UGPA_ECOLI
ID UGPA_ECOLI Reviewed; 295 AA.
AC P10905; Q2M7B5;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=sn-glycerol-3-phosphate transport system permease protein UgpA;
GN Name=ugpA; OrderedLocusNames=b3452, JW3417;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3062310; DOI=10.1111/j.1365-2958.1988.tb00088.x;
RA Overduin P., Boos W., Tommassen J.;
RT "Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to
RT the maltose system.";
RL Mol. Microbiol. 2:767-775(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Part of the binding-protein-dependent transport system for
CC sn-glycerol-3-phosphate; probably responsible for the translocation of
CC the substrate across the membrane.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (UgpC),
CC two transmembrane proteins (UgpA and UgpE) and a solute-binding protein
CC (UgpB). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. UgpAE subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X13141; CAA31532.1; -; Genomic_DNA.
DR EMBL; U00039; AAB18427.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76477.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77841.1; -; Genomic_DNA.
DR PIR; S03781; MMECUA.
DR RefSeq; NP_417909.1; NC_000913.3.
DR RefSeq; WP_000099289.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P10905; -.
DR SMR; P10905; -.
DR BioGRID; 4263421; 175.
DR ComplexPortal; CPX-2150; sn-glycerol-3-phosphate ABC transporter complex.
DR DIP; DIP-11077N; -.
DR IntAct; P10905; 1.
DR STRING; 511145.b3452; -.
DR TCDB; 3.A.1.1.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; P10905; -.
DR EnsemblBacteria; AAC76477; AAC76477; b3452.
DR EnsemblBacteria; BAE77841; BAE77841; BAE77841.
DR GeneID; 67417079; -.
DR GeneID; 947957; -.
DR KEGG; ecj:JW3417; -.
DR KEGG; eco:b3452; -.
DR PATRIC; fig|1411691.4.peg.3275; -.
DR EchoBASE; EB1039; -.
DR eggNOG; COG1175; Bacteria.
DR HOGENOM; CLU_016047_0_2_6; -.
DR InParanoid; P10905; -.
DR OMA; LWYSVQS; -.
DR PhylomeDB; P10905; -.
DR BioCyc; EcoCyc:UGPA-MON; -.
DR BioCyc; MetaCyc:UGPA-MON; -.
DR BRENDA; 7.6.2.10; 2026.
DR PRO; PR:P10905; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IDA:EcoCyc.
DR GO; GO:1902517; C:glycerol-3-phosphate-transporting ATPase complex; IPI:ComplexPortal.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015169; F:glycerol-3-phosphate transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0001406; F:glycerophosphodiester transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015794; P:glycerol-3-phosphate transmembrane transport; IDA:ComplexPortal.
DR GO; GO:0001407; P:glycerophosphodiester transmembrane transport; IDA:EcoCyc.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..295
FT /note="sn-glycerol-3-phosphate transport system permease
FT protein UgpA"
FT /id="PRO_0000060232"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 33..80
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 102..109
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 131..156
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 178..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 229..262
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 284..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 76..284
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 295 AA; 33264 MW; CC8765DE0FB647A9 CRC64;
MSSSRPVFRS RWLPYLLVAP QLIITVIFFI WPAGEALWYS LQSVDPFGFS SQFVGLDNFV
TLFHDSYYLD SFWTTIKFST FVTVSGLLVS LFFAALVEYI VRGSRFYQTL MLLPYAVAPA
VAAVLWIFLF NPGRGLITHF LAEFGYDWNH AQNSGQAMFL VVFASVWKQI SYNFLFFYAA
LQSIPRSLIE AAAIDGAGPI RRFFKIALPL IAPVSFFLLV VNLVYAFFDT FPVIDAATSG
GPVQATTTLI YKIYREGFTG LDLASSAAQS VVLMFLVIVL TVVQFRYVES KVRYQ
