ID   VELB_ASPFN              Reviewed;         361 AA.
AC   B8MYL0;
DT   16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Velvet complex subunit B {ECO:0000305};
GN   Name=velB {ECO:0000303|PubMed:23994319}; ORFNames=AFLA_081490;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND IDENTIFICATION IN THE VELVET COMPLEX.
RX   PubMed=23994319; DOI=10.1016/j.fgb.2013.08.009;
RA   Chang P.K., Scharfenstein L.L., Li P., Ehrlich K.C.;
RT   "Aspergillus flavus VelB acts distinctly from VeA in conidiation and may
RT   coordinate with FluG to modulate sclerotial production.";
RL   Fungal Genet. Biol. 58:71-79(2013).
CC   -!- FUNCTION: Component of the velvet transcription factor complex that
CC       controls sexual/asexual developmental ratio in response to light,
CC       promoting sexual development in the darkness while stimulating asexual
CC       sporulation under illumination (PubMed:23994319). The velvet complex
CC       acts as a global regulator for secondary metabolite gene expression (By
CC       similarity). Component of the velB-VosA heterodimeric complex that
CC       plays a dual role in activating genes associated with spore maturation
CC       and repressing certain development-associated genes (By similarity).
CC       The velB-VosA complex binds DNA through the DNA-binding domain of vosA
CC       that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3'
CC       consisting of two motifs in the promoters of key developmental
CC       regulatory genes (By similarity). Controls the expression of the
CC       aflatoxin gene cluster (PubMed:23994319). Likely coordinates with fluG
CC       to modulate sclerotial production (PubMed:23994319).
CC       {ECO:0000250|UniProtKB:C8VTS4, ECO:0000269|PubMed:23994319}.
CC   -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC       laeA, veA and velB; VeA acting as a bridging protein between laeA and
CC       velB (PubMed:23994319). Forms a heterodimeric complex with vosA; the
CC       formation of the velB-vosA complex is light-dependent (By similarity).
CC       {ECO:0000250|UniProtKB:C8VTS4, ECO:0000269|PubMed:23994319}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VTS4}. Cytoplasm
CC       {ECO:0000250|UniProtKB:C8VTS4}. Note=Nuclear localization is mediated
CC       by veA (By similarity). {ECO:0000250|UniProtKB:C8VTS4}.
CC   -!- DISRUPTION PHENOTYPE: Impairs conidiation, and abolishes sclerotial
CC       formation and aflatoxin production (PubMed:23994319).
CC       {ECO:0000269|PubMed:23994319}.
CC   -!- SIMILARITY: Belongs to the velvet family. VelB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; EQ963472; EED57452.1; -; Genomic_DNA.
DR   RefSeq; XP_002373064.1; XM_002373023.1.
DR   AlphaFoldDB; B8MYL0; -.
DR   SMR; B8MYL0; -.
DR   STRING; 5059.CADAFLAP00000929; -.
DR   EnsemblFungi; EED57452; EED57452; AFLA_081490.
DR   VEuPathDB; FungiDB:AFLA_081490; -.
DR   eggNOG; ENOG502S1B4; Eukaryota.
DR   HOGENOM; CLU_022491_0_0_1; -.
DR   OMA; KIGVWFI; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.3960; -; 2.
DR   InterPro; IPR021740; Velvet.
DR   InterPro; IPR037525; Velvet_dom.
DR   InterPro; IPR038491; Velvet_dom_sf.
DR   PANTHER; PTHR33572; PTHR33572; 2.
DR   Pfam; PF11754; Velvet; 1.
DR   PROSITE; PS51821; VELVET; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Sporulation; Transcription; Transcription regulation.
FT   CHAIN           1..361
FT                   /note="Velvet complex subunit B"
FT                   /id="PRO_0000435779"
FT   DOMAIN          54..340
FT                   /note="Velvet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT   REGION          1..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          139..161
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        7..29
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   361 AA;  39141 MW;  CC78590D4DB02325 CRC64;
     MYAIEERAHP PPPPPLSMDR IPPPSSSYPT PGSGGGMVSA GIPSSSHLAP LSTVHEGRIW
     SLQVVQQPIR ARMCGFGDKD RRPITPPPCI RLIVKDAQTE KEIDINEIDT SFYVLMVDLW
     NAEGTNEVNL VKHSATSPSI STAMSSSYPP PPQTLSPTYA QYPQNAYGQP VPYPQMNSYY
     PGNPQLQYQN PYGASPQTSY YQPYYPTGGH MPQANISPAQ PVSTGPGGMF TRNLIGSLSA
     SAFRLTDPDN KIGVWFILQD LSVRTEGVFR LKMSFVNVGT QSSDSPNGGV SVINHGSAPV
     LASVFSEPFQ VFSAKKFPGV IESTTLSKCF ALQGIKIPIR KDGVKGSRGR NNDDDDGDDY
     D