ID   USP_MANSE               Reviewed;         461 AA.
AC   P54779;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Protein ultraspiracle homolog;
DE   AltName: Full=Nuclear receptor subfamily 2 group B member 4;
GN   Name=USP; Synonyms=NR2B4;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Epidermis;
RX   PubMed=9013254; DOI=10.1046/j.1365-2583.1997.00153.x;
RA   Jindra M., Huang J.Y., Malone F., Asahina M., Riddiford L.M.;
RT   "Identification and mRNA developmental profiles of two ultraspiracle
RT   isoforms in the epidermis and wings of Manduca sexta.";
RL   Insect Mol. Biol. 6:41-53(1997).
CC   -!- FUNCTION: Receptor for ecdysone. May be an important modulator of
CC       insect metamorphosis (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of USP and ECR. Only the heterodimer is capable of
CC       high-affinity binding to ecdysone (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DOMAIN: Composed of three domains: a modulating N-terminal domain, a
CC       DNA-binding domain and a C-terminal ligand-binding domain.
CC   -!- SIMILARITY: Belongs to the nuclear hormone receptor family. NR2
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U44837; AAB64234.1; -; mRNA.
DR   AlphaFoldDB; P54779; -.
DR   SMR; P54779; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0003707; F:nuclear steroid receptor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   Gene3D; 1.10.565.10; -; 1.
DR   Gene3D; 3.30.50.10; -; 1.
DR   InterPro; IPR035500; NHR-like_dom_sf.
DR   InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR   InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR   InterPro; IPR000003; Retinoid-X_rcpt/HNF4.
DR   InterPro; IPR001628; Znf_hrmn_rcpt.
DR   InterPro; IPR013088; Znf_NHR/GATA.
DR   Pfam; PF00104; Hormone_recep; 1.
DR   Pfam; PF00105; zf-C4; 1.
DR   PRINTS; PR00545; RETINOIDXR.
DR   PRINTS; PR00398; STRDHORMONER.
DR   PRINTS; PR00047; STROIDFINGER.
DR   SMART; SM00430; HOLI; 1.
DR   SMART; SM00399; ZnF_C4; 1.
DR   SUPFAM; SSF48508; SSF48508; 1.
DR   PROSITE; PS51843; NR_LBD; 1.
DR   PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR   PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Metal-binding; Nucleus; Receptor; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..461
FT                   /note="Protein ultraspiracle homolog"
FT                   /id="PRO_0000053584"
FT   DOMAIN          203..452
FT                   /note="NR LBD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT   DNA_BIND        113..185
FT                   /note="Nuclear receptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT   ZN_FING         113..133
FT                   /note="NR C4-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT   ZN_FING         149..173
FT                   /note="NR C4-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT   REGION          1..112
FT                   /note="Modulating"
FT                   /evidence="ECO:0000250"
FT   REGION          26..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          185..192
FT                   /note="Hinge"
SQ   SEQUENCE   461 AA;  52066 MW;  2FD5318A5E963A55 CRC64;
     MSSVAKKDKR TMSVTALINR AWPLTPAPHQ QQSMPSSQPS NFLQPLATPS TTPSVELDIQ
     WLNIEPGFMS PMSPPEMKPD TAMLDGLRDD STPPPAFKNY PPNHPLSGSK HLCSICGDRA
     SGKHYGVYSC EGCKGFFKRT VRKDLTYACR EDRNCIIDKR QRNRCQYCRY QKCLACGMKR
     EAVQEERQRA ARGTEDAHPS SSVQELSIER LLEIESLVAD PPEEFQFLRV GPESGVPAKY
     RAPVSSLCQI GNKQIAALVV WARDIPHFGQ LELEDQILLI KNSWNELLLF AIAWRSMEYL
     TDERENVDSR STAPPQLMCL MPGMTLHRNS ALQAGVGQIF DRVLSELSLK MRTLRMDQAE
     YVALKAIILL NPDVKGLKNK PEVVVLREKM FSCLDEYVRR SRCAEEGRFA ALLLRLPALR
     SISLKCFEHL YFFHLVADTS IASYIHDALR NHAPSIDTSI L