.
DR   OMA; HRDLNTL; -.
DR   PRO; PR:Q9RPY1; -.
DR   Proteomes; UP000007104; Chromosome II.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004346; CagE_TrbE_VirB.
DR   InterPro; IPR018145; CagE_TrbE_VirB_cntrl_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR043964; P-loop_TraG.
DR   Pfam; PF03135; CagE_TrbE_VirB; 1.
DR   Pfam; PF19044; P-loop_TraG; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00929; VirB4_CagE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Virulence.
FT   CHAIN           1..831
FT                   /note="Type IV secretion system protein virB4"
FT                   /id="PRO_0000290171"
FT   BINDING         457..464
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        80
FT                   /note="W -> R (in Ref. 1; AAD56614)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   831 AA;  94546 MW;  3C8D3695FA986EE4 CRC64;
     MGAQSKYAQQ LNNERSLAPF IPFRSQVGPT TVITRDGDFV RTWRIAGLAF ETQDKEELLI
     RKDQLNTLFR AIASNNVALW SHNVRRRTWD HLKSFFSNPF CDALDKKYYG SFSGYRMMSN
     ELYLTVIYRP VPAKISRLFN VAVHRSHAEI LQEQQLAIRK LDEIGNQIET SLRRYGGDDG
     RGIEVLSTYE DKHGALCSQQ LEFYNFLLSG EWQKVRVPSC PLDEYLGTGW VYAGTETIEI
     RTANATRYAR GIDFKDYASH TEPGILNGLM YSDYEYVITQ SFSFMTKRDG KEFLTRQKQR
     LQNTEDGSAS QIMEMDIAID QLGRGDFVMG EYHYSLLVFA EDMETVRHNT SHAMNILQDN
     GFLATVIATA TDAAFYAQLP CNWRYRPRVA GLTSLNFAGL SCFHNFRAGK RDGNPWGQAL
     TLLKTPSGQP AYLNFHYSKG DEDNFDKKLL GNTRIIGQSG AGKTVLMNFC LAQAQKYLHN
     APMGMCNVFF DKDQGAKGTI LAIGGKYLAI RNGEPTGFNP FQMEPTAGNI LFLEKLVQVL
     VSRDGQHVTT TDESRISHAI RTVMRMRPEL RRLSTVLQNV TEGSDRQDRE NSVAKRLAKW
     CFDDGTGKRG TFWWVLDCPQ DQIDFNTHSN YGFDGTDFLD NADVRTPISM YLLHRMELAI
     DGRRFIYWMD EAWKWVDDEA FSEFANNKQL TIRKQNGLGV FATQMPSSLL NSKVASALVQ
     QVATEIYLPN PKADYHEYTD GFKVTNEEFD IIRSMSEESR MFLVKQGHHS MICRLELNGF
     DDELAILSGS SDNNELLDQV IAEVGDDPSV WLPVFQERRK ARIASSKSTG R