ID   US9_BHV1S               Reviewed;         158 AA.
AC   Q08098;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   23-FEB-2022, entry version 63.
DE   RecName: Full=Envelope protein US9;
GN   ORFNames=US9;
OS   Bovine herpesvirus 1.2 (strain ST) (BoHV-1) (Infectious bovine
OS   rhinotracheitis virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=45407;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8122370; DOI=10.1006/viro.1994.1139;
RA   Leung-Tack P., Audonnet J.F., Riviere M.;
RT   "The complete DNA sequence and the genetic organization of the short unique
RT   region (US) of the bovine herpesvirus type 1 (ST strain).";
RL   Virology 199:409-421(1994).
RN   [2]
RP   TOPOLOGY, AND SUBCELLULAR LOCATION.
RC   STRAIN=Jura;
RX   PubMed=19420087; DOI=10.1128/jvi.00598-09;
RA   Lyman M.G., Kemp C.D., Taylor M.P., Enquist L.W.;
RT   "Comparison of the pseudorabies virus Us9 protein with homologs from other
RT   veterinary and human alphaherpesviruses.";
RL   J. Virol. 83:6978-6986(2009).
CC   -!- FUNCTION: Essential for the anterograde spread of the infection
CC       throughout the host nervous system. Together with the gE/gI
CC       heterodimer, US9 is involved in the sorting and transport of viral
CC       structural components toward axon tips (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Host Golgi apparatus membrane
CC       {ECO:0000269|PubMed:19420087}; Single-pass type II membrane protein
CC       {ECO:0000269|PubMed:19420087}. Host smooth endoplasmic reticulum
CC       membrane {ECO:0000250}; Single-pass type II membrane protein
CC       {ECO:0000250}. Host cell membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}. Note=During virion morphogenesis, this
CC       protein probably accumulates in the endosomes and trans-Golgi where
CC       secondary envelopment occurs. It is probably transported to the cell
CC       surface from where it is endocytosed and directed to the trans-Golgi
CC       network (TGN), maybe through an interaction with PACS-1 sorting
CC       protein.
CC   -!- PTM: Phosphorylated on serines within the acidic cluster, possibly by
CC       host CK2. Phosphorylation determines whether endocytosed viral US9
CC       traffics to the trans-Golgi network or recycles to the cell membrane.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the alphaherpesvirinae envelope protein US9
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z23068; CAA80607.1; -; Genomic_DNA.
DR   PIR; S35787; S35787.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044171; C:host cell smooth endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0075733; P:intracellular transport of virus; IEA:InterPro.
DR   InterPro; IPR009278; Herpes_US9.
DR   Pfam; PF06072; Herpes_US9; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host endoplasmic reticulum; Host Golgi apparatus;
KW   Host membrane; Membrane; Phosphoprotein; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..158
FT                   /note="Envelope protein US9"
FT                   /id="PRO_0000116138"
FT   TOPO_DOM        1..134
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        135..155
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        156..158
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          22..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          59..84
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          93..108
FT                   /note="Acidic"
FT   MOTIF           86..89
FT                   /note="Internalization motif"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         99
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         101
FT                   /note="Phosphoserine; by host CK2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   158 AA;  16266 MW;  01870BECC322B2F4 CRC64;
     MERSHKASCG CFEGMESPRS VVNENYRGAD EADAAPPSPP PEGSIVSIPI LELTIEDAPA
     SAEATGTAAA APAGRTPDAN AAPGGYVPVP AADADCYYSE SDSETAGEFL IRMGRQQRRR
     HRRRRCMIAA ALTCIGLGAC AAAAAAGAVL ALEVVPRP