UNC42_CAEEL
ID UNC42_CAEEL Reviewed; 279 AA.
AC L8E946; G5EBS8; N1NSG8; N1NTL5; N1NVB9;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Homeobox protein unc-42 {ECO:0000305};
DE AltName: Full=Uncoordinated protein 42 {ECO:0000312|WormBase:F58E6.10b};
GN Name=unc-42 {ECO:0000312|WormBase:F58E6.10b};
GN ORFNames=F58E6.10 {ECO:0000312|WormBase:F58E6.10b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF VAL-146; 147-TRP--ILE-279 AND
RP 107-PHE--ILE-279.
RX PubMed=9216999; DOI=10.1242/dev.124.13.2571;
RA Wightman B., Baran R., Garriga G.;
RT "Genes that guide growth cones along the C. elegans ventral nerve cord.";
RL Development 124:2571-2580(1997).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF VAL-146; 147-TRP--ILE-279
RP AND 107-PHE--ILE-279.
RX PubMed=10207148; DOI=10.1242/dev.126.10.2241;
RA Baran R., Aronoff R., Garriga G.;
RT "The C. elegans homeodomain gene unc-42 regulates chemosensory and
RT glutamate receptor expression.";
RL Development 126:2241-2251(1999).
RN [4] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 107-PHE--ILE-279 AND VAL-146.
RX PubMed=11222641; DOI=10.1523/jneurosci.21-05-01510.2001;
RA Brockie P.J., Madsen D.M., Zheng Y., Mellem J., Maricq A.V.;
RT "Differential expression of glutamate receptor subunits in the nervous
RT system of Caenorhabditis elegans and their regulation by the homeodomain
RT protein UNC-42.";
RL J. Neurosci. 21:1510-1522(2001).
RN [5] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 107-PHE--ILE-279.
RX PubMed=16183052; DOI=10.1016/j.ydbio.2005.08.032;
RA Wightman B., Ebert B., Carmean N., Weber K., Clever S.;
RT "The C. elegans nuclear receptor gene fax-1 and homeobox gene unc-42
RT coordinate interneuron identity by regulating the expression of glutamate
RT receptor subunits and other neuron-specific genes.";
RL Dev. Biol. 287:74-85(2005).
CC -!- FUNCTION: Probable transcription factor that is required for initial
CC outgrowth and pathfinding of axon growth cones along the ventral nerve
CC cord (VNC) (PubMed:9216999, PubMed:11222641). Involved in specifying
CC neuron identity, perhaps in concert with nuclear hormone receptor
CC family member fax-1 (PubMed:10207148, PubMed:16183052). Plays a role in
CC locomotion and mechanosensory response, perhaps via regulation of
CC chemosensory receptor expression, and is required for expression of
CC glutamate receptors, including glr-1, glr-4 and glr-5 (PubMed:10207148,
CC PubMed:11222641). {ECO:0000269|PubMed:10207148,
CC ECO:0000269|PubMed:11222641, ECO:0000269|PubMed:16183052,
CC ECO:0000269|PubMed:9216999}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|RuleBase:RU000682}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=b {ECO:0000312|WormBase:F58E6.10b};
CC IsoId=L8E946-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F58E6.10a};
CC IsoId=L8E946-2; Sequence=VSP_061080;
CC Name=e {ECO:0000312|WormBase:F58E6.10e};
CC IsoId=L8E946-3; Sequence=VSP_061079;
CC Name=d {ECO:0000312|WormBase:F58E6.10d};
CC IsoId=L8E946-4; Sequence=VSP_061079, VSP_061080;
CC Name=c {ECO:0000312|WormBase:F58E6.10c};
CC IsoId=L8E946-5; Sequence=VSP_061078;
CC -!- DEVELOPMENTAL STAGE: Expressed in neurons from 260 minutes of
CC embryogenesis, comma stage and into adulthood (at protein level)
CC (PubMed:10207148). Expressed in late stage (3 and a half-fold) embryos
CC in at least 20 pairs of neurons of the head, including the AVA, AVD and
CC AVE interneurons, ASH sensory neurons, and RMD and SMB motor neurons
CC (PubMed:10207148). Expressed at high levels in the AIN, AVH, AVJ, AVK,
CC RIV, SAA and SIB interneurons (PubMed:10207148). At larval L2 stage,
CC expressed in the tail in the PVT neuron (PubMed:10207148).
CC {ECO:0000269|PubMed:10207148}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; BX284605; CAA94780.3; -; Genomic_DNA.
DR EMBL; BX284605; CCQ25677.1; -; Genomic_DNA.
DR EMBL; BX284605; CCW45994.1; -; Genomic_DNA.
DR EMBL; BX284605; CCW45995.1; -; Genomic_DNA.
DR EMBL; BX284605; CCW45996.1; -; Genomic_DNA.
DR PIR; T22296; T22296.
DR RefSeq; NP_001263845.1; NM_001276916.1. [L8E946-1]
DR RefSeq; NP_001294700.1; NM_001307771.1. [L8E946-4]
DR RefSeq; NP_001294701.1; NM_001307772.1. [L8E946-5]
DR RefSeq; NP_001294702.1; NM_001307773.1. [L8E946-3]
DR RefSeq; NP_505519.3; NM_073118.5. [L8E946-2]
DR AlphaFoldDB; L8E946; -.
DR SMR; L8E946; -.
DR IntAct; L8E946; 18.
DR STRING; 6239.F58E6.10b; -.
DR EnsemblMetazoa; F58E6.10a.1; F58E6.10a.1; WBGene00006778. [L8E946-2]
DR EnsemblMetazoa; F58E6.10b.1; F58E6.10b.1; WBGene00006778. [L8E946-1]
DR EnsemblMetazoa; F58E6.10c.1; F58E6.10c.1; WBGene00006778. [L8E946-5]
DR EnsemblMetazoa; F58E6.10d.1; F58E6.10d.1; WBGene00006778. [L8E946-4]
DR EnsemblMetazoa; F58E6.10e.1; F58E6.10e.1; WBGene00006778. [L8E946-3]
DR GeneID; 192081; -.
DR KEGG; cel:CELE_F58E6.10; -.
DR CTD; 192081; -.
DR WormBase; F58E6.10a; CE39164; WBGene00006778; unc-42.
DR WormBase; F58E6.10b; CE48141; WBGene00006778; unc-42.
DR WormBase; F58E6.10c; CE48371; WBGene00006778; unc-42.
DR WormBase; F58E6.10d; CE48350; WBGene00006778; unc-42.
DR WormBase; F58E6.10e; CE48409; WBGene00006778; unc-42.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000165391; -.
DR HOGENOM; CLU_1125699_0_0_1; -.
DR InParanoid; L8E946; -.
DR OMA; FWYQSYQ; -.
DR OrthoDB; 1351285at2759; -.
DR SignaLink; L8E946; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00006778; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; L8E946; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061564; P:axon development; IMP:UniProtKB.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IMP:UniProtKB.
DR GO; GO:0048665; P:neuron fate specification; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..279
FT /note="Homeobox protein unc-42"
FT /id="PRO_0000452987"
FT DNA_BIND 100..159
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT VAR_SEQ 1..180
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061078"
FT VAR_SEQ 1..55
FT /note="MSDNLLNGANGASTVSQFQEKVKDLGVSLHDFTAYYPSSLDTVSASLRPISD
FT PSS -> MYYY (in isoform e and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_061079"
FT VAR_SEQ 85..99
FT /note="Missing (in isoform a and isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_061080"
FT MUTAGEN 107..279
FT /note="Missing: In e419; axon outgrowth and pathfinding
FT defects; mechanosensory defects; abolishes expression of
FT oligopeptide transporter pept-3 in AVE neurons, and both
FT FMRFamide related neuropeptide precursor gene flp-1 and
FT neuron specific calcium sensor ncs-1 in AVK neurons.
FT Abolishes expression of seven-transmembrane receptor srb in
FT ASH neurons."
FT /evidence="ECO:0000269|PubMed:10207148,
FT ECO:0000269|PubMed:11222641, ECO:0000269|PubMed:16183052,
FT ECO:0000269|PubMed:9216999"
FT MUTAGEN 146
FT /note="V->E: In e270; axon outgrowth, pioneering and
FT pathfinding defects; mechanosensory defects."
FT /evidence="ECO:0000269|PubMed:10207148,
FT ECO:0000269|PubMed:11222641, ECO:0000269|PubMed:9216999"
FT MUTAGEN 147..279
FT /note="Missing: In gm23; axon pioneering and pathfinding
FT defects."
FT /evidence="ECO:0000269|PubMed:10207148,
FT ECO:0000269|PubMed:9216999"
SQ SEQUENCE 279 AA; 31133 MW; 888F5894821EC41A CRC64;
MSDNLLNGAN GASTVSQFQE KVKDLGVSLH DFTAYYPSSL DTVSASLRPI SDPSSDGAFK
KIKTEGLGGS VFGSSIAGVT NTPARLCSLE RPESERLNSR RRHRTTFTQE QLQELDAAFQ
KSHYPDIYVR EELARITKLN EARIQVWFQN RRAKHRKHEK QLNKAINPPH SFLSNPANTL
MRQGMYPAAL NRDGFWYQSY QRPMPYPTAS PSYSNSFTNP IANFGHSITS FQADDEFYQK
SLALRMTTTP SAATAATSLA NINYQQTQPS EASTNPPSI
